Identification of circRNA circ-CSPP1 as a potent driver of colorectal cancer by directly targeting the miR-431/LASP1 axis

Abstract Colorectal cancer (CRC) is the third most common malignancy worldwide. Circular RNAs (circRNAs) have been implicated in cancer biology. The purpose of the current work is to investigate the precise parts of circRNA centrosome and spindle pole-associated protein 1 (circ-CSPP1) in the progression of CRC. Our data showed that circ-CSPP1 was significantly overexpressed in CRC tissues and cells. The knockdown of circ-CSPP1 attenuated cell proliferation, migration, invasion and promoted apoptosis in vitro and weakened tumor growth in vivo. circ-CSPP1 directly targeted miR-431, and circ-CSPP1 knockdown modulated CRC cell progression in vitro via upregulating miR-431. Moreover, LIM and SH3 protein 1 (LASP1) was a functional target of miR-431 in modulating CRC cell malignant progression. Furthermore, circ-CSPP1 in CRC cells functioned as a posttranscriptional regulator on LASP1 expression by targeting miR-431. Our present study identified the oncogenic role of circ-CSPP1 in CRC partially by the modulation of the miR-431/LASP1 axis, providing evidence for circ-CSPP1 as a promising biomarker for CRC management.


Introduction
Colorectal cancer (CRC) is the third most prevalent malignancy worldwide as of 2018, accounting for approximately one in ten cancer cases and deaths [1]. Although new treatment options have markedly increased the overall survival of non-metastasized CRC, the prognosis remains very poor for those with advanced disease [2]. Currently, biomarkers are considered as important tools in early detection and clinical therapy of patients with CRC [3]. Thus, there is an urgent need to identify novel and effective biomarkers for managing CRC.
Circular RNAs (circRNAs), mostly formed by head-totail splicing of exons, are an important family of posttranscriptional regulators in eukaryotes [4]. circRNAs are attracting considerable attention due to their microRNA (miRNA) regulation in almost all cancers, including CRC [5,6]. For instance, Yuan et al. highlighted hsa_circ_ 0026344 as a novel prognostic biomarker for CRC patients by regulating miR-21 and miR-31 [7]. Li et al. identified hsa_circ_0006990 as a negative regulator of miR-101, with the ability to accelerate CRC tumorigenesis [8]. Bian et al. illuminated that hsa_circ_103809 controlled the carcinogenesis of CRC through targeting miR-532-3p [9]. As for circRNA centrosome and spindle pole-associated protein 1 (circ-CSPP1, hsa_circ_0001806), an upregulated circRNA in CRC, it was reported to be implicated in the stemness of CRC cells through the modulation of miR-193-5p [10]. However, no insight has been gained into the precise parts of circ-CSPP1 in CRC progression. miRNAs, an evolutionarily conserved class of small RNAs, are able to silence gene expression during tumorigenesis [11]. Abnormal miRNA expression has been demonstrated in CRC [12,13]. Su and Liu uncovered that miR-431 (also called miR-431-5p) was underexpressed in CRC and functioned as a potent antitumor miRNA in this disease by directly targeting cullin-4B [14]. Nevertheless, whether miR-431 is a downstream mediator of circ-CSPP1 in controlling CRC development has not been described.
The gene expression regulation of circRNAs exhibited by miRNAs [15] led us to hypothesize that circRNA-miR-NA-mRNA networks might serve as crucial modulators of CRC development. Here, we identified a significantly overexpressed circRNA, circ-CSPP1, participated in CRC development through targeting the miR-431/LIM and SH3 protein 1 (LASP1) axis.

