Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter April 13, 2018

Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters

Fabiola del Rocío Villalobos-Gómez, Mario García-Lorenzana, Galileo Escobedo, Patricia Talamás-Rohana, Rogelio Salinas-Gutiérrez, Verónica-Ivonne Hernández-Ramírez, Esperanza Sánchez-Alemán, María del Rosario Campos-Esparza, Martín Humberto Muñoz-Ortega and Javier Ventura-Juárez
From the journal Acta Parasitologica

Abstract

The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1β, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1β, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.



  1. Conflict of interest

    Conflict of interest statement: The authors have not interest conflict

Acknowledgment

This work was financed by a grant (number PIBB11-3) from the Universidad Autónoma de Aguascalientes and a basic science grant (number 134487) from CONACYT, as well as by a CONACYT fellowship (number 231571) for Villalobos-Gómez FR as part of the PhD program (Doctorado en Ciencias Biológicas y de la Salud) of the Universidad Autónoma Metropolitana

References

Amulic B., Cazalet C., Hayes G.L., Metzler K.D., Zychlinsky A. 2012. Neutrophil function: from mechanisms to disease. Annual Review of Immunology, 30, 459–489. 10.1146/annurev-immunol-020711-074942Search in Google Scholar PubMed

Báez-Pagán C.A., Delgado-Vélez A.M., Lasalde-Dominicci J.A. 2015. Activation of the Macrophage α7 Nicotinic Acetylcholine Receptor and Control of Inflammation. Journal of Neuroimmune Pharmacology: The Official Journal of the Society on NeuroImmune Pharmacology, 10, 468–476. 10.1007/s11481-015-9601-5Search in Google Scholar PubMed PubMed Central

Bekker-Mendez V.C., Pérez-Castillo V.L., Rico-Rosillo M.G., Pérez-Rodríguez M., Arellano-Blanco J., Kretschmer-Schmid R.R., and Talamás-Rohana P. 2006. Downregulation of selected cytokines in amebiasis. Archives of Medical Research,37, 556–810.1016/j.arcmed.2005.09.018Search in Google Scholar PubMed

Blazquez S., Zimmer C., Guigon G., Olivo-Marin J.C., Guillén N., Labruyère E. 2006. Human Tumor Necrosis Factor is a Chemoattractant for the Parasite Entamoeba histolytica. Infection and Immunity, 74, 1407. 10.1128/IAI.74.2.1407-1411.2006Search in Google Scholar PubMed PubMed Central

Chadee K., Meerovitch E., Moreau F. 1985. In vitro and in vivo interaction between trophozoites of Entamoeba histolytica and gerbil lymphoid cells. Infection and Immunity, 49, 828–83210.1128/iai.49.3.828-832.1985Search in Google Scholar PubMed PubMed Central

Diamond L. S. 1961. Axenic cultivation of Entamoeba histolytica. Science, 134, 336–33710.1126/science.134.3475.336Search in Google Scholar PubMed

Elenkov I. J. 2008. Neurohormonal-cytokine interactions: implications for inflammation, common human diseases and wellbeing. Neurochemistry International, 52, 40–51. 10.1016/j.neuint.2007.06.037Search in Google Scholar PubMed

Engler K. L., Rudd M.L., Ryan J.J., Stewart J.K., Fischer-Stenger K. 2005. Autocrine actions of macrophage-derived catecholamines on interleukin-1 beta. Journal of Neuroimmunology, 160, 87–91. 10.1016/j.jneuroim.2004.11.005Search in Google Scholar PubMed

Espinosa-Cantellano M., Martínez-Palomo A. 2000. Pathogenesis of Intestinal Amebiasis: From Molecules to Disease. Clinical Microbiology Reviews, 13, 318–33110.1128/CMR.13.2.318-331.2000Search in Google Scholar PubMed

Gahring L.C., Osborne A.V., Reed M., Rogers S.W. 2010. Neuronal nicotinic alpha7 receptors modulate early neutrophil infiltration to sites of skin inflammation. Journal of Neuroinflammation, 7, 38. 10.1186/1742-2094-7-38Search in Google Scholar PubMed PubMed Central

Geissmann F., Manz M.G., Jung S., Sieweke M.H., Merad M., Ley K. 2010. Development of monocytes, macrophages, and dendritic cells. Science, 327, 656–661. 10.1126/science.1178331Search in Google Scholar PubMed PubMed Central

