Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter July 4, 2018

Morphological and molecular study of Neorhadinorhynchus nudus (Harada, 1938) (Acanthocephala: Cavisomidae) from Auxis thazard Lacepede (Perciformes: Scombridae) in the South China Sea

Liang Li, Hui-Xia Chen and Yue Yang
From the journal Acta Parasitologica

Abstract

In the present study, Neorhadinorhynchus nudus (Harada, 1938) is reported from the frigate tuna Auxis thazard (Lacepéde) (Perciformes: Scombridae), in the South China Sea for the first time. The detailed morphology of N. nudus was studied using light and scanning electron microscopy based on the newly collected material. The results showed some morphometric variability between our specimens and previous studies, including the number of hooks per longitudinal row and the size of copulatory bursa and eggs. Our SEM observations also revealed all proboscis hooks emerged from elevated round rims on proboscis surface. In addition, N. nudus was firstly characterised using molecular methods by sequencing and analysing the ribosomal ITS and mitochondrial cox1 regions. There is no nucleotide divergence found in the ITS sequences, but a low level of nucleotide variability detected in the cox1 regions (the level of intraspecific nucleotide variability being 0.75% to 2.54%). The DNA sequence data obtained herein will indeed be a useful reference for rapid and accurate species identification of Neorhadinorhynchus.

Acknowledgement

This study was supported by the Youth Top Talent Support Program of Hebei Province for Dr. Liang Li.

References

Amin O.M. 2013. Classification of the Acanthocephala. Folia Parasitologica, 60, 273–305. 10.14411/fp.2013.031Search in Google Scholar PubMed

Amin O.M., Nahhas F.M. 1994. Acanthocephala of marine fishes off Fiji Islands, with descriptions of Filisoma longcementglandus n. sp., Neorhadinorhynchus macrospinosus n. sp. (Cavisomidae), and gravid females of Rhadinorhynchus johnstoni (Rhadinorhynchidae); and keys to species of the genera Filisoma and Neorhadinorhynchus. Journal of Parasitology, 80, 768–774. 10.2307/3283256Search in Google Scholar

Amin O.M., Ha N.V. 2011. On four species of echinorhynchid acanthocephalans from marine fish in Halong Bay, Vietnam, including the description of three new species, and a key to the species of Gorgorhynchus. Parasitology Research, 109, 841–847. 10.1007/s00436-011-2310-ySearch in Google Scholar PubMed

Golvan Y.J. 1969. Systématique des acanthocéphales (Acanthocephala Rudolphi, 1801) L’Ordre des Palaeacanthocephala Meyer, 1931 La Super-famille des Echinorhynchoidea (Cobbold, 1876) Golvan et Houin, 1963. Mémoires Du Muséum National D’histoire Naturelle Nouvelle Série A, Zoologie, 57, 5–373. (In French)Search in Google Scholar

Goméz A., Serra M., Carvalho G.R., Lunt D.H. 2002. Speciation in ancient cryptic species complexes: evidence from the molecular phylogeny of Brachionus plicatilis (Rotifera). Evolution, 56, 1431–144410.1111/j.0014-3820.2002.tb01455.xSearch in Google Scholar PubMed

Harada I. 1938. Acanthocephalen aus Formosa. I. Annotationes Zoologicae Japonenses, 17, 419–427Search in Google Scholar

Hassanine R.M.E.-S. 2006. Acanthocephalans from red sea fishes. Family Cavisomidae Meyer, 1932: The seasonal cycle of Diplosentis nudus (Harada, 1938) Pichlein & Cribb, 2001 in a definitive fish host, and a comment on Sclerocollum Schmidt et Paperna, 1978. Acta Parasitologica, 51, 123–129. 10.2478/s11686-006-0019-3Search in Google Scholar

Král’ová-Hromadová I., Tietz D.F., Shinn A.P., Spakulová M. 2003. ITS rDNA sequences of Pomphorhynchus laevis (Zoega in Müller, 1776) and P. lucyi Williams & Rogers, 1984 (Acanthocephala: Palaeacanthocephala). Systematic Parasitology, 56, 141–145. 10.1023/A:1026127219358Search in Google Scholar

Li L., Chen H.-X., Amin O.M., Yang Y. 2017a. Morphological variability and molecular characterization of Pomphorhynchus zhoushanensis sp. nov. (Acanthocephala: Pomphorhynchidae), with comments on the systematic status of Pomphorhynchus Monticelli, 1905. Parasitology International, 66, 693–698. 10.1016/j.parint.2017.05.010Search in Google Scholar PubMed

Li L., Yang Y., Zhang L.-P. 2017b. Morphological and molecular study of Longicollum pagrosomi Yamaguti, 1935 (Acanthocephala: Pomphorhynchidae) from the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel) (Perciformes: Oplegnathidae) in the East China Sea, Systematic Parasitology, 94, 255–261. 10.1007/s11230-016-9689-xSearch in Google Scholar PubMed

Mordvinova T.N. 1988. Neorhadinorhynchus myctophumi (Acanthocephala), a new acanthocephalan species from Atlantic myctophid fish. Zoologicheskii Zhurnal, 67, 1411–1414. (In Russian)Search in Google Scholar

Perrot-Minnot M.-J. 2004. Larval morphology, genetic divergence, and contrasting levels of host manipulation between forms of Pomphorhynchus laevis (Acanthocephala). International Journal for Parasitology, 34, 45–54. 10.1016/j.ijpara.2003.10.005Search in Google Scholar PubMed

Tkach V.V., Lisitsyna O.I., Crossley J.L., Binh T.T., Bush S.E. 2013. Morphological and molecular differentiation of two new species of Pseudoacanthocephalus Petrochenko, 1958 (Acanthocephala: Echinorhynchidae) from amphibians and reptiles in the Philippines, with identification key for the genus. Systematic Parasitology, 85, 11–26. 10.1007/s11230-013-9409-8Search in Google Scholar PubMed

Smales L.R., Al-Hasson H.A.H., Al-Niaeem K.S., Al-Azizz S.A. 2016. A new species of Neorhadinorhynchus (Acanthocephala: Cavisomidae) from Platax teira (Ephippidae) from Iraqi marine waters. Transactions of the Royal Society of South Australia, 140, 90–95. 10.1080/03721426.2015.1115458Search in Google Scholar

Špakulová M., Perrot-Minnot M.-J., Neuhaus B. 2011. Resurrection of Pomphorhynchus tereticollis (Rudolphi, 1809) (Acanthocephala: Pomphorhynchidae) based on new morphological and molecular data. Helminthologia, 48, 268–277. 10.2478/s11687-011-0038-ySearch in Google Scholar

Wayland M.T. 2013. Morphological variation in Echinorhynchus truttae schrank, 1788 and the Echinorhynchus bothniensis Zdzitowiecki & Valtonen, 1987 species complex from freshwater fishes of northern Europe. Biodiversity Data Journal 1: e975. 10.3897/BDJ.1.e975Search in Google Scholar PubMed PubMed Central

Yamaguti S. 1939. Studies on the helminth fauna of Japan Part 29. Acanthocephala, II. Japanese Journal of Zoology, 8, 317–351Search in Google Scholar

Yamaguti S. 1963. Systema helminthum. Vol. V. Acanthocephala. Interscience Publishers, New York, London, pp. 423Search in Google Scholar

Received: 2017-12-17
Revised: 2018-03-01
Accepted: 2018-04-04
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS

Scroll Up Arrow