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Licensed Unlicensed Requires Authentication Published by De Gruyter July 4, 2018

First molecular evidence of Thelohanellus wallagoiSarkar, 1985 (Myxozoa) from economically important food fish, freshwater shark Wallago attu (Siluridae) in India

Abhishek Gupta, Anshu Chaudhary, Anupma Garg, Chandni Verma, Hridaya S. Singh and Bindu Sharma
From the journal Acta Parasitologica


The freshwater shark Wallago attu (Bl. and Schn.) is a frequent silurid in the River Ganga and one of the most commercially exploit fish in India. In a survey on its infection with myxosporeans, spore type belongs to Thelohanellus species was found in the gills, kidney and intestine respectively. Through morphological and molecular investigations, we identified the spore as Thelohanellus wallagoiSarkar, 1985. They were pyriform in valvular view and slim in sutural view, and had one pyriform polar capsule with four to five turns. The spores measured 8.0 × 4.0 × 2.3 μm. T. wallagoi developed in small cysts in the gill lamellae, whereas cyst and scattered spores of T. wallagoi were also found in the kidney and intestine respectively. The 18S rDNA sequence of T. wallagoi isolates recovered from gills, kidney and intestine were found similar to each other and differed from any other Thelohanellus species available in GenBank and validated its status after 32 years of original description. Phylogenetic analysis signified that T. wallagoi was placed sister to Myxobolus species in the clade that indicated the polyphyletic nature of the genus Thelohanellus.


The authors thank to the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities. The authors declare that they have no conflict of interest.


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Received: 2018-01-03
Revised: 2018-04-11
Accepted: 2018-04-12
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS