Abstract
Expansin proteins play a key role in inducing the extension and relaxation of plant cell walls. To understand the physiological role of expansins in lotus crop, Nelumbo nucifera Gaertn, during plant development, we cloned an expansin gene designated as NnEXPA1 using the rapid amplification of cDNA ends technique. The full-length cDNA of NnEXPA1 was 1,456 bp encoding a protein of 250 amino acids. Gene structure of NnEXPA1 showed that it cosists of three exons and two introns. Phylogenetic tree and sequence analysis indicated that NnEXPA1 belongs to theα -expansin subfamily. Results of the real-time semi-quantitative PCR demonstrated that NnEXPA1 were difierentially expressed within all tissues except root during the plant development. Expression of NnEXPA1 in leaf and rhizome were dramatically decreased during the initial to later stages of rhizome-development. In lotus root, however, NnEXPA1 seemed to exhibit a stable expression pattern. Our results indicated that the transcript accumulation of NnEXPA1 between the initial and early stages of rhizome development may be an important factor during rhizome size determination.
The paper was presented at the 13th International Symposium on Aquatic Oligochaeta, Brno, Czech Republic, 7–11 September, 2015
Abbreviations: CBM63, carbohydrate binding module family 63; DPBB, double-psiβ -barrel; EXPA,α -expansin; GH45, glycoside hydrolase family 45; ORF, open reading frame; qPCR, real-time semi-quantitative PCR; RACE, rapid amplification of cDNA ends; UTR, untranslated regions.
Acknowledgements
This work was supported by the National Science and Technology Supporting Program (No. 2012BAD27B01). The authors are very grateful to Mr. Jian-Xiong Wang and Mrs. Xia Yu (Wuhan University) for their help in the cultivation of study materials.
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