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Licensed Unlicensed Requires Authentication Published by De Gruyter April 20, 2016

In vitro evaluation of indole-3-carboxaldehyde on Vibrio parahaemolyticus biofilms

Murugan Rajalaxmi, Vivekanandham Amsa Devi and Shunmugiah Karutha Pandian
From the journal Biologia

Abstract

This study was aimed to explore antibiofilm agents from the hitherto underexplored Palk Bay seawater bacteria. The cell free culture supernatant of the isolate Marinomonas sp. showed profound antibiofilm activity against Vibrio parahaemolyticus ATCC 17802. The active principle responsible for antibiofilm activity was identified as indole-3-carboxaldehyde (ICA) after bioassay guided purification and gas chromatography-mass spectrometry analysis. Further, in vitro antibiofilm activity of ICA was confirmed through light microscopy, confocal imaging, scanning electron microscopy and biofilm disruption studies. In addition, ICA efficiently reduced the swarming motility of the pathogen and promoted the swimming ability. Furthermore, the control of biofilms and swarming efficiency by quorum sensing pathway of the pathogen was modulated by ICA, which was substantiated using real-time analysis for opaR, cpsA, and lafA genes. This study divulged the efficacy of ICA as an antibiofilm agent against V. parahaemolyticus in vitro.

Acknowledgements

The authors thankfully acknowledge the Bioinformatics Infrastructure Facility funded by Department of Biotechnology, Government of India [Grant No. BT/BI/25/012/2012 (BIF)], the instrumentation facility provided by Department of Science and Technology, Government of India through PURSE [Grant No.SR/S9Z-23/2010/42 (G)] & FIST (Grant No.SR-FST/LSI-087/2008), and University Grants Commission, New Delhi, through SAP-DRS1 [Grant No.F.328/2011 (SAP-II)].

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Abbreviations
BIC

biofilm inhibitory concentration

CFCS

cell-free culture supernatant

CLSM

confocal laser scanning microscopy

DPA

2,6-di-O-palmitoyl-L-ascorbic acid

GC-MS

gas chromatography mass spectrometry

ICA

indole-3-carboxaldehyde

LB

Luria Bertani

LM

light microscopy

MIC

minimal inhibitory concentration

mLB

marine Luria Bertani

OD

optical density

QS

quorum sensing

SA

stearic acid

SEM

scanning electron microscopy

ZMB

Zobell marine broth.

Received: 2015-12-19
Accepted: 2016-3-18
Published Online: 2016-4-20
Published in Print: 2016-3-1

© 2016 Institute of Molecular Biology, Slovak Academy of Sciences