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Licensed Unlicensed Requires Authentication Published by De Gruyter April 13, 2018

First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt

Kareem Morsy, Fathy Abdel-Ghaffar, Saad Bin Dajem, Rewaida Abdel-Gaber and Fatma El Gazar
From the journal Acta Parasitologica

Abstract

Fresh muscle samples from water buffalo (Bubalus bubalis) aged 2–15, from Giza Province, Egypt; were examined for Sarcocystis infection. Macroscopic ovoid sarcocysts embedded in the muscle tissues of the examined buffaloes were detected; they measured 152–230 (210 ± 7) μm in length and 37–119 (95 ± 3) μm in width. The esophagus was the most infected organ followed by the diaphragm, and tongue, while the heart muscles were the least infected. The cyst cavity was compartmentalized by septa derived from the ground substance located under the primary cyst wall. Using transmission electron microscopy, the primary cyst wall bordered sarcocysts were determined to be 0.08–0.22 μm in thickness, raised from the parasitophorous vacuolar membrane, and surrounded by a secondary cyst wall of host origin. The primary cyst wall had irregular wall folds with numerous cauliflower-like projections of variable sizes and shapes accompanied by knob-like electron-dense elevations. 18S rRNA gene expression studies confirmed that the present parasite isolates belonged to the genus Sarcocystis. The sequence data showed significant identities (>90%) with archived gene sequences from many Eimeriidae organisms, and a dendogram showing the phylogenetic relationship was constructed. The most closely related species was Sarcocystis fusiformis KR186117, with an identity percentage of 98%. The recovered sequences were deposited in the GenBank under the accession number MG572125. The present study, to our knowledge, is the first collective ultrastructural and molecular study that confirmed the taxonomy of sarcocysts isolated from water buffaloes in Egypt as Sarcocystis fusiformis.

  1. Compliance with Ethical Standards: Parasite collection from the examined host was carried out according to the regulatory laws and ethical considerations regarding experimental ethics of animal use and collecting permits.

  2. Conflict of interest: The authors have indicated that they have no conflict of interest regarding the content of this article.

Acknowledgments

The authors would like to express their gratitude to King Khalid University, Saudi Arabia for providing administrative and technical support. Also, authors extend their appreciation to the Faculty of Science, Cairo University, Cairo, Egypt.

References

Abdel-Ghaffar F., Al-Johany A.M. 2002. A light and electron microscope study of Sarcocystis mitrani (sp. nov.) infecting the skink Scincus mitranus in the central region of Saudi Arabia. Parasitology Research, 88, 102–10610.1007/s004360100506Search in Google Scholar PubMed

Abdel-Ghaffar F., Bashtar A.R., Ashour M.B., Sakran T.H. 1990. Life cycle of Sarcocystis gongyli Trinci 1911 in the skink Chalcides ocellatus and the snake Spalerosophis diadema. Parasitology Research, 76, 444–45010.1007/BF00933555Search in Google Scholar

Abdel-Ghaffar F., Heydorn A.O., Mehlhorn H. 1989. The fine structure of cysts of Sarcocystis moulei from goats. Parasitology Research, 75, 416–41810.1007/BF00931140Search in Google Scholar PubMed

Abdel-Ghaffar F., Hilali M., Scholtyseek E. 1978. Ultrastructure study of Sarcocystis fusiformis (Railliet 1897) infecting the Indian water buffalo (Bubalus bubalis) of Egypt. Tropical Medicine and Parasitology, 29, 289–294Search in Google Scholar

Abdel-Ghaffar F., Mehlhorn H., Bashtar A.R., Al-Rasheid K., Sakran T.H., Al-Fayoumi H. 2009. Life cycle of Sarcocystis camelicanis infecting the camel (Camelus dromedarius) and the dog (Canis familiaris), light and electron microscopic study. Parasitology Research, 106, 189–19510.1007/s00436-009-1648-xSearch in Google Scholar PubMed