Clinical specimens and human study
Primary CRC tissues and paired non-tumor tissues from 37 patient volunteers were collected from the Tianjin Third Central Hospital. The clinicopathological data of these patients are provided in Table 1. A portion of the specimens was stored at −80°C and used to investigate circ-CSPP1, miR-431, and LASP1 mRNA expression levels and analyze their correlations. Also, a portion of the specimens was used for histological analysis by two pathologists using hematoxylin and eosin (H&E) staining.
Briefly, CRC samples were fixed in formaldehyde (Solarbio, Beijing, China), blocked in paraffin, and then sectioned at 4 µm intervals. The sections were stained with hematoxylin (Solarbio) for 5 min and eosin (Solarbio) for 3 min, followed by the observation of the images under a microscope (Leica, Wetzlar, Germany).
Informed consent: Informed consent has been obtained from all the individuals included in this study.
Ethical approval: The research related to human use has been complied with all the relevant national regulations, institutional policies and in accordance with the tenets of the Helsinki Declaration; and the research has been approved by the Ethics Committee of Tianjin Third Central Hospital. The plasmid construct overexpressing circ-CSPP1 was generated by cloning the overall length of hsa_circ_ 0001806 into a pCD25-ciR vector (Geneseed, Guangzhou, China), and the control plasmid (vector) was produced by using a scrambled sequence. LASP1 overexpression plasmid was created by introducing human LASP1 (NM_006148.4) into the pcDNA3.1 vector (Invitrogen, Merelbeke, Belgium) with BamH I and Xho I restriction sites. These constructs were transiently transfected into HCT-116 and SW480 cells (∼50% confluence) at 100 ng with Lipofectamine 2000 (Thermo Fisher Scientific). Lentiviruses encoding circ-CSPP1-shRNA (sh-circ-CSPP1) or nontarget-shRNA (sh-NC) were obtained from Geneseed and used as per the manufacturing instructions. circ-CSPP1 was silenced in HCT-116 and SW480 cells by transiently transfecting circ-CSPP1-siRNA (50 nM, si-circ-CSPP1, 5′-AUUUGGAUGUUU CAUUGUCUG-3′; GenePharma, Shanghai, China) or in SW480 cells by stably transducing cells with sh-circ-CSPP1. Also, miR-431 overexpression and knockdown in HCT-116 and SW480 cells were achieved by using miR-431 mimic (5′-UGUCUUGCAGGCCGUCAUGCA-3′) and matched

Subcellular fractionation
Nuclear RNA and cytoplasmic RNA were isolated from HCT-116 and SW480 cells with the Cytoplasmic & Nuclear RNA Purification Kit (Cat. NGB-37400; Norgen Biotek, Thorold, ON, Canada) as recommended by the manufacturers. RNU6 (U6) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as the controls.

Quantitative reverse transcription polymerase chain reaction (qRT-PCR)
To measure circ-CSPP1 and mRNA levels, cDNA preparation was done in 25 µL reactions using ReverTra Ace (Cat. 410800; Toyobo, Tokyo, Japan) with 1 µg total RNA and 250 ng random primers (Invitrogen); qRT-PCR analysis using SYBR qPCR Mix (Cat. QPS-201; Toyobo) plus 25 ng of both the forward and reverse primers (  (Table A1) for qRT-PCR were used as per the accompanying protocols. Using the comparative Ct method (2 −ΔΔCt with logarithm transformation) [16], the results were normalized on the basis of control GAPDH or U6 expression.

Cell viability and colony formation assays
For cell viability assay, transiently transfected HCT-116 and SW480 cells in 96-well plates at an initial density of 1 × 10 3 cells per well were cultured in DMEM growth media for 48 h, and the media was replaced with medium containing 10 µL per well of the Cell Counting Kit-8 (CCK-8) substrate (Cat. ab228554; Abcam, Cambridge, UK). Following the 37°C incubation for 2 h, Varioskan Flash (Thermo Fisher Scientific) was used for the measurement of the absorbance at 460 nm. For colony formation assay, transiently transfected HCT-116 and SW480 cells in 6-well plates at 150 cells per well were cultured in DMEM growth media. Colonies were allowed to grow for 14-21 days before imaging and counting. The number of colonies (over 50 cells) was manually counted to get an average of three fields after being stained with 0.5% crystal violet (Solarbio).

Flow cytometry
A total of 1 × 10 6 transiently transfected HCT-116 and SW480 cells were incubated with Annexin V-fluorescein isothiocyanate (BD Biosciences, North Ryde, NSW, Australia) and propidium iodide (PI; Invitrogen) for 15 min in the dark and analyzed within 1 h with a flow cytometer (BD Biosciences).

