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Licensed Unlicensed Requires Authentication Published by De Gruyter January 8, 2016

Reproductive and cytogenetic characterization in Passiflora sublanceolata

  • Gabriela de O. Belo , Margarete M. Souza EMAIL logo , Viviane de O. Souza and Cláusio Antônio F. de Melo
From the journal Biologia


Reproductive biology (pollen-ovule ratio, pollen viability, germination in vitro pollination and stigma receptivity in vivo) and karyotype characterization by classical and molecular techniques were performed in Passiflora sublanceolata. The pollen-ovule ratio was 83.9, suggesting that this species is facultative autogamous. Pollen viability was below 70% during all anthesis period (6:00 a.m. to 12:00 p.m.). Low in vitro germination rates were observed after anthesis beginning, with none percentage at one, four and six hours and medium percentage (52.9%) at five hour. The stigma remained receptive during the whole anthesis (100%). The average fertilization percentages resulting from controlled pollinations varied between 8.4% at six hour after anthesis beginning and 50% at two and three hour. The fruit characteristics did not show significant differences by the effects of pollination time. The chromosome number was 2n = 22. The average chromosome length and the haploid chromosome length were 1.60 μm and 17.61 μm, respectively. Six CMA+3 /DAPI terminal blocks were observed. By means of the FISH technique it was found that the species presented six 45S rDNA terminal sites, two 5S rDNA sites, terminal and pericentromeric, and telomeric hybridization sites associated only to the terminal regions. Passiflora sublanceolata has great reproductive potential and cytogenetic stability as diploid plant. The results obtained in this work will assist in the use of P. sublanceolata as genitor in interspecific hybridizations with other taxa for breeding programs primarily aimed to obtain hybrids for ornamental plant market.


Abdelgadir H.A., Johnson S.D. & Van Staden J. 2012. Pollen viability, pollen germination and pollen tube growth in the biofuel seed crop Jatropha curcas (Euphorbiaceae). S. Afr. J. Bot. 79: 132-139.Search in Google Scholar

Abdullateef R.A., Zakaria N.H., Hasali N.H. & Osman M. 2012. Studies on pollen viability and germinability in accessions of Stevia rebaudiana Bertoni. Int. J. Biol. 4: 72-79.Search in Google Scholar

Abreu P.P., Souza M.M., Santos E.A., Pires M.V., Pires M.M. & Almeida A.-A.F. 2009. Passion flower hybrids and their use in the ornamental plant market: perspectives for sustainable development with emphasis on Brazil. Euphytica 166: 307-315.Search in Google Scholar

Akamine E.K. & Girolami D.G. 1959. Pollination and fruit set in the yellow passion fruit. Hawaii Agric. Exp. Sta. 39: 1-44.Search in Google Scholar

Allard R.W. 1960. Principios do melhoramento genetico de plantas, Blucher-USAID, São Paulo, 381 pp.Search in Google Scholar

Alexander M.P. 1969. Differential staining of aborted and nonaborted pollen. Stain Tech. 44: 117-122.Search in Google Scholar

Amela Garcia M.T. & Hoc P.S. 1997. Floral biology and reproductive system of Passiflora caerulea (Passifloraceae). Beitr. Biol. Pflanz.70: 1-20.Search in Google Scholar

Amela Garcia M.T. & Hoc P.S. 1998a. Aspectos de la biologia floral y el sistema reproductivo de Passiflora mooreana (Passifloraceae). Darwiniana 35: 9-27.Search in Google Scholar

Amela Garcia M.T. & Hoc P.S. 1998b. Biologia floral de Passiflora foetida (Passifloraceae). Rev. Biol. Trop. 46: 9-20.Search in Google Scholar

Amela Garcia M.T., Galati B.G. & Anton A.M. 2002. Microsporogenesis, microgametogenesis and pollen morphology of Passiflora spp. (Passifloraceae). Bot. J. Linn. Soc. 139: 383-394.Search in Google Scholar

