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Licensed Unlicensed Requires Authentication Published by De Gruyter July 4, 2018

Molecular characterization of Theileria spp. in livestock and the first report on the occurrence of Theileria sp. OT3 in Iran

Seyyed Javad Seyyed Tabaei, Adel Spotin, Ramin Pouriran, Abbas Shahbazi and Amirreza Javadi Mamaghani
From the journal Acta Parasitologica

Abstract

Theileria lestoquardi, T. ovis, and T. annulata are recognized as major causative agents for ovine and cattle theileriosis in Iran, respectively. Recently, there have been reports on the presence of Theileria spp. (Theileria sp. OT1, Theileria sp. OT3, and Theileria sp.). In this study, 37 blood samples were collected from sheep and cattle with clinically suspected Theileria infection in the Northwest of Iran. The samples were analyzed using a light microscope. DNA samples were amplified via nested-polymerase chain reaction (PCR) of 18S rRNA gene. The amplicons were digested with HpaII, following restriction fragment length polymorphism (RFLP) and sequenced to reconfirm Theileria species. The microscopic examination indicated that 4 out of 37 (10.8%) blood samples were infected with Theileria spp. Based on the nested PCR-RFLP and sequencing data, 5.4%, 13.5%, and 27% of blood samples were infected with Theileria sp. OT3, T. ovis, and T. annulata, respectively. The pairwise distance matrix of Theileria sp. OT3 showed 99.8–100% identity and 0–0.2% divergence in comparison with the registered sequences. The present study is the first report of Theileria sp. OT3 in Iran. To the evaluate evolution of Theileria spp. and providing resultant genetic data, further research with a larger sample is necessary in the region.


† Adel Spotin and Amirreza Javadi Mamaghani contributed equally to this work

  1. Conflict of interest: None declared

Acknowledgments

This study was financially supported by the Department of Parasitology & Mycology, Shahid Beheshti University of Medical Sciences, and Tehran, Iran.

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Received: 2017-07-13
Revised: 2018-01-31
Accepted: 2018-04-11
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS

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