Abu-Elwafa S., Al-Araby M., Abbas I. 2015. Sarcocystis fusiformis infecting water buffaloes (Bubalus bubalis) in Dakahlia province, Egypt. International Journal of Advanced Research, 3, n116–120Search in Google Scholar

Ahmadi M.M., Hajimohammadi B., Eslami G., Oryan A., Yasini Ardakani S.A., Zohourtabar A., Zare S. 2015. First identification of Sarcocystis hominis in Iranian traditional hamburger. Journal of Parasitic Diseases, 39, 770–77210.1007/s12639-014-0425-7Search in Google Scholar PubMed PubMed Central

Al-Hoot A.S., Al-Qureishy S.A., Al-Rashid K., Bashtar A.R. 2005. Microscopic study on Sarcocystis moulei from sheep and goats in Saudi Arabia. Journal of the Egyptian Society of Parasitology, 35, 295–312Search in Google Scholar PubMed

Al-Quraishi S.A., Bashtar A.R., Al-Rasheid K.A.S., Abdel-Ghaffar F. 2005. The prevalence and ultrastructure of Sarcocystis species infecting camels (Camelus dromedaries) Slaughtered in Riyadh city, Saudi Arabia. Saudi Journal of Biological Sciences, 11,135–142Search in Google Scholar

Barutzki D., Schaper R. 2011. Results of parasitological examinations of faecal samples from cats and dogs in Germany between 2003 and 2010. Parasitology Research, 109, 545–56010.1007/s00436-011-2402-8Search in Google Scholar PubMed

Bashtar A.R., Abdel-Ghaffar F., El-Sayed M.A. 1988. Electron microscopic study on Sarcocystis capracanis infecting goats at Aswan area. Egyptian Journal of Histology, 11, 211–219Search in Google Scholar

Bledsoe B. 1980. Sarcocystis idahoensis sp. n. in deer mice Peromyscus maniculatus (wagner) and gopher snakes Pituophis melanoleucus (Daudin). The Journal of Protozoology, 27, 93–10210.1111/j.1550-7408.1980.tb04235.xSearch in Google Scholar PubMed

Calero-Bernal R., Cerqueira-Cézar C.K., Verma S.K., Mowery J., Carmena D., Beckmen K., Dubey J.P. 2016. Sarcocystis arctica (Apicomplexa: Sarcocystidae): ultrastructural description and its new host record, the Alaskan wolf (Canis lupus). Parasitology Research, 115, 2893–289710.1007/s00436-016-5067-5Search in Google Scholar PubMed

Černá Ž. 1984. The role of birds as definitive hosts and intermediate hosts of heteroxenous coccidians. The Journal of Protozoology, 31, 579–58110.1111/j.1550-7408.1984.tb05508.xSearch in Google Scholar PubMed

Cerqueira-Cezar C.K., Thompson P.C., Verma S.K., Mowery J., Calero-Bernal R., Antunes Murata F.H., et al. 2017. Morphological and molecular characterization of Sarcocystis arctica- like sarcocysts from the Arctic fox (Vulpes lagopus) from Alaska, USA. Parasitology Research, 116, 1871–187810.1007/s00436-017-5462-6Search in Google Scholar PubMed

Chen X., Wen T., Hu J., Liu T., Esch G.W., Liang Y., et al. 2017. Sarcocystis dehongensis n. sp. (Apicomplexa: Sarcocystidae) from water buffalo (Bubalus bubalis) in China. Parasitology Research, 116, 2145–215010.1007/s00436-017-5515-xSearch in Google Scholar PubMed

Collier L., Balows A., Sussman M. 1998. Sarcocystis Isospora and Cyclospora. In: Gransden W.R, editor. Topley and Welson’s:Microbiology and Microbial Infections. 9th ed. Vol. 5. New York: Oxford University Press Inc, pp. 319–326Search in Google Scholar

