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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access January 29, 2016

Optimization of pre-treatment of de-oiled oil seed cake for release of reducing sugars by response surface methodology

  • N Sharmada , Apoorva Punja , Sonali S Shetty , Vinayaka B Shet , Louella Concepta Goveas and C Vaman Rao
From the journal Bioethanol

Abstract

Pre-treatment is a process that releases simple sugars from complex lignocellulosic biomass by using chemicals like acid and alkali which are one of the simplest and cost effective techniques. In this study, the conditions for sulphuric acid and sodium hydroxide pretreatment of de-oiled oil seed cake (DOSC) were optimized by response surface methodology (RSM). The levels of factors (DOSC concentration, agitation speed, sulphuric acid (H2SO4), sodium hydroxide (NaOH) concentration and reaction time) that affect release of reducing sugars by pre-treatment were obtained by one factor at a time (OFAT) approach of which only H2SO4 concentration, NaOH concentration and reaction time showed significance. The levels of factors were optimized by central composite design. Optimized conditions were found to be 11.65% (v/v) of H2SO4 concentration at 1.28h and, 4 N of NaOH at 3.7 h for acid and alkali hydrolysis respectively. Under optimized conditions, the release of reducing sugars was found to be 0.69 g/L (41.36 mg RRS/ g cellulose) and 0.40 g/L (23.98 mg RRS/ g cellulose) for acid hydrolysis and alkali hydrolysis of DOSC, respectively. Hence, RSM was found to be an efficient technique to optimize the hydrolysis process and ensure maximum release of reducing sugars.

References

[1] Romani A., Garrote G., Parajo J.G, Bioethanol production from autohydrolyzed Eucalyptus globules by simultaneous saccharification and fermentation operating at high solids loading, Fuel, 2012, 94, 305 –312. 10.1016/j.fuel.2011.12.013Search in Google Scholar

[2] Singh R.N., Vyas D.K., Srivastava N.S.L., Narr M., SPRERI experience on holistic approach to utilize all parts of Jatropha curcass fruit for energy. Renew Energ., 2008, 33, 1868 – 1873. 10.1016/j.renene.2007.10.007Search in Google Scholar

[3] Razmovski R., Vucurovic V., Bioethanol production from sugar beet molasses and thick juice using Saccharomyces cerevisiae immobilized on maize stem ground tissue. Fuel, 2012, 92, 1 – 8. 10.1016/j.fuel.2011.07.046Search in Google Scholar

[4] Phadatare A.G., Raheman H., Diesel engine emissions and performance from blends of Karanja methyl ester and diesel. Biomass Bioenerg., 2004, 27(4), 393-397. 10.1016/j.biombioe.2004.03.002Search in Google Scholar

[5] Dawson L., Boopathy R., Cellulosic ethanol production from sugarcane bagasse without enzymatic saccharification. Bio. Resour., 2008, 3, 452 – 460. Search in Google Scholar

[6] Kirk O. Encyclopedia of Chemical Technology. Concise. 4th ed. Wiley Interscience, 2001. Search in Google Scholar

[7] Mosier N., Wyman C., Dale B., Elander R., Lee Y.Y., Holtzapple M., et al, Features of promising technologies for pre-treatment of lignocellulosic biomass. Bioresour. Technol. 2005, 96(6), 673 – 686. Search in Google Scholar

[8] Chandel A.K., Singh O.M., Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of bio fuels. Appl. Microbiol. Biotechnol. 2011, 89(5), 1289 – 1303. Search in Google Scholar

[9] Chang V.S., Holtzapple M.T., Fundamental factors affecting biomass enzymatic reactivity. Appl. Biochem. Biotechnol. 2002, 84-86, 5 – 37. Search in Google Scholar

[10] Cheng K.K., Cai B.Y., Zhang J.A., Ling H.Z., Zhou Y.J., Ge J.P., et al, Sugarcane bagasse hemicellulose hydrolysate for ethanol production by acid recovery process. Biochem. Eng. J. 2008, 38, 105–109. Search in Google Scholar

[11] Schell D.J., Farmer J., Newman M., McMillan J.D., Dilute sulphuric acid pre-treatment of corn-stover in pilot-scale reactors. Appl. Biochem. Biotechnol. 2003, 05–108, 69–85. Search in Google Scholar

[12] Wayman, C.E., Ethanol from lignocellulosic biomass: Technology, economics and opportunities. Bioresour. Technol. 1994, 101, 2900 – 2903. Search in Google Scholar

[13] E., Hahn-Hagerdal, B., Fermentation of lignocellulosic hydrolysates. I: Inhibition and detoxification, Bioresour. Technol. 2000, 74, 17 - 24. Search in Google Scholar

[14] Rivers D.B., Zoldak B.R., Evans R.S., Emert G.H., Determination of cellulose in municipal solid wastes contaminated with synthetic material, Biotechnol. Lett., 1983, 5, 777–780. 10.1007/BF01386501Search in Google Scholar

[15] Ververis C., Georghiou K., Danielidis D., Hatzinikolaou D.G., Santas P., Santas R., Corleti V., Cellulose, hemicelluloses, lignin and ash content of some organic materials and their suitability for use as paper pulp supplements, Biores. Technol., 2007, 98, 296–301. 10.1016/j.biortech.2006.01.007Search in Google Scholar PubMed

[16] Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 1959, 31(3), 426 – 428 10.1021/ac60147a030Search in Google Scholar

[17] Statsoft (1999) Statistics for Windows, Statsoft Inc., Tulsa, USA. C. Search in Google Scholar

[18] Brummer V., Jurena T., Hlavacek V., Omelkova J., Bebar L., Gabriel P., Stehlik P., Enzymatic hydrolysis of pretreated waste paper – Source of raw material for production of liquid biofuels, Biores. Technol., 2014, 152, 543 – 547. 10.1016/j.biortech.2013.11.030Search in Google Scholar PubMed

[19] Rajan K., Carrier D.J., Effect of dilute acid pre-treatment conditions and washing on the production of inhibitors and on recovery of sugars during wheat straw enzymatic hydrolysis, Biomass Bioenerg., 2014, 62, 222 – 227 10.1016/j.biombioe.2014.01.013Search in Google Scholar

[20] Manzoor A., Khokhar Z., Hussain A., Uzma, Ahmad S.H., Syed Q., Baig S., Dilute sulphuric acid: a cheap acid for optimization of bagasse pre-treatment, Sci. Int.(Lahore), 2012, 24(1), 41 – 45 Search in Google Scholar

[21] Kuttiraja M., Sindhu R., Varghese P.E., Sandhya S.V., Binod P., Vani S., et al., Bioethanol production from bamboo (Dendrocalamus sp.) process waste, Biomass Bioenerg., 2013, 59, 142 – 150 10.1016/j.biombioe.2013.10.015Search in Google Scholar

[22] Amenaghawon A.N., Balogun A.A., Agbonghae E.E., Ogbeide S.E., Okieimen C.O., Statistical optimisation of dilute acid pre-treatment of corn stover using response surface methodology, J. Environment, 2013, 2(2), 34 – 40 Search in Google Scholar

[23] Sukri S.S.M., Rahman R.S., Illias R.M., Yaakob H., Optimization of alkaline pre-treatment conditions of oil palm fronds in improving the lignocelluloses contents for reducing sugar production, Romanian Biotechnol. Lett., 2014, 19(1), 9006 – 9018 Search in Google Scholar

Received: 2014-11-12
Accepted: 2015-7-28
Published Online: 2016-1-29

© 2016 Sharmada N et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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