Gersuk G.M., Razai L.W., Marr K.A. 2008. Methods of in vitro macrophage maturation confer variable inflammatory responses in association with altered expression of cell surface dectin-1. Journal of Immunological Methods, 329, 157–166. 10.1016/j.jim.2007.10.003Search in Google Scholar PubMed

Ghause M.S., Singh V., Kumar A., Wahal R., Bhatia V.K., Agarwal J. 2002. A study of cardiovascular response during laryngoscopy and intubation and their attenuation by ultra short acting b-blocker esmolol. Journal of Anesthesia, 46, 104–6Search in Google Scholar

Herman R.A., Scherer P.N., Shan G. 2008. Evaluation of logistic and polynomial models for fitting sandwich-ELISA calibration curves. Journal of Immunological Methods, 339, 245–258. 10.1016/j.jim.2008.09.001Search in Google Scholar PubMed

Jay G.T., Chow M.S. 1995. Interaction of epinephrine and betablockers. Journal of the American Medical Association, 274, 1830–183210.1001/jama.1995.03530230016008Search in Google Scholar

Johnston L., Harding S.A., La Flamme A.C. 2015. Comparing methods for ex vivo characterization of human monocyte phenotypes and in vitro responses. Immunobiology, 220, 1305–1310. 10.1016/j.imbio.2015.07.014Search in Google Scholar PubMed

Kawai T., Akira S. 2009. The roles of TLRs, RLRs and NLRs in pathogen recognition. International Immunology, 21, 317– 337. 10.1093/intimm/dxp017Search in Google Scholar PubMed PubMed Central

Lejeune M., Rybicka J.M., Chadee K. 2009. Recent discoveries in the pathogenesis and immune response toward Entamoeba histolytica. Future Microbiology, 4, 105–118. 10.2217/17460913.4.1.105Search in Google Scholar PubMed

Maldonado-Bernal C., Kirschning C.J., Rosenstein Y., Rocha L.M., Rios-Sarabia N., Espinosa-Cantellano M., et al. 2005. The innate immune response to Entamoeba histolytica lipopeptidophosphoglycan is mediated by toll-like receptors 2 and 4. Parasite Immunology, 27, 127–137. 10.1111/j.1365-3024.2005.00754.xSearch in Google Scholar PubMed

Martinez F. O., Helming L., S. Gordon S. 2009. Alternative activation of macrophages: an immunologic functional perspective. Annual Review of Immunology, 27, 451–483. 10.1146/annurev.immunol.021908.132532Search in Google Scholar PubMed

Meyer-Wentrup F., Burdach S. 2003. Efficacy of dendritic cell generation for clinical use: recovery and purity of monocytes and mature dendritic cells after immunomagnetic sorting or adherence selection of CD14+ starting populations. Journal of Hematotherapy & Stem Cell Research, 12, 289–299. 10.1089/152581603322023025Search in Google Scholar PubMed

Moraes L. C. A., França E.L., Pessoa R.S., Fagundes D.L.G., Hernandes M.G., Ribeiro V.P., et al. 2015. The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica. Parasites & Vectors, 8, 1–8. 10.1186/s13071-015-1028-6Search in Google Scholar PubMed PubMed Central

Muñoz-Ortega M., Quintanar-Stephano A., García Lorenzana M., Campos-Esparza M.R., Silva-Briano M., Adabache-Ortíz A., et al. 2011. Modulation of amoebic hepatic abscess by the parasympathetic system. Parasite Immunology, 33, 65–7210.1111/j.1365-3024.2010.01245.xSearch in Google Scholar PubMed

Pacheco-Yépez J., Rivera-Aguilar V., Barbosa-Cabrera E., Rojas Hernández S., Jarillo-Luna R.A., Campos-Rodríguez R. 2011. Myeloperoxidase binds to and kills Entamoeba histolytica trophozoites. Parasite Immunology, 33, 255–264. 10.1111/j.1365-3024.2010.01275.xSearch in Google Scholar PubMed

Pilling D., Fan T., Huang D., Kaul B., Gomer R.H. 2009. Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts. PloS One, 4, e7475. 10.1371/journal.pone.0007475Search in Google Scholar PubMed PubMed Central

Punshon G., Vara D.S., Sales K.M., Seifalian A.M. 2011. A novel method for the extraction and culture of progenitor stem cells from human peripheral blood for use in regenerative medicine. Biotechnology and Applied Biochemistry, 58, 328–334. 10.1002/bab.47Search in Google Scholar PubMed

