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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access April 2, 2015

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

  • Anca Hermenean , Miruna Stan , Aurel Ardelean , Luminița Pilat , Ciprian Valentin Mihali , Cristina Popescu , Lajos Nagy , György Deák , Miklós Zsuga , Sándor Kéki , Ildikó Bácskay , Ferenc Fenyvesi , Marieta Costache , Anca Dinischiotu and Miklós Vecsernyés
From the journal Open Life Sciences


This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.


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Received: 2014-3-4
Accepted: 2014-8-20
Published Online: 2015-4-2

©2015 Anca Hermenean et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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