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Licensed Unlicensed Requires Authentication Published by De Gruyter August 11, 2016

Isolation and characterization of Vibrio alginolyticus from cultured amphioxus Branchiostoma belcheri tsingtauense

Yuxia Zou, Cuiping Ma, Yanfei Zhang, Zongjun Du, Feng You, Xungang Tan and Pei-Jun Zhang
From the journal Biologia

Abstract

Amphioxus is an intermediary species bridging invertebrates and vertebrates used to study the development and evolution of species and genes. In this study, the dominant bacterial strain was isolated from tail lesions of cultured amphioxus and designated WW1. Challenge by intramuscular microinjection revealed the 50% lethal dose of WW1 to be 9 × 102 bacteria/g amphioxus body weight. Based on 16S rRNA gene sequence, physiological and biochemical characteristics, and species-specific collagenase gene marker, WW1 was identified as Vibrio alginolyticus. To our knowledge, this is the first report on the isolation of V. alginolyticus as pathogen from amphioxus. This study extends the known host range of V. alginolyticus and may provide information helpful in preventing and managing its occurrence in cultured animals.

Acknowledgements

This work was supported by The National Sciences Foundation of China (No. 30972270), the National High-Technology Research and Development Program (863 Program) of China (2008AA092600), and the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02).

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Abbreviations
NS

normal saline

TCBS

thiosulphate citrate bile salts sucrose

Received: 2016-12-12
Accepted: 2016-6-15
Published Online: 2016-8-11
Published in Print: 2016-7-1

©2016 Institute of Molecular Biology, Slovak Academy of Sciences