Ravdin J. I., Guerrant R.L. 1981. Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. Study with mammalian tissue culture cells and human erythrocytes. The Journal of Clinical Investigation, 68, 1305–131310.1172/JCI110377Search in Google Scholar PubMed PubMed Central

Rosales-Encina J.L., Meza I., López-De-León A., Talamás-Rohana P., Rojkind M. 1987. Isolation of a 220-kilodalton protein with lectin properties from a virulent strain of Entamoeba histolytica. The Journal of Infectious Diseases, 5, 790–79710.1093/infdis/156.5.790Search in Google Scholar PubMed

Rosas-Ballina M., Tracey K.J. 2009. The neurology of the immune system: neural reflexes regulate immunity. Neuron, 64, 28–32. 10.1016/j.neuron.2009.09.039Search in Google Scholar PubMed PubMed Central

Salata R. A., Murray H.W., Rubin B.Y., Ravdin J.I. 1987. The role of gamma interferon in the generation of human macrophages cytotoxic for Entamoeba histolytica trophozoites. The American Journal of Tropical Medicine and Hygiene, 37, 72–7810.4269/ajtmh.1987.37.72Search in Google Scholar PubMed

Spangelo, B. L., A. M. Judd, Call G.B., Zumwalt J., Gorospe W.C. 1995. Role of the cytokines in the hypothalamic-pituitary-adrenal and gonadal axes. Neuroimmunomodulation, 2, 299–31210.1159/000097209Search in Google Scholar PubMed

Sternberg E. M. 2006. Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nature Reviews. Immunology, 6, 318–328. 10.1038/nri1810Search in Google Scholar PubMed PubMed Central

Talamás-Rohana P., Schlie-Guzmán M.A., Hernández-Ramírez V.I., Rosales-Encina J.L 1995. T-cell suppression and selective in vivo activation of TH2 subpopulation by the Entamoeba histolytica 220-kilodalton lectin. Infection and Immunity, 63, 3953–395810.1128/iai.63.10.3953-3958.1995Search in Google Scholar PubMed PubMed Central

Tecchio C., Micheletti A., M. A. Cassatella M.A. 2014. Neutrophil-derived cytokines: facts beyond expression. Frontiers in Immunology, 5, 508. 10.3389/fimmu.2014.00508Search in Google Scholar PubMed PubMed Central

Tsutsumi V., Mena-Lopez R., Anaya-Velazquez F., Martinez-Palomo A. 1984. Cellular bases of experimental amebic liver abscess formation. The American Journal of Pathology, 117, 81–91Search in Google Scholar PubMed

Ventura-Juarez J., Campos-Esparza M.R., Pacheco-Yepez J., López-Blanco J.A., Adabache-Ortíz A., Silva-Briano M., Campos-Rodríguez R. 2016. Entamoeba histolytica induces human neutrophils to form NETs. Parasite Immunology, 38, 503–509. 10.1111/pim.12332Search in Google Scholar PubMed

Ventura-Juárez J., Jarillo-Luna A., Aguilar F., Vázquez P., Fernández M., Reyes M., Rodríguez C. 2003. Human amoebic hepatic abscess: in situ interactions between trophozoites, macrophages, neutrophils and T cells. Parasite Immunology, 25, 503–51510.1111/j.1365-3024.2003.00662.xSearch in Google Scholar PubMed

Wang Y., Gao B., Xiong S. 2014. Involvement of NLRP3 inflammasome in CVB3-induced viral myocarditis. American Journal of Physiology. Heart and Circulatory Physiology, 307, H1438–47. 10.1152/ajpheart.00441.2014Search in Google Scholar PubMed

Wnorowski A., Jozwiak K. 2014. Homo- and hetero-oligomerization of β2-adrenergic receptor in receptor trafficking, signaling pathways and receptor pharmacology. Cellular Signalling, 26, 2259–2265. 10.1016/j.cellsig.2014.06.016Search in Google Scholar PubMed

Zhou J., Yan J., Liang H., Jiang J. 2014. Epinephrine enhances the response of macrophages under LPS stimulation. BioMed Research International, 2014, 254686. 10.1155/2014/254686Search in Google Scholar PubMed PubMed Central

Received: 2017-7-4
Revised: 2017-12-13
Accepted: 2018-1-17
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS