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Licensed Unlicensed Requires Authentication Published by De Gruyter August 11, 2016

Antioxidant status of rabbits after treatment with epicatechin and patulin

Katarina Zbynovska, Peter Petruska, Anna Kalafova, Lubomir Ondruska, Rastislav Jurcik, Lubica Chrastinova, Eva Tusimova, Anton Kovacik and Marcela Capcarova
From the journal Biologia


The aim of this study was to assess antioxidant status by measuring of antioxidant parameters (SOD – superoxide dismutase, GPx – glutatione peroxidase, CAT – catalase, Trolox, TAC – total antioxidant capacity, bilirubin, albumin) and parameters associated with oxidative stress (MDA – malondialdehyde, MDA Adduct – malondialdehyde adduct, H2O2 – hydrogen peroxide) in rabbit blood after five weeks exposure to epicatechin alone or in combination with patulin in vivo. We investigated the protective role of epicatechin against possible negative effect of patulin in rabbits. Adult female rabbits (n = 32) were used in the experiment. Animals were divided into eight groups with four rabbits in each group. In the experiment there were two control groups: one without addition of patulin and epicatechin and one with addition of patulin (10 μg kg−1). Rabbits in the experimental groups received three times a week intramuscular injection of low dose (10 μg kg−1), medium dose (100 μg kg−1) or high dose (1000 μg kg−1) of epicatechin alone or in combination with low dose (10 μg kg−1) of patulin. Patulin alone in low dose significantly (P < 0.05) affected total antioxidant status in comparison with the experimental groups with the highest dose of epicatechin alone (1000 μg kg−1) and in combination with patulin. SOD was negatively affected by patulin. Enzymatic activity of this enzyme was significantly lower (P < 0.05) in the experimental group with patulin in comparison with the experimental group receiving the lowest concentration of epicatechin (10 μg kg−1). Patulin can induce oxidative stress in rabbits in low dose and catechin could have possible protective effect. Our results showed that patulin negatively affected TAC, and SOD in rabbit blood. Further research needs to be focused on the generation of data dealing with antioxidant effects.


This work was financially supported by VEGA scientific grant 1/0760/15. This work was co-funded by European Community under project no 26220220180: Building Research Centre “AgroBioTech”.


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Received: 2016-3-1
Accepted: 2016-6-17
Published Online: 2016-8-11
Published in Print: 2016-7-1

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