Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter April 21, 2017

Modulation in arsenic-induced lipid catabolism in Glycine max using proline, 24-epibrassinolide and diphenylene iodonium

  • Vibhuti Chandrakar , Suruchi Parkhey , Amit Dubey and Sahu Keshavkant EMAIL logo
From the journal Biologia

Abstract

Proline, 24-epibrassinolide and diphenylene iodonium are few of the novel antioxidant molecules, involved in growth regulation and abiotic stress tolerance of plants. However, these are scarcely explored in relation to their role in arsenic stress tolerance. Therefore, present study was designed to investigate the involvement of proline, 24-epibrassinolide and diphenylene iodonium in conferring tolerance to Glycine max L. against arsenic toxicity. The results showed that arsenic caused decrease in growth attributes like germination percentage, radicle length and dry mass, which were accompanied by the accumulation of arsenic. The application of arsenic steeply reduced total lipid content while increased the levels of oxidative stress markers such as superoxide anion, hydroxyl radical, hydrogen peroxide, free fatty acid, conjugated diene, lipid hydroperoxide, malondialdehyde and 4-hydroxy-2-nonenal, and the activities of lipase and lipoxygenase. Impressively, proline, 24-epibrassinolide and diphenylene iodonium played their roles as protective agents, and caused enhanced growth and reduced arsenic accumulation. These protective molecules enhanced the total lipid content while reduced the levels of oxidative stress markers and activities of lipase and lipoxygenase. The results indicated that proline, 24-epibrassinolide and diphenylene iodonium served as potential inhibitors of As-induced oxidative stress in Glycine max L.

Acknowledgements

The authors are grateful to the English language corrector, and learned editors and reviewers involved all through the processing of this manuscript. The authors are also like to acknowledge financial assistance awarded to S. Parkhey and V. Chandrakar by University Grants Commission, New Delhi (F.4-1/2006(BSR)/7-145/2007(BSR), dated 24.06.2011) and Department of Science and Technology, New Delhi (INSPIRE fellowship, Dy. No. JS and FA/1884, dated 31.12.2013), respectively. Authors are also grateful to Department of Science & Technology, New Delhi, for financial support in the forms of DST-FIST scheme (Sanction No. 2384/IFD/2014-15, dated 31.07.2014) and Natural Centre for Natural Resources [IR/SO/LU/0008/2011 (SERB)] to the School of Studies in Biotechnology and Pt. Ravishankar Shukla University, Raipur, respectively.

References

Agami R.A. 2014. Applications of ascorbic acid or proline increase resistance to salt stress in barley seedlings. Biol. Plant. 58: 341–347.10.1007/s10535-014-0392-ySearch in Google Scholar

Bustingorri C. & Lavado R.S. 2014. Soybean as affected by high concentrations of arsenic and fluoride in irrigation water in controlled conditions. Agric. Water Manag. 144: 134–139.10.1016/j.agwat.2014.06.004Search in Google Scholar

Chandra J., Tandon M. & Keshavkant S. 2015. Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L. Physiol. Mol. Biol. Plants 21: 215–223.10.1007/s12298-015-0294-2Search in Google Scholar

Chandra J. & Keshavkant S. 2016. Physiological and biochemical changes during seed development and maturation in Madhuca latifolia Roxb. Bangladesh J. Bot. 45: 335–343.Search in Google Scholar

Chandrakar V., Dubey A. & Keshavkant S. 2016a. Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L. J. Soil Sci. Plant Nut. 16: 662–676.10.4067/S0718-95162016005000048Search in Google Scholar

Chandrakar V., Naithani S.C. & Keshavkant S. 2016b. Arsenic–induced metabolic disturbances and their mitigation mechanisms in crop plants: A review. Biologia 71: 367–377.10.1515/biolog-2016-0052Search in Google Scholar

Chandrakar V., Yadu B., Meena R.K., Dubey. A. & Keshavkant S. 2017. Arsenicinduced genotoxic responses and their amelioration by diphenylene iodonium, 24epibrassinolide and proline in Glycine max L. Plant Physiol. Biochem. 112: 74 86.10.1016/j.plaphy.2016.12.023Search in Google Scholar

Forino L.M.C., Castiglione M.R., Bartoli G., Balestri M., Andreucci A. & Tagliasacchi A.M. 2012. Arsenic-induced morphogenic response in roots of arsenic hyperaccumulator fern Pteris vittata. J. Hazard Mat. 235–236: 271–278.10.1016/j.jhazmat.2012.07.051Search in Google Scholar