Transwell migration and invasion assays
Transiently transfected HCT-116 and SW480 cells were seeded onto the non-coated membrane (24-transwell insert, 8 µm; Corning, Rochester, NY, USA) at 2.5 × 10 4 cells per well for migration assay and plated onto the Matrigel-precoated membrane (BD Biosciences) in the insert of a 24-well plate at 5 × 10 4 for invasion assay.
Media plus 10% fetal calf serum was used as a chemoattractant in the lower chamber. After the 37°C incubation for 24 h, the migratory or invasive cells were stained with 0.5% crystal violet and counted under a 100× magnification microscope.

Bioinformatics and dual-luciferase reporter assay
The targeted miRNAs of circ-CSPP1 were predicted by online CircInteractome database. miRNA-binding sites were searched by online software starBase v.3. The genomic fragments of circ-CSPP1 and LASP1 3′-UTR encompassing the miR-431-binding region or mutated seed sequence were individually ligated into the psiCHECK-2 vector (Promega, Southampton, UK). These reporters (100 ng) were cotransfected with 25 nM of miR-431 mimic or control mimic into either HCT-116 or SW480 cells (1 × 10 5 ) in 24-well plates.
Post 48 h of incubation, luciferase assays were done using the Dual-luciferase Assay System (Promega).

Xenograft model studies
For the establishment of the xenograft model, 6-to 8-week-old BALB/c female mice (n = 12; Vital River Laboratory, Beijing, China) were used. Also, 2 × 10 6 sh-NC-or sh-circ-CSPP1-transduced SW480 cells in 200 µL of 50% Matrigel Matrix (BD Biosciences) were subcutaneously inoculated into the flanks of BALB/c mice (n = 6 per group). Tumor volume was periodically measured with a digital caliper and estimated using the (length × width 2 ) × 0.5 formula. At end points, mice were euthanized to harvest the xenograft tumors.
Ethical approval: The research related to animal use has been complied with all the relevant national regulations and institutional policies for the care and use of animals; and the research has been approved by the Animal Care and Use Committee of Tianjin Third Central Hospital.

Immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay
The xenograft tumors were fixed with 4% neutral-buffered formalin, dehydrated, embedded in paraffin, and sectioned at 5 µm intervals. Proliferation and invasion of the xenograft tumors were evaluated by immunohistochemistry using anti-Ki-67 (ab21700; Abcam; dilution 1:1) and anti-MMP9 (ab228403; Abcam; dilution 1:5,000) antibodies, respectively. Briefly, paraffin-embedded sections were dewaxed, heated in 0.01 M of sodium citrate buffer, and treated with methanolic H 2 O 2 , followed by the incubation with anti-Ki-67 and anti-MMP9. Immunoreactive signals were detected using 3,3′-diaminobenzidine substrate (Thermo Fisher Scientific), and then the sections were counterstained with hematoxylin and mounted. Apoptosis of the tumor cells was assessed by staining paraffin-embedded sections with the TUNEL Assay Kit (Cat. ab206386; Abcam) as per the accompanying guidance.

Statistical analysis
All experiments were carried out in triplicate, and the results were presented as mean ± standard deviation. The Mann-Whitney U test, Student's t-test, and analysis of variance were used to compare the measured parameters.
The correlations between miR-431 and circ-CSPP1 or LASP1 expression in cancer tissues were judged by a statistic test based on Pearson's correlation coefficient. For survival data, the Kaplan-Meier method and log-rank tests were used. The values were statistically significant at *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001.

circ-CSPP1 was overexpressed in CRC tissues and cells
To observe the involvement of circ-CSPP1 in CRC development, we used qRT-PCR to determine its expression in cancer tissues and paired noncancerous tissues, which were identified by H&E staining (Figure 1a). Remarkably, circ-CSPP1 was upregulated in CRC tissues compared with normal tissues (Figure 1b). Similarly, by contrast, circ-CSPP1 level was strikingly augmented in CRC cells (Figure 1c). To evaluate the clinical significance of circ-CSPP1 in CRC, we analyzed the correlation between the overall survival of patients and circ-CSPP1 level. As expected, Kaplan-Meier survival curves revealed that the patients with low circ-CSPP1 expression had a longer survival time than those with high circ-CSPP1 level (Figure 1d). Furthermore, the expression of circ-CSPP1 was closely associated with the tumor node metastasis (TNM) grade, lymph node metastasis, and size of these tumors (Table 1). Moreover, the digestion with RNase R led to a striking reduction in the level of corresponding linear mRNA (Linear CSPP1), and circ-CSPP1 was resistant to RNase R (Figures 1e and f), demonstrating the stability of circ-CSPP1. Additionally, subcellular localization results revealed that circ-CSPP1 was mainly localized in the cytoplasm of HCT-116 and SW480 cells (Figure 1g and h).

