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Licensed Unlicensed Requires Authentication Published by De Gruyter April 13, 2018

Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences

Kamel Adwan, Alaa Jayousi, Sameh Abuseir, Ibrahim Abbasi, Ghaleb Adwan and Naser Jarrar
From the journal Acta Parasitologica

Abstract

Cysticercus tenuicollis is the metacestode of canine tapeworm Taenia hydatigena, which has been reported in domestic and wild ruminants and is causing veterinary and economic losses in the meat industry. This study was conducted to determine the sequence variation in the mitochondrial cytochrome c oxidase subunit 1 (coxl) gene in 20 isolates of T. hydatigena metacestodes (cysticercus tenuicollis) collected from northern West Bank in Palestine. Nine haplotypes were detected, with one prevailing (55%). The total haplotype diversity (0.705) and the total nucleotide diversity (0.0045) displayed low genetic diversity among our isolates. Haplotype analysis showed a star-shaped network with a centrally positioned common haplotype. The Tajima’s D, and Fu and Li’s statistics in cysticercus tenuicollis population of this region showed a negative value, indicating deviations from neutrality and both suggested recent population expansion for the population. The findings of this study would greatly help to implement control and preventive measures for T. hydatigena larvae infection in Palestine.

Acknowledgment

The authors are grateful to Prof. Saleh A. Naser, University of Central Florida, College of Medicine, for language revision.

References

Bandelt H.J., Forster P., Röhl A. 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, 37–4810.1093/oxfordjournals.molbev.a026036Search in Google Scholar PubMed

Boufana B., Scala A., Lahmar S., Pointing S., Craig P.S., Dessì G., et al. 2015a. A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Veterinary parasitology. 214, 67–74. 10.1016/j.vetpar.2015.08.003Search in Google Scholar

Boufana B., Lett W., Lahmar S., Griffiths A., Jenkins D.J., Buishi I., et al. 2015b. Canine echinococcosis: genetic diversity of Echinococcus granulosus sensu stricto (ss) from definitive hosts. Journal of Helminthology, 89, 689–698. 10.1017/S0022149X15000395Search in Google Scholar

Bowles J., Blair D., McManus D.P. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology, 54, 165–17310.1016/0166-6851(92)90109-WSearch in Google Scholar PubMed

Dajani Y.F., Khalaf F.H. 1981. Hydatidosis and tenuicollosis in sheep and goats of Jordan: a comparative study. Pathogens and Global Health, 75, 175–17910.1080/00034983.1981.11687425Search in Google Scholar

Hasslinger M.A., Weber-Werringhen R. 1988. Fecal surveys in pastured sheep and the occurrence of cysticercus tenuicollis in slaughtered sheep. Angewandte Parasitologie, 29, 227–234Search in Google Scholar PubMed

Librado P., Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452. 10.1093/bioinformatics/btp187Search in Google Scholar PubMed

Mekuria E., Shimelis S., Bekele J., Sheferaw D. 2013. Sheep and goats cysticercus tenuicollis prevalence and associated risk factors. African Journal of Agricultural Research, 8, 3121–3125. 10.5897/AJAR2012.7361Search in Google Scholar

Murrell KD. (Ed.). 2005. Guidelines for the surveillance, prevention and control of taeniosis/cysticercosis. Paris, France: OIE/WHO/FAO. pp. 139Search in Google Scholar

Nakao M., Sako Y., Ito A. 2003. Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 3, 159–16310.1016/S1567-1348(03)00070-4Search in Google Scholar

Nimbalkar R.K., Shinde S.S., Kamitikar V.N., Muley S.P. 2011. Study on Taenia hydatigena in the slaughtered sheep (Ovis bharal) and goats (Capra hircus) in Maharashtra, India. Global Veterinaria, 6, 374–377Search in Google Scholar

Nourani H., Pirali Kheirabadi K.H., Rajabi H., Banitalebi A. 2010. An unusual migration of Taenia hydatigena larvae in a lamb. Tropical Biomedicine, 27, 651–656Search in Google Scholar

Oryan A., Goorgipour S., Moazeni M., Shirian S. 2012. Abattoir prevalence, organ distribution, public health and economic importance of major metacestodes in sheep, goats and cattle in Fars, southern Iran. Tropical Biomedicine, 29, 349–359Search in Google Scholar PubMed

Ramos-Onsins S.E., Rozas J. 2002. Statistical properties of new neutrality tests against population growth. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 19, 2092–210010.1093/oxfordjournals.molbev.a004034Search in Google Scholar PubMed

Rostami S., Salavati R., Beech R.N., Babaei Z., Sharbatkhori M., Baneshi M.R. et al. 2015. Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. Journal of Helminthology, 89, 150–15710.1017/S0022149X13000667Search in Google Scholar PubMed

Samuel W., Zewde, G.G. 2010. Prevalence, risk factors, and distribution of cysticercus tenuicollis in visceral organs of slaughtered sheep and goats in central Ethiopia. Tropical animal health and production. Tropical Animal Health and Production, 42, 1049–1051. 10.1007/s11250-010-9537-ySearch in Google Scholar PubMed

Scala A., Pipia A.P., Dore F., Sanna G., Tamponi C., Marrosu R., et al. 2015. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research, 114, 3137–3143. 10.1007/s00436-015-4532-xSearch in Google Scholar PubMed

Senlik B. 2008. Influence of host breed, sex and age on the prevalence and intensity of cysticercus tenuicollis in sheep. Journal of Animal and Veterinary Advances, 7, 548–551Search in Google Scholar

Soulsby E.J.L. 1982. Helminths, arthropods and protozoa of domesticated animals, 7th ed. Bailliere Tindall, London, pp. 809Search in Google Scholar

Sultan K., Desoukey A.Y., Elsiefy M.A., Elbahy N.M. 2010. An abattoir study on the prevalence of some gastrointestinal helminths of sheep in Gharbia Governorate, Egypt. Global Veterinaria, 5, 84–87Search in Google Scholar

Yamasaki H., Nakao M., Sako Y., Nakaya K., Ito A., 2005. Molecular identification of Taenia solium cysticercus genotype in the histopathological specimens. The Southeast Asian Journal of Tropical Medicine and Public Health, 36, 131–134Search in Google Scholar PubMed

Received: 2016-7-8
Revised: 2017-1-12
Accepted: 2018-1-19
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS

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