Abstract
Aegilops searsii (2n = 2x = 14, SsSs), a wild relative of wheat, is a potentially important source of agriculturally useful genes for wheat breeding. The incorporation of Ae. searsii chromosome segments 1Ss or 2Ss into the wheat genome could significantly improve wheat flour quality and powdery mildew resistance, respectively. However, monitoring of wheat-Ae. searsii introgressions involving 1Ss or 2Ss chromosomes is difficult due to the lack of Ae. searsii-specific molecular markers. In this study, a total of 476 EST- or SSR-based primer pairs were synthesized and screened against a set of Chinese Spring (CS)-Ae. searsii addition lines and the control background variety CS. As a result, 17 molecular markers specific either for 1Ss or 2Ss chromosomes have been developed, among them, 1, 4, 2 and 10 markers have been assigned to chromosome arms 1SsS, 1SsL, 2SsS and 2SsL, respectively. All 17 markers can be successfully used to screen segregating populations. These markers are completely consistent with genomic in situ hybridization (GISH) results, indicating that these markers could be used for detecting Ae. searsii chromatin in wheat background.
*Electronic supplementary material. The online version of this article (DOI: 10.1515/biolog-2016-0003) contains supplementary material, which is available to authorized users.
Acknowledgements
We are thankful to the seed providers. We particularly thank Dr. I. Dundas, School of Agriculture, Food and Wine, The University of Adelaide, Australia, for reviewing and editing of the manuscript. This research was funded by National Natural Science Foundation of China (31201203), China Postdoctoral Science Foundation (2013T60850), Project of Independent Innovation for Key Technology of Shandong Province, Program for youth talent of SAAS (1-18-024), Seed industry foundation grant to Taishan Scholar, Project of crop seed industrialization of Shandong Province, Science & Technology Development Plan of Shandong Province (2013GNC11025) and the modern agricultural industry technology system (CARS-03-1-8).
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