Dahlgren S., Gjerde B. 2007. Genetic characterization of six Sarcocystis species from reindeer (Rangifer tarand us tarand us) in Norway based on the small subunit rRNA gene. Veterinary Parasitology, 146, 204–21310.1016/j.vetpar.2007.02.023Search in Google Scholar PubMed

Daptardar M., Singh B.B., Aulakh R.S., Gill J.P 2016. Prevalence and first molecular identification of Sarcocystis species in cattle and water buffaloes in India. Acta Parasitologica, 61, 523–52810.1515/ap-2016-0069Search in Google Scholar PubMed

Dar K.H., Bhat M.M., Shafi M. 2017. Prevalence and organwise distribution of sarcocystiosis in buffaloes of Mohali, Punjab. Journal of Parasitic Diseases, 41, 318–32110.1007/s12639-016-0796-zSearch in Google Scholar PubMed PubMed Central

Dubey J.P., Lane E.P., Wilpe E., Suleman E., Reininghaus B., Verma S.K., et al. 2014. Sarcocystis cafferi n. sp. (Protozoa: Apicomplexa) from the African Buffalo (Syncerus caffer). Journal of Parasitology, 100, 817–82710.1645/13-467.1Search in Google Scholar PubMed

Dubey J.P., Moré G., van Wilpe E., Calero-Bernal R., Verma S.K., Schares G. 2016. Sarcocystis rommeli, n. sp. (Apicomplexa: Sarcocystidae) from Cattle (Bostaurus) and its Differentiation from Sarcocystis hominis. Journal of Eukaryotic Microbiology, 63, 62–6810.1111/jeu.12248Search in Google Scholar PubMed

Dubey J.P., Rosenthal B.M., Morales J.A., Alfaro A. 2006. Morphologic and genetic characterization of Sarcocystissp. from the African grey parrot, Psittacus erithacus, from Costa Rica. Acta Parasitologica, 51, 161–16810.2478/s11686-006-0026-4Search in Google Scholar

Dubey J.P., Speer C.A., Fayer R. 1989. Sarcocystosis of animals and man, 1st ed., CRC Press, Boca RatonSearch in Google Scholar

Dubey J.P., Naji N., Mowery J., Verma S., Calero-Bernal R. 2017. Identification of macroscopic sarcocysts of sarcocystis cameli from one-humped camel (camelus dromedarius) in Iraq. Journal of Parasitology, 103, 168–16910.1645/16-135Search in Google Scholar PubMed

El-Dakhly K.M., El-Nesr K.A., El-Nahass S., Hirata A., Sakai H., Yanai T. 2011. Prevalence and distribution patterns of Sarcocystis spp. In buffaloes in Beni-Suef, Egypt. Tropical Animal Health and Production, 43, 1549–155410.1007/s11250-011-9840-2Search in Google Scholar PubMed

El-Ganayni S.E. 2003. Ultrastructure of Sarcocystis fayeri infecting donkeys (Equusasinus) in El-Menia Governorate, Egypt. Journal of the Egyptian-German Society of Zoology, 42, 33–46Search in Google Scholar

Ellis T.J., Luton K., Baverstock P.R., Whitworth G., Tenter A.M., Johnson A.M. 1995. Phylogenetic relationships between Toxoplasma and Sarcocystis deduced from a comparison of 18S rDNA sequences. Parasitology, 110, 521–52810.1017/S0031182000065239Search in Google Scholar PubMed

El-Morsey A., El-Seify M., Desouky A.R., Abdel-Aziz M.M., El-Dakhly K.M., Kasem S., et al. 2015. Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt. Acta Parasitologica, 60, 691–910.1515/ap-2015-0098Search in Google Scholar PubMed

El-Morsey A., El-Seify M., Desouky A.R., Abdel-Aziz M.M., Sakai H., Yanai T. 2013. Morphologic identification of a new Sarcocystis sp. in the common moorhen (Gallinula chloropus) (Aves: Gruiformes: Rallidae) from Brolos Lake, Egypt. Parasitology Research, 113, 391–39710.1007/s00436-013-3667-xSearch in Google Scholar

