Abstract
Serodiagnostic test for the diagnosis of infestation of camels by the camel nasal fly, Cephalopina titillator was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of antibodies in the camel mucus against salivary gland contents (SGc), excretory-secretory products (ESP), digestive tract contents (DTc) antigens from 2nd and 3rd larval instars of C. titillator sampled in summer and winter. ELISA characterization of larval antigens showed that the presence of C. titillator larvae in camel, Camelus dromedarius, resulted in higher antibody titer in mucus samples of infested camels collected in winter than those collected in summer in both L2 and L3. In L2, the most antigenic secretion was SGc > ESP > DTc, but in L3 ESP > SGc > DTc. The antibody titers in camel mucus are affected by the intensity of infestation with C. titillator.
Acknowledgements
Sincere gratitude goes to Prof. Dr. Ramón Cepeda-Palacios, (Laboratorio de Sanidad Animal, Universidad Autónoma de Baja California Sur, Mexico) for critical reviewing and kind efforts to improve the manuscript. Sincere thanks to Eman Alaaeldin Abdelfattah (Entomology Department, Faculty of Science, Cairo University) for kind help in improving the Figures.
References
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