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Licensed Unlicensed Requires Authentication Published by De Gruyter February 25, 2016

Bacterial communities inhabiting toxic industrial wastewater generated during nitrocellulose production

  • Elvira E. Ziganshina , Emil M. Ibragimov , Olga N. Ilinskaya and Ayrat M. Ziganshin EMAIL logo
From the journal Biologia


Investigating the microbial community structure and composition of toxic industrial wastes contaminated with nitrocellulose and various by-products is crucial for understanding the fate of these pollutants in the environment and for the development and application of efiective bioremediation processes. In this study, we investigated the chemical properties and toxic potential of wastewater generated during nitrocellulose production. The analyzed wastewater from settling pond contained nitrocellulose powder particles as well as increased ammonium (570–760 mg/L), sulfate (1625– 2045 mg/L) and sulfite (864–1014 mg/L) concentrations. The toxicity test results demonstrated that the wastewater samples present acute toxicity for Paramecium caudatum and Daphnia magna. Furthermore, bacterial community structure in the samples was characterized by pyrosequencing of 16S rRNA genes. Phylogenetic analysis of bacterial sequences indicated that Proteobacteria, Bacteroidetes and Firmicutes were the main phyla in the sample near inlet, whereas various phylotypes of the phyla Proteobacteria, Chlorobi, Bacteroidetes and Gemmatimonadetes dominated in the sample near outlet. Some bacterial members observed in the current work can be considered as agents capable of performing biodegradation of various hazardous contaminants, indicating that the described bacterial communities have a high potential for the development of efiective bioremediation strategies.

Electronic supplementary material. The online version of this article (DOI: 10.1515/biolog-2016-0014) contains supplementary material, which is available to authorized users.

The paper was presented at the 13th International Symposium on Aquatic Oligochaeta, Brno, Czech Republic, 7–11 September, 2015

Abbreviations: S I, sample near inlet; S O, sample near outlet.


This work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University. The reported study was funded by the Russian Foundation for Basic Research according to the research project No. 14-04-31694. We thank Dr. R.Z. Agzamov for support during the sampling. Pyrosequencing was performed at the Interdisciplinary Center for collective use of Kazan Federal University for cellular, genomic and post-genomic research in Volga Region (Russia).


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Received: 2015-10-27
Accepted: 2016-1-16
Published Online: 2016-2-25
Published in Print: 2016-1-1

© 2016 Institute of Molecular Biology, Slovak Academy of Sciences

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