Amela Garcia M.T. 2008. Breeding system and related floral features of Passiflora suberosa (Passifloraceae) under natural and experimental conditions in Argentina. Bol. Soc. Argent. Bot. 43: 83-93.Search in Google Scholar

Amela Garcia M.T. & Gottsberger G. 2009. Tama´no y viabilidad del polen, relacion polen/ovulos y sistema reproductivo en cuatro especies del genero Passiflora. Bol. Soc. Argent. Bot. 44 (Supl.): 167.Search in Google Scholar

Amorim J.S., Souza M.M., Viana A.J.C. & Freitas J.C.O. 2011. Self-, cross- and interspecific pollinations in Passiflora capsularis and P. rubra. Rev. Bras. Frutic. 34: 537-544.Search in Google Scholar

Benevides C.R. 2006. Biologia floral e poliniza,cão de Passifloraceae nativas e cultivadas na região Norte Fluminense-RJ. Unpublished MSc. Dissertation, Universidade Estadual do Norte Fluminense Darcy Ribeiro.Search in Google Scholar

Bernacci L.C., Meletti L.M.M., Soares-Scott M.D. & Passos I.R.S. 2005. Especies de maracuja: caracteriza,cão e conserva ,cão da biodiversidade, pp. 559-586. In: Faleiro F.G. Junqueira N.T.V. & Braga M.F. (eds), Maracuja: germoplasma e melhoramento genetico. Vol 1. Planaltina: Embrapa Cerrados.Search in Google Scholar

Biondo E. & Battistin A. 2001. Compara,cão da efici˛encia de diferentes corantes na estimativa da viabilidade de grãos de polen em especies dos g˛eneros Eriosema (DC.) G. Don e Rhynchosia Lour (Leguminosae-Faboideae), nativas na região Sul do Brasil. Bioikos 15: 39-44.Search in Google Scholar

Bowden W.M. 1945. A list of chromosome numbers in higher plants. II. Menispermaceae to Verbenaceae. Am. J. Bot. 32: 123-201.Search in Google Scholar

Brito A.C., Souza J.D., Rebou,cas T.N.H. & Amaral C.L.F. 2010. Propriedades do polen e do estigma de Ocimum basilicum L. (cultivar Maria Bonita) para aumentar a efici˛encia de cruzamentos em programas de melhoramento. Rev. Bras. de Plantas Med. 12: 208-214.Search in Google Scholar

Brown A.H.D. 1989. Genetic characterization of plant mating systems, pp. 145-162. In: Brown A.H.D., Clegg M.T., Kahler A.L. & Weir B.S. (eds), Plant populations genetics, breeding and genetics resources, Vol 1. Sinauer Publishing, Sunderland.Search in Google Scholar

Bruckner C.H., Casali V.W.D., Moraes C.F., Regazzi A.J & Silva E.A.M. 1995. Self-incompatibility in passion fruit (Passiflora edulis Sims). Acta Hortic. 370: 45-57.Search in Google Scholar

Bruckner C.H. & Otoni W.C. 1999. Hibrida,cão em maracuja, pp. 379-399. In: Borem A. (ed.), Hibrida,cão artificial de plantas. Vol 1. Universidade Federal de Vi,cosa, Vi,cosa.Search in Google Scholar

Bruckner C.H., Silva M.M., Falleiro T.M., Andrade B.B. & Moreira A.E. 2000. Viabilidade do polen de maracujazeiro sob diferentes condi,c˜oes de armazenamento. Rev. Ceres 47: 523-531.Search in Google Scholar

Cervi A.C. 2005. Especies de Passiflora L. (Passifloraceae) publicadas e descritas nos ultimos 55 anos (1950-2005) na America do Sul e principais publica,c˜oes brasileiras. Estud. de Biol. 27: 19-24.Search in Google Scholar