Elsheikha H.M., Kennedy F.A., Murphy A.J., Soliman M., Mansfieled L.S. 2006. Sarcocystosis of Sarcocystis felis in cats. Journal of the Egyptian Society of Parasitology, 36, 1071–1079Search in Google Scholar PubMed

Entzeroth R., Chobotar B., Scholtyseck E., Nemesri L. 1985. Light and electron microscope study of Sarcocystis sp. from the fallow deer (Cervivs lama). Parasitology Research, 71, 33–3910.1007/BF00932916Search in Google Scholar

Fayer R. 1972. Gametogeny of Sarcocystis in cell culture. Science, 175, 65–6710.1126/science.175.4017.65Search in Google Scholar

Fayer R., Esposito D.H., Dubeya J.P. 2015. Human infections with Sarcocystis species. Clinical Microbiology Reviews, 28, 295–31110.1128/CMR.00113-14Search in Google Scholar PubMed

Ford G.E. 1987. Hosts of two canid genera, the red fox and the dog, as alternate vectors in the transmission of Sarcocystis tenella from sheep. Veterinary Parasitology, 26, 13–2010.1016/0304-4017(87)90072-0Search in Google Scholar PubMed

Fukuyo M., Battsetseg G., Byambaa B. 2002. Prevalence of Sarcocystis infection in horses in Mangolia. The Southeast Asian Journal of Tropical Medicine and Public Health, 33, 718–719Search in Google Scholar

Gillis K.D., Mackay R.J., Yowell C.A., Levy J.K., Greiner E.C., Dame J.B., et al. 2003. Naturally occurring sarcocystis infection in domestic cats (Felis catus). International Journal of Parasitology, 33, 877–88310.1016/S0020-7519(03)00090-0Search in Google Scholar

Gjerde B. 2013. Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. International Journal of Parasitology, 43, 579–59110.1016/j.ijpara.2013.02.004Search in Google Scholar PubMed

Gjerde B. 2016a. Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitology Research 115, 1473–149210.1007/s00436-015-4881-5Search in Google Scholar PubMed

Gjerde B. 2016b. The resurrection of a species: Sarcocystis bovifelis Heydorn et al., 1975 is distinct from the current Sarcocystis hirsuta in cattle and morphologically indistinguishable from Sarcocystis sinensis in water buffaloes. Parasitology Research, 115, 1–2110.1007/s00436-015-4785-4Search in Google Scholar PubMed

Gjerde B., Giacomelli S., Bianchi A., Bertoletti I., Mondani H., Gibelli L.R. 2017. Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy. Parasitology Research, 116, 1317–133810.1007/s00436-017-5410-5Search in Google Scholar PubMed

Gjerde B., Hilali M. 2016. Domestic cats (Feliscatus) are definitive hosts for Sarcocystis sinensis from water buffaloes (Bubalus bubalis). The Journal of Veterinary Medical Science, 78, 1217–122110.1292/jvms.16-0127Search in Google Scholar PubMed PubMed Central

Gjerde B., Hilali M., Abbas I.E. 2016. Molecular differentiation of Sarcocystis buffalonis and Sarcocystis levinei in water buffaloes (Bubalus bubalis) from Sarcocystis hirsuta and Sarcocystis cruzi in cattle (Bos taurus). Parasitology Research, 115, 2459–247110.1007/s00436-016-4998-1Search in Google Scholar PubMed

Gjerde B., Hilali M., Mawgood S.A. 2015. Molecular characterization of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt. Parasitology Research, 114, 3401–341310.1007/s00436-015-4566-0Search in Google Scholar PubMed

Grikienienė J. 1994. Sarcocystis in cattle and swine: significance for man and man’s role in its spreading. Proceedings of the scientific conference Animal husbandary and ecology, Kaunas, Lithuania, pp. 76–80Search in Google Scholar

