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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 14, 2015

Antifungal activity of some botanical extracts on Fusarium oxysporum

  • Domenico Rongai , Patrizio Pulcini , Barbara Pesce and Filomena Milano
From the journal Open Life Sciences


The present paper describes the antifungal activity of some plant extracts on the development of Fusarium oxysporum f.sp. lycopersici. The best extracts were selected to be tested as a phytofungicide to control crop diseases, with the ultimate goal of developing a green alternative to synthetic fungicides. Using the conidia germination assay, of the 24 plant extracts tested, 15 reduced conidia germination and 6 completely inhibited germination. Extracts of Rivina humulis, Brassica carinata, Brunfelsia calyicina, Salvia guaranitica and Punica granatum showed good antifungal activity. The relationship between total phenolic content (TPC) in each plant extract tested and the percentage of mycelial growth inhibition showed a significant correlation (R2 = 0.69), while no correlation was found between total flavonoid content (TFC) and percentage mycelial growth inhibition. Among all extracts tested, Punica granatum and Salvia guaranitica showed the best inhibitory effect against Fusarium oxysporum f.sp. lycopersici . Our results indicate that plant extracts with a good antifungal activity generally had a high level of total polyphenolic content and titratable acidity, and low values of pH.


[1] Tegegne G., Pretoriusa J.C., Swart W.J., Antifungal properties of Agapanthus africanus L. extracts against plant pathogens, Crop Protection, 2008, 27, 1052-1060. 10.1016/j.cropro.2007.12.007Search in Google Scholar

[2] Lira S.R.H., Balvantin G.G.F., Hernández C.F.D., Gamboa A.R., Jasso de Rodríguez D., Jiménez D.F., Evaluation of resin content and the antifungal effect of Larrea tridentate (Sesse and Moc. Ex D.C.) Coville extracts from two Mexican deserts against Pythium sp. Pringsh, Mexican J. Phytopath., 2003, 21, 97-101. Search in Google Scholar

[3] Osorio E., Flores M., Hernández D., Ventura J. Rodríguez R., Aguilar C.N., Biological efficiency of polyphenolic extracts from pecan nuts shell (Carya illinoensis), pomegranate usk (Punica granatum) and creosote bush leaves (Larrea tridentate Cov.) against plant pathogenic fungi, Ind. Crops Products, 2010, 31, 153-157. 10.1016/j.indcrop.2009.09.017Search in Google Scholar

[4] Pârvu M., Pârvu A.E., Vlase L., Roşca-Casian O., Pârvu O., Antifungal properties of Allium ursinum L. ethanol extract, J. Med. Plant Research, 2011, 5, 2041-2046. Search in Google Scholar

[5] Siva N., Ganesan S., Banumathy N., Muthuchelian K., Antifungal Effect of Leaf Extract of Some Medicinal Plant Against Fusarium oxysporum Causing Wilt Disease of Solanum melogena L., Ethnobotanical Leaflets, 2008, 12, 156-163. Search in Google Scholar

[6] Singha I.M., Kakoty Y., Unni B.G., Kalita M.C., Das J., Naglot A., Wann S.B., Singh L., Control of Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici using leaf extract of Piper betle L.: a preliminary study, World J. Microbiol. Biotechnol., 2011, 27, 2583-2589. 10.1007/s11274-011-0730-6Search in Google Scholar

[7] LalithaV., Kiran B., Raveesha K.A., Antifungal and antibacterial potentiality of six essential oils extracted from plant source, Int. J. Eng. Sci. Techn., 2011, 3, 3029-3038. Search in Google Scholar

[8] Minz S., Samuel C.O.,& Tripathi S.C., The effect of Plant Extract on the Growth of Wilt Causing Fungi Fusarium oxysporum, J. Pharm. Bio. Sci., 2012, 4, 13-16. 10.9790/3008-0411316Search in Google Scholar

[9] Rongai D., Milano F., Sciò E., Inhibitory Effect of Plant Extracts on Conidial Germination of the Phytopathogenic Fungus Fusarium oxysporum, Am. J. Plant Science, 2012, 3, 1693-1698. 10.4236/ajps.2012.312207Search in Google Scholar

[10] Ribera A., Cotoras M., Zúñiga G. E., Effect of extracts from in vitro-grown shots of Quillaja saponaria Mol. on Botrytis cinerea Pers, World J. Microbiol. Biotechnol., 2008, 24, 1803-1811. 10.1007/s11274-008-9673-ySearch in Google Scholar

[11] Stanković M.S., Stefanović O., Čomić L., Topuzović M., Radojević I., Solujić S., Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species, Cent. Eur. J. Biol., 2012, 7, 664-671. 10.2478/s11535-012-0048-xSearch in Google Scholar