Coelho A.P.D, Morais K.P., Dail Laughinghouse I.V.H., Giacomini S.J. & Tadesco S.B. 2012. Pollen grain viability in accessions of Crotalaria juncea L. (Fabaceae). Agroci˛encia 46: 481-487.Search in Google Scholar

Cruden R.W. 1977. Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution 31: 32-46.Search in Google Scholar

Cruz C.D. 2006. Estatistica experimental e matrizes. Universidade Federal de Vi,cosa, Vi,cosa, 285 pp.Search in Google Scholar

Cruz T.V., Roza F.A., Viana A.J.C., Belo G.O., Fonseca J.W.S. & Souza M.M. 2008. Germina,cão in vitro de grãos de polen em Passiflora suberosa para sua utiliza,cão em hibrida,cão interespecifica. Rev. Bras. Frutic. 30: 875-879.Search in Google Scholar

Dafni A. 1992. Pollination ecology: a practical approach, Oxford University Press, New York.Search in Google Scholar

Dafni A., Pacini E. & Nepi M. 2005. Pollen and stigma biology, pp. 83-146. In: Dafni A. & Kevan H.B.C. (eds), Practical pollination biology, Vol 1. NHBS, Cambridge.Search in Google Scholar

De Jong T. & Klinkhamer P.G.L. 2005. Evolutionary ecology of plant reproductive strategies, Cambridge University Press, Cambridge.Search in Google Scholar

Dumas C. & Knox R.B. 1983. Callose e determination of pistil viability and incompatibility. Theor. Appl. Gen. 67: 1-10.Search in Google Scholar

Fajkus J., Sykorova E. & Leitch A.R. 2005. Techniques in plant telomere biology. BioTechniques 38: 233-243.Search in Google Scholar

Ferreira F.R. 1994. Germoplasma de Passiflora no Brasil, pp. 24-26. In: São Jose A.R. (ed.), Maracuja: produ,cão e mercado. UESB, Vitoria da Conquista/BA.Search in Google Scholar

Frazon R.C., Corr˛ea E.R. & Raseira M.C.B. 2005. In vitro pollen germination of feijoa (Acca sellowiana (Berg) Burret). Crop. Breed. Appl. Biot. 5: 229-233.Search in Google Scholar

Fuchs J., Brandes A. & Schubert I. 1995. Telomere sequence localization and karyotype evolution in higher plants. Plant. Syst. Evol. 196: 227-241.Search in Google Scholar

Galen C. & Plowright R.C .1987. Testing the accuracy of using peroxidase activity to indicate stigma receptivity. Can. J. Bot. 65: 107-111.Search in Google Scholar

Gaude T. & McCormick S. 1999. Signaling in pollen-pistil interactions. Cell Dev. Biol. 10: 139-147.Search in Google Scholar

Guerra M. & Souza M.J. 2002. Como observar cromossomos - um guia de tecnicas em citogenetica vegetal, animal e humana, FUPEC, Ribeirão Preto, 131 pp.Search in Google Scholar

Harvey M.J. 1966. IOPB - Chromosome number reports VII. Taxon 15: 155-163.Search in Google Scholar

Herbario IAC 2013. (accessed 31/03/2013).Search in Google Scholar

Heslop-Harrison J. & Heslop-Harrison Y. 1970. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain. Technol. 45: 115-120.Search in Google Scholar

Heitz E. 1927. Pflanzliche chromosomen-zahlen. Tab. Biol. 4: 1-83.Search in Google Scholar

Hoagland D.R. & Arnon D.I. 1950. The water-culture method for growing plants without soil. Calif. Aes. 347: 1-32.Search in Google Scholar

Iapichino G.F. & Loy J.B. 1987. High temperature stress affects pollen viability in bottle gourd. J. Am. Soc. Hortic. Sci. 112: 372-374.Search in Google Scholar

Janaki Ammal E.K. 1945. Chromosome atlas of cultivated plants, p. 114. In: Darlington C.D. & Janaki Ammal. E.K. (eds), George Allen and Unwin Ltd. Vol 1. London.Search in Google Scholar