Knockdown of circ-CSPP1 attenuated CRC cell proliferation, migration, invasion and enhanced apoptosis in vitro
We then carried out loss-of-function analyses in vitro using circ-CSPP1-siRNA (si-circ-CSPP1). Transient transfection of si-circ-CSPP1, but not the si-NC control, caused a remarkable downregulation of circ-CSPP1 level in both HCT-116 and SW480 cells (Figure 2a). In contrast, the knockdown of circ-CSPP1 led to a striking inhibition in cell viability (Figure 2b) and colony formation (Figure 2c), and a significant promotion in cell apoptosis (Figure 2d), as well as a prominent repression in cell migration ( Figure 2e) and invasion (Figure 2f). Furthermore, western blot results showed that circ-CSPP1 knockdown resulted in reduced levels of proliferation marker Ki-67, anti-apoptotic protein Bcl-2, and metastasis-related protein MMP9 in the two CRC cells (Figure 2g and h).

circ-CSPP1 directly targeted miR-431 in CRC cells
To understand the mechanism by which circ-CSPP1 regulated CRC development, we used online CircInteractome database to help identify the targeted miRNAs. Of interest, a putative miR-431 binding region was predicted within circ-CSPP1 (Figure 3a). When we cloned the circ-CSPP1 segment containing the target region into a luciferase vector, the cotransfection of the luciferase reporter (WT-circ-CSPP1) and miR-431 mimic into the two CRC cells produced lower luciferase activity than cells cotransfected with mimic control, but the mutant in the seed region (MUT-circ-CSPP1) completely abolished the downregulated impact of miR-431 mimic (Figure 3b and c). Additionally, the data of qRT-PCR showed the remarkable underexpression of miR-431 in CRC tissues and cells (Figure 3d and e). Moreover, a strong inverse correlation between miR-431 and circ-CSPP1 levels was discovered in CRC tissues (Figure 3f). The transfection efficiency of circ-CSPP1 overexpression plasmid into the two cells was gauged by qRT-PCR ( Figure 3g). Importantly, miR-431 expression was significantly increased by circ-CSPP1 knockdown and decreased when circ-CSPP1 is overexpressed in the two CRC cells (Figure 3h).

Knockdown of circ-CSPP1 regulated CRC cell progression in vitro by upregulating miR-431
We then determined whether miR-431 was a molecular mediator of circ-CSPP1 in regulating CRC cell progression in vitro. By contrast, the transfection of anti-miR-431 remarkably abrogated the augmentation of si-circ-CSPP1 on miR-431 level in both HCT-116 and SW480 cells (Figure 4a). "Rescue" experiments wherein miR-431 was underexpressed prominently abolished si-circ-CSPP1-mediated inhibition on cell viability (Figure 4b) and colony formation (Figure 4c), and promotion on cell apoptosis (Figure 4d), as well as repression on cell migration ( Figure 4e) and invasion ( Figure 4f). Furthermore, the reduced expression of miR-431 dramatically reversed the suppressive effect of circ-CSPP1 knockdown on Ki-67, Bcl-2, and MMP9 levels in the two cells (Figure 4g and h).

LASP1 in CRC cells was directly targeted and repressed by miR-431
To identify downstream effectors of miR-431, we used online target-prediction software starBase v.3 based on the presence of binding sites in the 3′-UTR. Interestingly, the predicted data revealed that LASP1 harbored a putative target sequence for miR-431 within its 3′-UTR (Figure 5a).
To confirm a direct relationship between miR-431 and LASP1, we used the LASP1 3′-UTR luciferase reporters in dual-luciferase assays. The transfection of the wild-type reporter (LASP1 3′-UTR-WT) in the presence of miR-431 mimic caused a significant downregulation of relative luciferase activity, and this effect was abrogated by the mutation of the miR-431 putative target region (LASP1 3′-UTR-MUT, Figure 5b and c). The data of qRT-PCR and western blot also revealed that LASP1 was strikingly overexpressed in CRC tissues and cells (Figure 5d-f). Moreover, in CRC tissues, LASP1 mRNA level was inversely correlated with miR-431 expression (Figure 5g). We further determined whether miR-431 modulated LASP1 expression in the two CRC cells. The transfection efficiencies of miR-431 mimic or anti-miR-431 were validated by qRT-PCR ( Figure 5h). As expected, LASP1 protein level was significantly reduced   (g and h). ***P < 0.001 or ****P < 0.0001.
by the enforced expression of miR-431, while it was remarkably augmented when miR-431 depleted in both HCT-116 and SW480 cells (Figure 5i).