Hall T.A. 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Search in Google Scholar

Hamidinejat H., Razi Jalali M.H., Gharibi D., Molayan P.H. 2015. Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR-RFLP. Journal of Parasitic Diseases, 39, 658–6210.1007/s12639-014-0426-6Search in Google Scholar PubMed PubMed Central

Holmdahl O.J., Mattsson M.J.G., Uggla A., Johansson K.E. 1994. The phylogeny of Neos- poracaninum and Toxoplasma gondii based on ribosomal RNA sequences. FEMS Microbiology Letters, 119, 187–19210.1111/j.1574-6968.1994.tb06887.xSearch in Google Scholar PubMed

Holmdahl O.J., Morrison D.A., Ellis J.T., Huong L.T. 1999. Evolution of ruminant Sarcocystis (Sporozoa) parasites based on small subunit rDNA sequences. Molecular Phylogenetics and Evolution, 11, 27–3710.1006/mpev.1998.0556Search in Google Scholar PubMed

Hu J.J., Huang S., Wen T., Esch G.W., Liang Y., Li H.L. 2017 Morphology, Molecular Characteristics, and Demonstration of a Definitive Host for Sarcocystis rommeli from Cattle (Bostaurus) in China. Journal of Parasitology, 103, 471–47610.1645/16-187Search in Google Scholar PubMed

Hu J.J., Wen T., Chen X.W., Liu T.T., Esch G.W., Huang S. 2016. Prevalence, Morphology, and Molecular Characterization of Sarcocystis heydorni Sarcocysts from Cattle (Bos Taurus) in China. Journal of Parasitology, 102, 545–54810.1645/16-49Search in Google Scholar PubMed

Huong L.T.T., Dubey J.P., Nikkilä T., Uggla A. 1997. Sarcocystis buffalonis n. sp. (Protozoa: Sarcocystidae) from the water buffalo (Bubalus bubalis) in Vietnam. Journal of Parasitology, 83, 471–47410.2307/3284413Search in Google Scholar

Jäkel T. 1995. Cyclic transmission of Sarcocystis gerbilliechis sp. n. by the Arabian saw scaled viber, Echiscoloratus to rodents of the sub family Gerbillinae. Journal of Parasitology, 81, 626–63110.2307/3283863Search in Google Scholar

JyothiSree C., Venu R., Samatha V., Malakondaiah P., Rayulu V.C. 2017. Prevalence and microscopic studies of Sarcocystis infection in naturally infected water buffaloes (Bubalusbubalis) of Andhra Pradesh. Journal of Parasitic Diseases, 41, 476–48210.1007/s12639-016-0832-zSearch in Google Scholar

Khalifa R.M., El-Nadi N.A., Sayed F.G., Omran E.K. 2008. Comparative morphological studies on three Sarcocystis species in Sohag, Journal of the Egyptian Society of Parasitology, 38, 599–608Search in Google Scholar PubMed

Latif B., Kannan Kutty M., Muslim A., Hussaini J., Omar E., Heo C.C., et al. 2015. Light microscopy and molecular identification of Sarcocystis spp. in meat producing animals in Selangor, Malaysia. Tropical Biomedicine, 32, 444–452Search in Google Scholar PubMed

Li Q.Q., Yang Z.Q., Zuo Y.X., Attwood S.W., Chen X.W., Zhang Y.P. 2002. A PCR-based RFLP analysis of Sarcocystiscruzi (Protozoa: Sarcocystidae) in Yunnan province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host. Journal of Parasitology, 88, 1259–126110.1645/0022-3395(2002)088[1259:APBRAO]2.0.CO;2Search in Google Scholar

Lukesová D., Nevole M., Haidová B. 1986. The extent of the incidence of sarcocystosis in cattle and pig farms. Veterinary Medicine, 31, 521–530Search in Google Scholar

Matuschka F.R. 1986. Sarcocystis clethrionomyelaphis n. sp. from snakes of the genus Elaphe and defferent voles of the family Arvicolidae. Journal of Parasitology, 72, 226–23110.2307/3281596Search in Google Scholar

