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Licensed Unlicensed Requires Authentication Published by De Gruyter July 4, 2018

Molecular phylogenetic position of Haplometroides intercaecalis (Digenea, Plagiorchiidae)

Maria Isabel Müller, Drausio Honorio Morais and Reinaldo José da Silva
From the journal Acta Parasitologica


Three valid species of Haplometroides Odhner, 1910 parasitise snakes and amphisbaenians from South America. This study provides additional data on morphometric and molecular phylogenetic position inferred from the nuclear ribosomal gene 28S (partial). DNA sequences were isolated from Haplometroides intercaecalisSilva, Ferreira and Strüssmann, 2007 found in one specimen of Phalotris matogrossensisLema, D’Agostini and Cappellari, 2005. Five digenean specimens were recovered from the esophagus of this snake, and four specimens were used for morphometrical studies and one specimen for molecular analysis. Phylogenetic analysis using maximum likelihood and Bayesian methods was conducted with sequences available for the order Plagiorchiida and its phylogenetic position places H. intercaecalis among the brachycoeliids Brachycoelium (Dujardin, 1845) Stiles and Hassall, 1898 and Parabrachycoelium Pérez-Ponce de León, Mendoza-Garfias, Razo-Mendivil and Parra-Olea, 2011, and the mesocoeliid Mesocoelium Odhner, 1910, not closely related to plagiorchids as expected. Due to morphological differences among these families, it may be necessary to create a new family to accommodate Haplometroides spp. However, more genera/taxa as well as other molecular markers should be added in future studies to confirm our results and resolve this matter. This is the first phylogenetic positioning of digeneans of the genus Haplometroides, contributing to the systematic analysis of the helminthological biodiversity of Neotropical snakes.


We would like to acknowledge colleagues from the laboratory who helped us with fieldwork and Companhia Energética de São Paulo (CESP) for logistical support and authorization to work in RPPN Foz do Rio Aguapeí. We also thank the anonymous reviewers for their valuable help improving the manuscript. Financial support for this study was provided by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). M.I.M. is supported by CAPES (grant number AUX-PE-PNPD 3005/2010) and the Young Researcher Program (PROPE-UNESP 02/2016). D.H.M is supported by CAPES/PNPD #22005013001P4. R.J.S. is supported by CNPq (309125/2017-0), CNPq-PROTAX (440496/2015-2), and FAPESP 2016/50377-1.


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Received: 2018-03-05
Revised: 2018-04-10
Accepted: 2018-04-12
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS

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