LASP1 was a functional target of miR-431 in regulating CRC cell progression in vitro
In addition to the reduced effect on LASP1 expression (Figure 6a), the enforced expression of miR-431 significantly repressed cell viability (Figure 6b), colony formation (Figure 6c), and enhanced cell apoptosis (Figure 6d), as well as inhibited cell migration (Figure 6e) and invasion (Figure 6f). Moreover, miR-431 overexpression led to a striking reduction in the levels of Ki-67, Bcl-2, and MMP9 in the two CRC cells (Figure 6g and h). To provide further mechanistic insight into the link between miR-431 and LASP1 in CRC development, we elevated LASP1 level using the LASP1 overexpression plasmid in miR-431-overexpressing cells. Remarkably, the transfection of LASP1 overexpression plasmid reversed the reduction of miR-431 overexpression in LASP1 level (Figure 6a). Functional analysis results showed that the restored level of LASP1 prominently abrogated miR-431 overexpression-mediated repression on cell viability ( Figure 6b) and colony formation (Figure 6c), and enhancement on cell apoptosis (Figure 6d), as well as inhibition on cell migration (Figure 6e), invasion (Figure 6f), and Ki-67, Bcl-2, and MMP9 levels (Figures 6g  and h) in both HCT-116 and SW480 cells. More interestingly, the knockdown of circ-CSPP1 induced a striking downregulation of LASP1 protein level in the two CRC cells, and this effect was dramatically abolished by anti-miR-431 (Figure 6i), demonstrating the control of circ-CSPP1 on LASP1 expression by miR-431.

Knockdown of circ-CSPP1 attenuated tumor growth in vivo
We next asked whether circ-CSPP1 influenced tumor growth in vivo. To address this possibility, we reduced circ-CSPP1 expression in SW480 cells using a sh-circ-CSPP1 lentivirus (Figure 7a). To begin, we injected sh-NCinfected or sh-circ-CSPP1-transduced SW480 cells into the nude mice to generate the xenograft model. By contrast, the transduction of sh-circ-CSPP1 led to a significant suppression of tumor growth (Figure 7b and c). Moreover, circ-CSPP1 and LASP1 levels were remarkably downregulated and miR-431 expression was strikingly upregulated in the tumor tissues derived from the sh-circ-CSPP1transduced SW480 cells (Figure 7d and e). Additionally, immunohistochemistry results showed that Ki-67 and MMP9 levels were decreased in sh-circ-CSPP1-transduced SW480 tumor tissues (Figure 7f), demonstrating the repression of circ-CSPP1 knockdown on tumor cell proliferation and metastasis. Furthermore, circ-CSPP1 knockdown promoted SW480 cell apoptosis confirmed by TUNEL assay (Figure 7f).