Johansen D.A. 1940. Plant microtechnique, McGraw Hill, New York.Search in Google Scholar

Kearns C.A. & Inouye D.W. 1993. Techniques for pollination biologists. Colorado University Press, Niwot, 583 pp.Search in Google Scholar

Lederman I.E. 1987. The involvement of ethylene in fruit development, maturation and ripening of the passion fruit, Passiflora edulis Sims, The Hebrew University of Jerusalem, Jerusalem, 280 pp.Search in Google Scholar

Leitch A.R., Schwarzacher T., Jackson D. & Leitch I.J. 1994. In situ hybridization: a practical guide. BIOS Scientific Publishers Limited, Oxford, 118 pp.Search in Google Scholar

Lersten N.R. 2004. Flowering plant embryology, Blackwell Publishing, Ames.Search in Google Scholar

Loguercio A.P. & Battistin A. 2004. Microsporog˛enese de nove acessos de Syzygium cumini (L.) Myrtaceae e oriundos do Rio Grande do Sul- Brasil. Rev. da FZVA 11: 192-205.Search in Google Scholar

Manju I. & Rawat S.S. 2006. Studies on floral biology of Kagzi lime (Citrus aurantifolia Swingle) under valley conditions of Garhwal Himalaya. Adv. Plant Sci.19: 11-17.Search in Google Scholar

Meletti L.M.M., Bernacci L.C., Soares-Scott M.D. & Passos I.R.S. 2005. Melhoramento genetico do maracuja: passado e futuro, pp. 55-78. In: Faleiro F.G., Junqueira N.T.V. & Braga M.F. (eds), Maracuja: germoplasma e melhoramento genetico. Vol 1. Embrapa Cerrados, Planaltina/DF.Search in Google Scholar

Melo N.F., Cervi A.C. & Guerra M. 2001. Karyology and cytotaxonomy of the genus Passiflora L. (Passifloraceae). Plant Syst. Evol. 226: 69-84.Search in Google Scholar

Melo N.F. & Guerra M. 2003. Variability of the 5S and 45S rDNA sites in Passiflora L. with species with distinct base chromosome numbers. Ann. Bot., London 92: 309-316.Search in Google Scholar

Melo C.A.F., Souza M.M., Abreu P.P. & Viana A.J.C. 2014. Karyomorphology and GC-rich heterochromatin pattern in Passiflora (Passifloraceae) wild species from Decaloba and Passiflora subgenera. Flora. 209: 620-631.Search in Google Scholar

Menzel C.M.,Winks C.W. & Simpson D.R. 1989. Passion fruit in Queensland. 3. Orchard management. Queensl. Agr. J. 115: 155-164. Search in Google Scholar

Pearse A.G.E. 1972. Histochemistry, theoretical and applied, Churchill, London. Ramos J.D., Chalfun N.N.J., Pasqual M. & Rufini J.C.M. 2002. Produ,cão de mudas de plantas frutiferas por semente. Inf. Agropec. 23: 64-72.Search in Google Scholar

Rojas G.G. & Medina V.M. 1996. Vingamento de frutos do maracujazeiro amarelo. Rev. Bras. Frutic.18: 283-288.Search in Google Scholar

Santos E.A., Souza M.M., Abreu P.P., Concei,cão L.D.H.C.S., Araujo I.S., Viana A.P., Almeida A.-A .F. & Freitas J.C.O. 2012. Confirmation and characterization of interspecific hybrids of Passiflora L. Euphytica 184: 389-399.Search in Google Scholar

Silva M.M., Bruckner C.H., Pican,co M. & Cruz C.D. 1999. Fatores que afetam a germina,cão do grão de polen do maracuja amarelo: meios de cultura e tipos de agrotoxicos. Pesq. Agropec. Bras. 34: 347-352.Search in Google Scholar