Gallego S.M., Benavides M.P. & Tomaro M.L. 1996. Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci. 121: 151–159.10.1016/S0168-9452(96)04528-1Search in Google Scholar

Gidrol X., Serghini A., Noubhani B., Mocquot B. & Mazliak P. 1989. Biochemical changes induced by accelerated aging in sunflower seeds: lipid peroxidation and membrane damage. Physiol. Plant. 76: 591–597.10.1111/j.1399-3054.1989.tb05484.xSearch in Google Scholar

Halliwell B., Gutteridge J.M.C. & Auroma O. 1987. The deoxyribose method: a simple ‘test tube’ assay for determination of rate constants for reactions of hydroxyl radicals. Ann. Biochem. 165: 215–219.10.1016/0003-2697(87)90222-3Search in Google Scholar

Hayat S., Maheshwari P., Wani A.S., Irfan M., Alyemeni M.N. & Ahmad A. 2012. Comparative effect of 28 homobrassinolide and salicylic acid in the amelioration of NaCl stress in Brassica juncea L. Plant Physiol. Biochem. 53: 61–68.10.1016/j.plaphy.2012.01.011Search in Google Scholar

Hodges D.M., Delong J.M., Forney C.F. & Prang R.K. 1999. Improving the thiobarbituric acid reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207: 604–611.10.1007/s004250050524Search in Google Scholar

Itaya K. & Ui M. 1965. Colorimetric determination of free fatty acids in biological fluids. J. Lipid Res. 6: 16–20.10.1016/S0022-2275(20)39633-4Search in Google Scholar

Jiang M. & Zhang J. 2002. Water stressinduced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J. Exp. Bot. 53: 2401–2410.10.1093/jxb/erf090Search in Google Scholar PubMed

Kaur S., Singh H.P., Batish D.R., Negi A., Mahajan P., Rana S. & Kohli R.K. 2012. As inhibits radicle emergence and elongation in Phaseolus aureus by altering starchmetabolizing enzymes vis-à-vis disruption of oxidative metabolism. Biol. Trace Elem. Res. 146: 360–368.10.1007/s12011-011-9258-8Search in Google Scholar PubMed

Kazemi N., Khavari-Nejad R.A., Fahimi H., Saadatmand S. & Nejad-Sattari T. 2010. Effects of exogenous salicylic acid and nitric oxide on lipid peroxidation and antioxidant enzyme activities in leaves of Brassica napus L. under nickel stress. Sci. Hortic. 126: 402–407.10.1016/j.scienta.2010.07.037Search in Google Scholar

Keshavkant S. & Naithani S.C. 2010. Chilling induced superoxide production, lipid peroxidation and leakage loss in Shorea robusta seedlings. Indian J. Plant Physiol. 15: 191–196.Search in Google Scholar

Khan M.M., Hendry G.A.F., Atherton N.M. & Vertucci-Walters C.W. 1996. Free radical accumulation and lipid peroxidation in testa of rapidly aged soybean seeds: a light-promoted process. Seed Sci. Res. 6: 101–107.10.1017/S0960258500003123Search in Google Scholar

Lozano-Rodríguez E., Liguera M., Lucena J.J. & Carpena RO. 1995. Evaluation of two different acid digestion methods in closed systems for trace element determination in plants. Quim. Anal. 14: 27–30.Search in Google Scholar

Noctor G., Lelarge-Trouverie C. & Mhamdi A. 2014. The metabolomics of oxidative stress. Phytochemistry 112: 33 53.10.1016/j.phytochem.2014.09.002Search in Google Scholar PubMed

Parkhey S., Naithani S.C. & Keshavkant S. 2012. ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging. Plant Physiol. Biochem. 57: 261–267.10.1016/j.plaphy.2012.06.008Search in Google Scholar PubMed

Parkhey S., Tandan M. & Keshavkant S. 2014. Salicylic acid and acquisition of desiccation tolerance in Pisum sativum seeds. Biotechnology 13: 217–225.10.3923/biotech.2014.217.225Search in Google Scholar

Perfus-Barbeoch L., Leonhardt N., Vavaddeur A. & Forestier C. 2002. Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant J. 32: 539–548.10.1046/j.1365-313X.2002.01442.xSearch in Google Scholar

Raheja R.S., Kaur C., Singh A. & Bhatia I.S. 1973. New colorimetric method for quantitative estimation of phospholipid without acid digestion. J. Lipid Res. 14: 695–702.10.1016/S0022-2275(20)36853-XSearch in Google Scholar

