Abstract
Media components were optimized using two-step statistical design of experiments for enhancing endoglucanase/ carboxymethyl cellulase (CMCase) production by Trichoderma reesei RUT C30. A Placket-Burman design identified cellulose concentration and pH as the most significant variables, which influenced the CMCase activity. Central composite design was employed to optimize these selected parameters. The optimal activity was obtained at cellulose concentration 19.7 g/L and pH of 7.2. Under the optimized conditions, CMCase activity was 83.63 ± 1.86 IU/mL and filter paper activity was 2.58 ± 0.2 filter paper units per mL. Enzyme productivity was higher compared to previous reports. The enzyme produced from T. reesei was concentrated and was evaluated for deinking of printed paper, which demonstrated the suitability of the enzyme for this application.
Acknowledgements
ASOI gratefully acknowledges the financial assistance provided by Department of Biotechnology (DBT), Government of India, and The World Academy of Sciences for the Advancement of Science in developing countries (TWAS) in the form of fellowship for supporting his PhD program, of which this study forms a part.
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- Abbreviations
- ANOVA
analysis of variance
- CCD
central composite design
- CMC
carboxymethyl cellulose
- CMCase
carboxymethyl cellulase
- CSL
corn steep liquor
- DAHP
diammonium hydrogenphosphate
- DNS
3’5’-dinitrosalicylic acid
- IU
international unit
© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
