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Licensed Unlicensed Requires Authentication Published by De Gruyter April 20, 2016

Inhibitory effect of Murraya koenigii against Candida albicans virulence and biofilm development

  • Subramanian Muthamil and Shunmugiah Karutha Pandian EMAIL logo
From the journal Biologia


In this study, anti-infective potential of the medicinal plant Murraya koenigii was assessed through in vitro assays and microscopic analysis. The methanolic leaf extract of M. koenigii significantly inhibited the major virulence factors of Candida albicans, such as biofilm formation, yeast-to-hyphal transition, cell surface hydrophobicity, hemolysin production and filamentation. Further purification and molecular characterization of the active lead is expected to give a novel anticandidal agent for the treatment of Candida infection.


The authors thankfully acknowledge the Bioinformatics Infrastructure Facility funded by Department of Biotechnology, Government of India [Grant No. BT/BI/25/015/2012 (BIF)], the instrumentation facility provided by Department of Science and Technology, Government of India through PURSE [Grant No. SR/S9Z-23/2010/42 (G)] & FIST (Grant No. SR-FST/LSI-087/2008), and University Grants Commission (UGC), New Delhi, through SAP-DRS1 [Grant No. F.3-28/2011 (SAP-II)]. SM thanks UGC for financial assistance in the form of a Basic Scientific Research Fellowship [Sanction No. F.25-1/2013-14 (BSR)/7-326/2011 (BSR) dt 30.05.2014].


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confocal laser scanning microscopy


minimum biofilm inhibitory concentration


Murraya koenigii methanolic


microtiter plate


phosphate buffered saline


Sabouraud dextrose agar


scanning electron microscopy




yeast extract peptone dextrose

Received: 2015-12-21
Accepted: 2016-3-18
Published Online: 2016-4-20
Published in Print: 2016-3-1

© 2016 Institute of Molecular Biology, Slovak Academy of Sciences

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