Matuschka F.R., Bannert B. 1989. Recognition of cyclic transmission of Sarcocystis stehlinii n. sp. in the Gran Canarian giant lizard. Journal of Parasitology, 75, 383–38710.2307/3282593Search in Google Scholar

Matuschka F.R., Mehlhorn H., Abd-Al-Aal Z. 1987. Replacement of Besnoitia Matuschka and Häfner 1984 by Sarcocystis hoarensis. Parasitology Research, 74, 94–9610.1007/BF00534939Search in Google Scholar PubMed

Mehlhorn H. 2008. Encylopedic reference of parasitology 3rd ed. Springer, Verlag, Berlin10.1007/978-3-540-48996-2Search in Google Scholar

Mehlhorn H., Hartly W.J., Heydorn A.O. 1976. A comparative ultrastructural study of the cyst wall of 13 Sarcocystis species. Protistologica, 12, 451–467Search in Google Scholar

Mehlhom H., Heydom A.O. 1978. Sarcosporidia (Protozoa, Sporozoa): Life cycle and fine structure. Advances in Parasitology, 16, 73–9210.1016/S0065-308X(08)60572-2Search in Google Scholar

Mehlhorn H., Scholtyseck E. 1973. Elektron en mikrokopische Untersuchungen an Cystenstadien von Sarcocysti stenella aus der OesophagusMuskulatur des Schafes. Parasitology Research, 41, 291–310Search in Google Scholar

Metwally A.M., Abd-Ellah M.R., Al-Hosary A.A., Omar M.A. 2014. Microscopical and serological studies on Sarcocystis infection with first report of Sarcocystis cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt. Journal of Parasitic Diseases, 38, 378–8210.1007/s12639-013-0257-xSearch in Google Scholar

Modrý D., Koudela B., Slapeta J.R. 2000. Sarcocystis stenodactylicolubris n. sp., a new sarcosporidian coccidium with a snakegecko heteroxenous life cycle. Parasite, 7, 201–20710.1051/parasite/2000073201Search in Google Scholar PubMed

Mollenhauer H.H. 1964. Plastic embedding mixtures for use in electron microscopy. Stain Technology, 39, 111–114Search in Google Scholar PubMed

Moré G., Abrahamovich P., Jurado S., Bacigalupe D., Marin J.C., Rambeaud M., et al. 2011. Prevalence of Sarcocystis spp. in Argentinean cattle. Veterinary Parasitology, 177, 162–16510.1016/j.vetpar.2010.11.036Search in Google Scholar PubMed

Moré G., Regensburger C., Gos L., Pardini L., Verma S., Ctibor J., et al. 2016. Sarcocystis masoni, n. sp. (Apicomplexa: Sarcocystidae), and redescription of Sarcocystis aucheniae from llama (Lama glama), guanaco (Lama guanicoe) and alpaca (Vicugna pacos). Parasitology, 143, 617–62610.1017/S003118201600007XSearch in Google Scholar PubMed

Morsy K., Saleh A., Al-Ghamdi A., Abdel-Ghaffar F., Al-Rasheid K., Bashtar A.R., et al. 2011. Prevalence pattern and biology of Sarcocystis capracanis infection in the Egyptian goats: a light and ultrastructural study. Veterinary Parasitology, 181, 75–8210.1016/j.vetpar.2011.05.010Search in Google Scholar PubMed

Olias P.P., Olias L., Krücken J., Lierz M., Gruber A.D. 2011. High prevalence of Sarcocystis calchasi sporocysts in European Accipiter hawks. Veterinary Parasitology, 175, 230–23610.1016/j.vetpar.2010.10.025Search in Google Scholar PubMed

Olsen G.J., Woese C.R. 1993. Ribosomal RNA: A key to phylogeny. Federation of American Societies for Experimental Biology Journal, 1, 113–12310.1096/fasebj.7.1.8422957Search in Google Scholar PubMed