Rai A., Bhardwaj A., Mishra P., Bag S.K., Adhikari B., Tripathi R.D., Trivedi P.K. & Chakrabarty D. 2015. Comparative transcriptional profiling of contrasting rice genotypes shows expression differences during arsenic stress. Plant Gen. 8: 1–14.10.3835/plantgenome2014.09.0054Search in Google Scholar

Ramakrishna B. & Rao S.S.R. 2013. 24Epibrassinolide maintains elevated redox state of AsA and GSH in radish (Raphanus sativus L.) seedlings under zinc stress. Acta Physiol. Plant. 35: 1291–1302.10.1007/s11738-012-1168-7Search in Google Scholar

Ray S., Roy K. & Sengupta C. 2007. Evaluation of protective effect of water extract of Spirulina plantensis (blue green algae) on cisplatin-induced lipid peroxidation. Ind. J. Pharma Sci. 3: 378–382.10.4103/0250-474X.34546Search in Google Scholar

Sangeetha P., Das V.N., Koratker R. & Suryaprabha P. 1990. Increase in free radical generation and lipid peroxidation following chemotherapy in patients with cancer. Free Rad. Biol. Med. 8: 15–19.10.1016/0891-5849(90)90139-ASearch in Google Scholar

Shu H., Ni W., Guo S., Gong Y., Shen X., Zhang X., Xu P. & Guo Q. 2015. Rootapplied brassinolide can alleviate the NaCl injuries on cotton. Acta Physiol Plant. 37: 75–86.10.1007/s11738-015-1823-xSearch in Google Scholar

Siddiqui F., Tandon P.K. & Srivastava S. 2015. Analysis of arsenic induced physiological and biochemical responses in a medicinal plant, Withania somnifera. Physiol. Mol. Biol. Plants 21: 61–69.10.1007/s12298-014-0278-7Search in Google Scholar PubMed PubMed Central

Singh H.P., Batish D.R., Kohli R.K. & Arora K. 2007. As induced root growth inhibition in mung bean is due to oxidative stress resulting from enhanced lipid peroxidation. Plant Growth Regul. 53: 65–73.10.1007/s10725-007-9205-zSearch in Google Scholar

Singh M., Singh V.P., Dubey G. & Prasad S.M. 2015. Exogenous proline application ameliorates toxic effects of arsenate in Solanum melongena L. seedlings. Ecotoxicol. Environ. Saf. 117: 164–173.10.1016/j.ecoenv.2015.03.021Search in Google Scholar PubMed

Singh N. & Bhardwaj R.D. 2016. Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants. Biologia 71: 402–413.10.1515/biolog-2016-0050Search in Google Scholar

Sofo A., Dichio B., Xiloyannis C. & Masia A. 2004. Lipoxygenase activity & proline accumulation in leaves and roots of olive trees in response to drought stress. Physiol. Plant. 121: 58 65.10.1111/j.0031-9317.2004.00294.xSearch in Google Scholar

Sun J., Wang R., Zhang X., Yu Y., Zhao R., Li Z. & Chen S. 2013. Hydrogen sulfide alleviates cadmium toxicity through regulations of cadmium transport across the plasma and vacuolar membranes in Populus euphratica cells. Plant Physiol. Biochem. 65: 67–74.10.1016/j.plaphy.2013.01.003Search in Google Scholar

Tripathi R.D., Singh R., Tripathi P., Dwivedi S., Chauhan R., Adhikari B. & Trivedi P.K. 2014. Arsenic accumulation and tolerance in rootless macrophyte Najas indica are mediated through antioxidants, amino acids and phytochelatin. Aquatic Toxicol. 157: 70–80.10.1016/j.aquatox.2014.09.011Search in Google Scholar

Velikova V., Yordanov I. & Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain treated bean plants : protective role of exogenous polyamines. Plant Sci. 151: 59–66.10.1016/S0168-9452(99)00197-1Search in Google Scholar

Yadu B., Chandrakar V. & Keshavkant S. 2016. Responses of plants towards fluoride: An overview of oxidative stress and defense mechanisms. Fluoride 49: 293–302.Search in Google Scholar

Ye N., Zhu G., Liu Y., Zhang A., Li Y., Liu R., Shi L., Jia L. & Zhang J. 2012. Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds. J. Exp. Bot. 63: 1809–1822.10.1093/jxb/err336Search in Google Scholar PubMed PubMed Central

Received: 2016-7-7
Accepted: 2017-1-16
Published Online: 2017-4-21
Published in Print: 2017-3-1

© 2017 Institute of Botany, Slovak Academy of Sciences

Downloaded on 22.2.2024 from https://www.degruyter.com/document/doi/10.1515/biolog-2017-0033/html
Scroll to top button