[12] Anis Z., Sulaiman O., Hashim R., Mehdi S.H., Ghalib R.M., Radical scavenging activity, total phenol content and antifungal activity of Cinnamomum iners Wood, Iranica J. Energy & Environ., 2012, 3, 74-78. 10.5829/idosi.ijee.2012.03.05.12Search in Google Scholar

[13] Mahboubia M., Khamechianb T., Kazempoura N., Total Phenolic and Flavonoids Content, Antioxidant and Antimicrobial Activities of Azilia eryngioides Extracts, J. Biol. Active Prod. Nat., 2012, 2, 144-150. 10.1080/22311866.2012.10719121Search in Google Scholar

[14] El-Khateeb A.Y., Elsherbiny E.A., Tadros L.K., Ali S.M., Hamed H.B., Phytochemical analysis and antifungal activity of fruit leaves extracts on the mycelial growth of fungal plant pathogens, J. Plant Pathol. Microbiol., 2013, 4, 199. doi:10.4172/2157-7471.1000199. 10.4172/2157-7471.1000199Search in Google Scholar

[15] Ruan Y., Kotraiah V., Straney D.C., Flavonoids stimulate spore germination in Fusarium solani pathogenic on legumes in a manner sensitive to inhibitors of cAMP-dependent protein kinase, Mol. Plant Micr. Interactions, 1995, 8, 929-938. 10.1094/MPMI-8-0929Search in Google Scholar

[16] Rhouma A., Daoud H.B., Ghanmi S., Salah H.B., Romdhane M., Demak M., Antimicrobial activities of leaf extracts of Pistacia and Schinus species against some plant pathogenic fungi and bacteria, J. Plant Path., 2009, 91, 339-345. Search in Google Scholar

[17] El Hadrami A., Adam L.R., Daayf F., Biocontrol treatments confer protection against Verticillium dahliae infection in potato by inducing anti-microbial metabolites, Mol. Plant Microbe Interact., 2011, 24, 328–335. 10.1094/MPMI-04-10-0098Search in Google Scholar PubMed

[18] Slinkard K., Singleton V.L., Total phenol analyses: Automation and Comparison with Manual Methods, Am. J. Enol. Vitic., 1977, 28, 49-55. Search in Google Scholar

[19] Nongalleima K., Dey A., Deb L., Singh C.B., Thongam B., Devi H.S., Devi S.I., Endophytic Fungus Isolated From Zingiber zerumbet (L.) Sm. Inhibits Free Radicals And Cyclooxygenase Activity, Int. J. Pharm. Tech. Res., 2013, 5, 301-307. Search in Google Scholar

[20] Avita E.J., Phytochemical screening and bioactivity assay of selected South Indian phytolaccaceae, J. Nat. Life Sci., 2013, 1, 26-30. Search in Google Scholar

[21] Carrer R.P., Vanderlinde R., Dutra S., Marcon A., Echeverrigaray S., Essential oil variotion among Brazilian accessions of Salvia guaranitica L., Flavour Fragr. J., 2007, 22, 430-435. 10.1002/ffj.1817Search in Google Scholar

[22] Abdelgaleil S.A.M., Badawy M.E.I., SuganumaT., Kitahara K., Antifungal and biochemical effects of pseudoguaianolide sesquiterpenes isolated from Ambrosia maritima L., Afr. J. Microbiol. Res., 2011, 5, 3385-3392. 10.5897/AJMR11.364Search in Google Scholar

[23] Ifesan B.O.T., Fashakin J.F., Ebosele F., Oyerinde A.S., Antioxidant and Antimicrobial Properties of Selected Plant Leaves., Eur. J. Med. Plants, 2013, 3, 465-473. 10.9734/EJMP/2013/3383Search in Google Scholar

[24] Orak H. H., Demi̇rci̇ A. Ş., Gümüş T., Antibacterial and antifungal activity of pomegranate (Punica granatum L. cv.) peel, Electron. J. Environ. Agric. Food Chem., 2011, 10, 1958-1969. Search in Google Scholar

[25] Oliveira C.E.V., Stamford T.L.M., Neto N.J.G., Souza E.L., Inhibition of Staphylococcus aureus in broth and meat broth using synergies of phenolics and organic acids, Int. J. Food Microbiol., 2010, 137, 312-316. 10.1016/j.ijfoodmicro.2009.11.019Search in Google Scholar PubMed

[26] Pimia R.P., Nohynek L., Schmidlin S.H., Kahkonen M., Heinonen M., Riihinen K.M., Caldentey O.K.M., Berry phenolics selectively inhibit the growth of intestinal pathogens, J. Appl. Microbiol., 2005, 98, 991–1000. 10.1111/j.1365-2672.2005.02547.xSearch in Google Scholar PubMed

Received: 2014-3-5
Accepted: 2014-10-13
Published Online: 2015-9-14

©2015 Domenico Rongai, et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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