Singh R.J. 2002. Plant cytogenetics, CRC Press, Florida, 512 pp.Search in Google Scholar

Sousa, J.S.I. & Meletti, L.M.M. 1997. Maracuja: especies, variedades e cultivo, FEALQ, Piracicaba, 179 pp.Search in Google Scholar

Souza M.M., Pereira T.N.S. & Martins E.R. 2002. Microsporog˛enese e microgametog˛enese associadas ao tamanho do botão floral e da antera e viabilidade polinica em maracujazeiroamarelo (Passiflora edulis Sims f. flavicarpa Degener). Ci˛enc. Agrotec. 26: 1209-1217.Search in Google Scholar

Souza M.M., Pereira T.N.S., Viana A.P., Pereira M.G., Bernacci L.C., Sudre C.P. & Silva L.C. 2003. Meiotic irregularities and pollen viability in Passiflora edmundoi Sacco (Passifloracaeae). Caryologia 56: 161-169.Search in Google Scholar

Souza M.M., Pereira T.N.S., Viana A.P., Pereira M.G., Amaral Jr A.T. & Madureira H.C. 2004a. Flower receptivity and fruit characteristics associated to time of pollination in the yellow passion fruit Passiflora edulis Sims f. flavicarpa Degener (Passifloracaeae). Sci. Hortic. Amsterdam 101: 373-385.Search in Google Scholar

Souza M.M., Pereira T.N.S., Viana A.P., Silva L.C. & Sudre C.P. 2004b. Pollen viability and fertility in wild and cultivated Passiflora species (Passifloraceae). Beitr. Biol. Pflanz. 73: 1-18.Search in Google Scholar

Souza M.M., Palomino G., Pereira T.N.S., Pereira M.G. & Viana A.P. 2004c. Flow cytometric analysis of genome length variation in some Passiflora species. Hereditas 141: 31-38.Search in Google Scholar

Souza M.M., Pereira T.N.S. & Vieira M.L.C. 2008. Cytogenetic studies in some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species. Braz. Arch. Biol. Techn. 51: 247-258.Search in Google Scholar

Souza M.M., Urdampilleta J.D. & Forni-Martins E.R. 2010. Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora. Genet. Mol. Res. 9: 2148-2155.Search in Google Scholar

Twell D. 1995. Diphtheria toxin-mediated cell ablation in developing pollen: vegetative cell ablation blocks generative cell migration. Protoplasma 187: 144-154.Search in Google Scholar

Ulmer T. & MacDougal J.M. 2004. Passiflora - Passionflowers of the world, Timber Press, Portland, 430 pp.Search in Google Scholar

Vanderplank J. 2000. Passion flowers, 3rd ed., the MIT Press, Cambridge, 224 pp.Search in Google Scholar

Viana A.P., Pereira T.N.S., Pereira M.G., Souza M.M., Maldonado J.F.M. & Amaral Jr A.T. 2003. Genetic diversity among yellow passion fruit commercial genotypes and among Passifloras species using RAPD. Rev. Bras. Frutic. 25: 489-493.Search in Google Scholar

Viana A.J.C. & Souza M.M. 2012. Comparative cytogenetic between the species Passiflora edulis and Passiflora cacaoensis. Plant Biol.14: 820-827.Search in Google Scholar

Westgate M.E., Lizaso J. & Batchelor W. 2003. Quantitative relationships between pollen shed density and grain yield in maize. Crop. Sci. 43: 934-942.Search in Google Scholar

Yotoko K.S.C., Dornelas M.C., Togni P.D., Fonseca T.C., Salzano F.M., Bonatto S.L. & Freitas L.B. 2011. Does variation in genome sizes reflect adaptive or neutral processes? New clues from Passiflora. PLoS ONE 6(3): e18212. doi:10.1371/journal.pone.0018212Search in Google Scholar

Received: 2014-10-11
Accepted: 2015-5-29
Published Online: 2016-1-8
Published in Print: 2015-6-1

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