Abstract
In the present study, The hexaploid species Roegneria kamoji (StStYYHH genomes) in Triticeae produced a single high molecular weight glutenin subunit (HMW-GS) 1St1.2 in its endosperm. 1St1.2 encoded 404 amino acid, which is shorter than previous reported HMW-GSs in Triticeae. The N-terminal domains of 1St1.2 resemble that of the y-type subunits, while their C-terminal domains are similar to the x-type subunits. We discovered many variation types of polypeptides in central repetitive domain. There also have amino acid mutations in the N-terminal domain. The results of phylogenetic analysis suggested that 1St1.2 might be expression from the St genome. Therefore, the results indicated that 1St1.2 is a novel HMW-GS variant or isoform, and the characterization of the subunit can enhance our understanding on the structural differentiation and evolutionary relationships of HMW-GSs in wheat and its wild relatives.
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Nos. 31470305, 31270243), Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003021), and the Science and Technology Bureau and Education Bureau of Sichuan Province, China. The authors are highly thankful to Dr. Zehong Yan of Triticeae Research Institute, Sichuan Agricultural University for his kind helps on experiment discussions.
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