Abstract
Linguatula serrata is a pentastomid which is worldwide in distribution. However, a very few references are reported from India. In the present study, the cox I gene of L. serrata nymphs, originally isolated from mesenteric lymph nodes of buffaloes, was amplified and custom sequenced. Based on sequence analysis, two haplotypes were noticed and were subsequently submitted in NCBI database. The sequences were also compared with the other sequences available in the pubmed and phylogenetic analysis coupled with nucleotide homologies were commutated. The studied Indian isolates were found closer to Bangladesh and Iran isolates. This is the first report of molecular characterization of L. serrata from India.
Conflict of interest. The authors declare that they have no conflict of interest.
Acknowledgement.
The authors are highly thankful to the Incharge, Central Instrumentation Facility (CIF), Dean CoVSc and Vice Chancellor, DUVASU, for the facilities provided. The authors also want to acknowledge the various funding agencies like Indian Council of Agricultural Research (ICAR) and Rashtriya Krishi Vikas Yojana (RKVY) for sanctioning various projects to the University for the procurement of instruments to carry out such work. The authors are also thankful to Dr. Sankar Muthu, Senior Scientist, IVRI (Mukteshwar Campus) for help in preparation of phylogenetic tree and its analysis.
References
Christoffersen M.L., De Assis J.E., 2013. A systematic monograph of the recent Pentastomida, with a compilation of their hosts. Zoologische Mededeelingen, 87, 1–206. ISSN0024-0672, ISBN 978-90-6519-004-8Search in Google Scholar
Eslami G., Khalatbari-Limaki S., Ehrampoush M.H., Gholamrezaei M., Hajimohammadi B., Oryan A., 2017. Comparison of three different DNA extraction methods for Linguatula serrata as a food born pathogen.Iranian Journal of Parasitology, 12, 236– 242. PMID: 28761484Search in Google Scholar
Gjerde B., 2013. Phylogenetic position of Linguatula arctica and Linguatula serrata (Pentastomida) as inferred from the nuclear 18S rRNA gene and the mitochondrial cytochrome c oxidase subunit I gene. Parasitology Research, 112, 3517–3525. 10.1007/s00436-013-3534-9Search in Google Scholar PubMed
Gomez-Puerta L.A., Pacheco J.I., Angulo-Tisoc J.M., Lopez-Urbina M.T., Gonzalez A.E., 2017. First finding of nymphal stages of Linguatula serrata in a South American camelid, a vicuña from Peru. Veterinary Parasitology, 244, 21–24. 10.1016/j.vetpar.2017.07.019Search in Google Scholar PubMed
Hajipour N., Tavassoli M., Eslambo A.A., Javadi S.H., 2018. Investigation of histopathological changes caused by adult stage of Linguatula serrata in dog. Comparative Clinical Pathology, 27, 717–720. DOI.org/10.1007/s00580-018-2656-y10.1007/s00580-018-2656-ySearch in Google Scholar
Moller O.S., Olesen J., Avenant-Oldewage A., Thomsen P.F., Glenner H., 2008. First maxillae suction discs in Branchiura (Crustacea): development and evolution in light of the first molecular phylogeny of Branchiura Pentastomida, and other Maxillopoda. Arthropod Structure and Devlopment, 37, 333– 346. 10.1016/j.asd.2007.12.002Search in Google Scholar PubMed
Ravindran R., Lakshmanan B., Ravishankar C., Subramanian H., 2008. Prevalence of Linguatula serrata in domestic ruminants inSouth India, Southeast. Asian Journal of Tropical Medicine and Public Health, 39,808–812. PMID: 19058572Search in Google Scholar
Sivakumar P., Shankar M., Nambi P.A., Praveena P.E., Singh N., 2005. The occurrence of nymphal stage of Linguatula serrata in water buffaloes (Bubalus bubalis): nymphal morphometry and lymph node pathology. Journal of Veterinary Medicine Series A, 52, 506–509. 10.1111/j.1439-0442.2005.00774.xSearch in Google Scholar PubMed
Shamsi S., McSpadden K., Baker S., Jenkins D,J., 2017. Occurrence of tongue worm, Linguatula cf. serrata (Pentastomida: Linguatulidae) in wild canids and livestock in south-eastern Australia. International Journal for Parasitology: Parasites and Wildlife, 6, 271–277. 10.1016/j.ijppaw.2017.08.008Search in Google Scholar PubMed PubMed Central
Sudan V., Jaiswal A.K., Shanker D., 2014. Infection rates of Linguatula serrata nymphs in mesenteric lymph nodes from water buffaloes in North India. Veterinary Parasitology, 408– 411. 10.1016/j.vetpar.2014.07.025Search in Google Scholar PubMed
© W. Stefański Institute of Parasitology, PAS
