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Licensed Unlicensed Requires Authentication Published by De Gruyter July 4, 2018

Dirofilaria repens infection as a cause of intensive peripheral microfilariemia in a Polish patient: process description and cases review

Matylda Kłudkowska, Łukasz Pielok, Krystyna Frąckowiak, Aleksander Masny, Elżbieta Gołąb and Małgorzata Paul
From the journal Acta Parasitologica

Abstract

Dirofilariasis is a parasitic disease of dogs and other carnivores transmitted mainly by the mosquitoes of the genera Culex, Aedes, Anopheles. Full life cycle of the Dirofilaria nematodes in humans is extremely rarely observed, usually lacking species determination at the molecular level. We report fully documented unusual clinical manifestation of subcutaneous dirofilariasis with intensive microfilariemia in peripheral blood revealed by the Knott’s concentration technique. The identification of the Dirofilaria repens nematode was based on typical morphological findings for adult gravid female nematode found in the histopathological preparations. The morphology of microfilariae obtained from patient’s peripheral blood was also typical for D. repens. The final identification was confirmed by the molecular analysis of microfilariae collected from the blood.

Acknowledgments

The authors cordially thank Katarzyna Iwanik from Heliodor Święcicki Clinical Hospital, Poznan, Poland for the professional histopathological evaluation of the preparations and Zbigniew S. Pawłowski from University of Medical Sciences, Poznan, Poland for his valuable comments to the manuscript.

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Received: 2017-12-22
Revised: 2018-03-05
Accepted: 2018-04-06
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS