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Licensed Unlicensed Requires Authentication Published by De Gruyter April 13, 2018

Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters

Fabiola del Rocío Villalobos-Gómez, Mario García-Lorenzana, Galileo Escobedo, Patricia Talamás-Rohana, Rogelio Salinas-Gutiérrez, Verónica-Ivonne Hernández-Ramírez, Esperanza Sánchez-Alemán, María del Rosario Campos-Esparza, Martín Humberto Muñoz-Ortega and Javier Ventura-Juárez
From the journal Acta Parasitologica


The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1β, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1β, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.

  1. Conflict of interest

    Conflict of interest statement: The authors have not interest conflict


This work was financed by a grant (number PIBB11-3) from the Universidad Autónoma de Aguascalientes and a basic science grant (number 134487) from CONACYT, as well as by a CONACYT fellowship (number 231571) for Villalobos-Gómez FR as part of the PhD program (Doctorado en Ciencias Biológicas y de la Salud) of the Universidad Autónoma Metropolitana


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Received: 2017-7-4
Revised: 2017-12-13
Accepted: 2018-1-17
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS

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