Discussion
To date, multiple lines of evidence have argued for the critical involvement of circRNAs in controlling CRC development [18][19][20]. Despite these studies, the precise actions of circRNAs in CRC development have remained unclear. circ-CSPP1 was recently identified as a circRNA whose overexpression in CRC contributed to the stemness of CRC cells [10]. Our current study expanded on this by identifying circ-CSPP1 as a crucial regulator in CRC cell malignant behaviors. Several recent studies have reported the oncogenic role of circ-CSPP1 in ovarian cancer, cervical cancer, glioma, and hepatocellular carcinoma [21][22][23][24]. Here, our work showed a remarkable overexpression of circ-CSPP1 (e and f) Transwell assay for cell migration and invasion. (g and h) Western blot for Ki-67, Bcl-2, and MMP9 expression levels in transfected cells. **P < 0.01, ***P < 0.001, or ****P < 0.0001.
in CRC, in agreement with the recent work [10]. Furthermore, we presented a significant clinical association of circ-CSPP1 with the poor prognosis of CRC patients. Ki-67 is a standard marker of proliferation, and MMP9 is closely related to tumor cell metastasis [25,26]. In the current work, loss-of-function phenotypes of circ-CSPP1 uncovered that circ-CSPP1 was a positive regulator of CRC cell malignant behaviors in vitro and tumor growth in vivo. As previously reported for other circRNAs [27,28], circ-CSPP1 was stable and resistant to RNase R due to the lack of 5′ and 3′ ends [4]. Additionally, circ-CSPP1 was mainly present in the cytoplasm of CRC cells, providing the possibility for its interaction with miRNAs in the RNA-induced silencing complex where miRNAs silence gene expression [11].
Previous studies have demonstrated the conflicting roles of miR-431 in cancer development [29][30][31]. These contradictory findings might partially be due to the different tumor types in these studies, where miR-431 served as a tumor suppressor in various cancers, such as pancreatic cancer [29] and breast cancer [30], and facilitated the metastasis of pancreatic neuroendocrine tumor [31].
In the present report, we first identified miR-431 as a directly targeted miRNA of circ-CSPP1. Our data also showed the negative regulation of miR-431 on CRC cell malignant behaviors, consistent with the previous work [14]. Furthermore, our study demonstrated miR-431 as a functionally downstream mediator of circ-CSPP1 in modulating CRC cell malignant behaviors. Hu et al. reported that hsa_circ_0001742 promoted the development of tongue squamous cell carcinoma by miR-431 [32]. Li et al. highlighted that hsa_circ_0005075 functioned as a tumor promoter in hepatocellular carcinoma via reducing miR-431 activity [33].
LASP1, an actin-binding structural protein, is a nuclear transcriptional regulator in various cancers [34,35]. LASP1 was widely reported as a potent tumor driver in CRC via activating two survival and proliferation pathways [36,37]. In the present study, we first identified that LASP1 was a functionally important effector of miR-431 in modulating CRC cell malignant behaviors. Similarly, recent work reported that several other miRNAs, such as miR-625-5p and miR-133a, hindered CRC progression by directly targeting LASP1 [38,39]. Furthermore, our study first illuminated the modulation of circ-CSPP1 on LASP1 expression via miR-431 in CRC cells. Such analyses are hampered at present by the lack of the direct evidence in vivo of the novel mechanism, the circ-CSPP1/miR-431/ LASP1 axis, in regulating CRC progression. Previous work reported the promoting effect of circ-CSPP1 on the stemness of CRC cells through the miR-193a-5p/ COL1A1 axis [10]. Recent studies also identified the oncogenic role of circ-CSPP1 in human carcinogenesis by regulating the miR-361-5p/ITGB1 or miR-577/CCNE2 axis [22,24]. These findings suggest that there may be Figure 6: The enforced expression of miR-431 regulated CRC cell progression in vitro by downregulating LASP1. HCT-116 and SW480 cells were transfected with miR-NC mimic, miR-431 mimic, miR-431 mimic + pcDNA, or miR-431 mimic + LASP1, followed by the determination of LASP1 protein level by western blot (a), cell viability by CCK-8 assay (b), cell colony formation by colony formation assay (c), cell apoptosis by flow cytometry (d), cell migration and invasion by transwell assay (e and f), and Ki-67, Bcl-2, and MMP9 expression levels by western blot (g and h). (i) LASP1 protein level by western blot in HCT-116 and SW480 cells transfected with si-NC, si-circ-CSPP1, si-circ-CSPP1 + anti-miR-NC or si-circ-CSPP1 + anti-miR-431. pcDNA: negative control plasmid, LASP1: LASP1 overexpression plasmid. **P < 0.01, ***P < 0.001, or ****P < 0.0001. many other miRNA/mRNA axes that remain to be identified in the regulation of circ-CSPP1.
Collectively, the findings of the present study demonstrated the oncogenic effect of circ-CSPP1 on CRC partially by the modulation of the miR-431/LASP1 axis. We first identified the circ-CSPP1/miR-431/LASP1 axis as a novel regulatory mechanism in CRC, providing key evidence for circ-CSPP1 as a potential biomarker for CRC management.
Funding information: The authors state no funding involved.

Conflict of interest:
The authors state no conflict of interest.
Data availability statement: The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.