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Licensed Unlicensed Requires Authentication Published by De Gruyter October 18, 2018

First report of molecular characterization and sequence phylogenetic analysis of Linguatula serrata from India

Vikrant Sudan, Daya Shanker and Amit Kumar Jaiswal
From the journal Acta Parasitologica

Abstract

Linguatula serrata is a pentastomid which is worldwide in distribution. However, a very few references are reported from India. In the present study, the cox I gene of L. serrata nymphs, originally isolated from mesenteric lymph nodes of buffaloes, was amplified and custom sequenced. Based on sequence analysis, two haplotypes were noticed and were subsequently submitted in NCBI database. The sequences were also compared with the other sequences available in the pubmed and phylogenetic analysis coupled with nucleotide homologies were commutated. The studied Indian isolates were found closer to Bangladesh and Iran isolates. This is the first report of molecular characterization of L. serrata from India.

  1. Conflict of interest. The authors declare that they have no conflict of interest.

Acknowledgement.

The authors are highly thankful to the Incharge, Central Instrumentation Facility (CIF), Dean CoVSc and Vice Chancellor, DUVASU, for the facilities provided. The authors also want to acknowledge the various funding agencies like Indian Council of Agricultural Research (ICAR) and Rashtriya Krishi Vikas Yojana (RKVY) for sanctioning various projects to the University for the procurement of instruments to carry out such work. The authors are also thankful to Dr. Sankar Muthu, Senior Scientist, IVRI (Mukteshwar Campus) for help in preparation of phylogenetic tree and its analysis.

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Received: 2018-02-22
Revised: 2018-06-20
Accepted: 2018-08-06
Published Online: 2018-10-18
Published in Print: 2018-12-19

© W. Stefański Institute of Parasitology, PAS