Oryan A., Moghaddar N., Gaur S.N.S. 1996. The distribution pattern of Sarcocystis sp., their transmission and pathogenesis in sheep in Fars province of Iran. Veterinary Research Communications, 20, 243–25310.1007/BF00366922Search in Google Scholar PubMed

Prakas P., Kutkiené L., Butkauskas D., Sruoga A., Zalakevicius M. 2013. Molecular and morphological investigations of Sarcocystis corvusi sp. nov. from the jackdaw (Corvusmonedula). Parasitology Research, 112, 1163–116710.1007/s00436-012-3247-5Search in Google Scholar PubMed

Rommel M., Heydorn A.O., Gruber V. 1972. Beitragezum Lebenszyklus der Sarkosporidien. I. Die Sporozyste von S. tenella in den Fazes der Katze. Berliner und Münchener tierärztliche Wochenschrift, 85, 101–105. (In German)Search in Google Scholar

Sakran T., Al-Hroub A., Ahmed A. 2013. Studies on Sarcocystis infecting domestic horse. American Journal of Research Communication, 1, 39–53Search in Google Scholar

Scholtyseck E., Mehlhorn H., Miiller B.E.G. 1974. Feinstruktur der Cyste und Cystenw and von Sarcocystis tenella, Besnoitia jellisoni, Frenkelia sp. und Toxoplasma gondii. The Journal of Protozoology, 21, 284–29410.1111/j.1550-7408.1974.tb03655.xSearch in Google Scholar PubMed

Scioscia N.P., Olmos L., Gorosábel A., Bernad L., Pedrana J., Hecker Y.P., et al. 2017. Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae). Parasitology International, 66, 214–21810.1016/j.parint.2017.01.021Search in Google Scholar PubMed

Stina S., Dahlgren S.S., Gjerde B. 2008. Molecular identification and phylogeny of six Sarcocystis species in moose, and a morphological description of three new species. Parasitology Research, 103, 93–11010.1007/s00436-008-0936-1Search in Google Scholar PubMed

Stolte M., Odening K., Quand T.S., Bengis R.G., Bockhardt I. 1998. Sarcocystis dubeyella n. sp. and Sarcocystis phacochoeri n. sp. (Protozoa: Sarcocystidae) from the warthog (Phacochoerus aethiopicus) in South Africa. Journal of Eukaryotic Microbiology, 45, 101–10410.1111/j.1550-7408.1998.tb05076.xSearch in Google Scholar

Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–159910.1093/molbev/msm092Search in Google Scholar PubMed

Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997. The Clustal-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–488210.1093/nar/25.24.4876Search in Google Scholar PubMed

Verma S.K., Lindsay D.S., Mowery J.D., Rosenthal B.M., Dubey J.P. 2017. Sarcocystis pantherophisi n. sp., from Eastern Rat Snakes (Pantherophis alleghaniensis) as Definitive Hosts and Interferon Gamma Gene Knockout Mice as Experimental Intermediate Hosts. Journal of Parasitology, 103, 547–55410.1645/17-2Search in Google Scholar

Yan W., Qian W., Li X., Wang T., Ding K., Huang T. 2013. Morphological and molecular characterization of Sarcocystis miescheriana from pigs in the central region of China. Parasitology Research, 112, 975–98010.1007/s00436-012-3219-9Search in Google Scholar PubMed

Yang Z.Q., Zuo Y.X., Yao Y.G., Chen X.W., Yang G.C., Zhang Y.P. 2001. Analysis of the 18S rRNA genes of Sarcocystis species suggests that the morphologically similar organisms from cattle and water buffalo should be considered the same species. Molecular and Biochemical Parasitology, 115, 283–28810.1016/S0166-6851(01)00283-3Search in Google Scholar PubMed

Received: 2018-1-11
Revised: 2018-1-23
Accepted: 2018-2-2
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS