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Licensed Unlicensed Requires Authentication Published by De Gruyter April 28, 2017

The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits

  • Marek Halenar EMAIL logo , Lubica Chrastinova , Lubomír Ondruska , Rastislav Jurcik , Katarina Zbynovska , Eva Tusimova , Anton Kovacik and Adriana Kolesarova
From the journal Biologia


The present in vivo study was designed to reveal whether amygdalin is able to cause changes in the endocrine profile and thus alter the key reproductive and physiological functions, using rabbits as a biological model. 40 adult female rabbits were randomly divided into five groups: the control group without any amygdalin administration, two experimental groups received a daily intramuscular injection of amygdalin at dose 0.6 and 3.0 mg kg-1 b.w. and other two groups were fed by crushed apricot seeds at dose 60 and 300 mg kg-1 b.w., mixed with commercial feed over the period of 28 days. The body weight of each experimental animal was recorded weekly during the whole study. Plasma levels of steroid (progesterone, 17β-estradiol, testosterone), thyroid (triiodothyronine, thyroxine, thyroid-stimulating hormone), as well as anterior pituitary (prolactin, luteinizing hormone) hormones were assessed by ELISA. Intramuscular application of amygdalin did not affect (P ≥ 0.05) the plasma levels of none of the endocrine regulators selected. Oral form of amygdalin did not induce significant changes in the plasma levels of examined hormones either. Similarly, no clinically noticeable changes in the average body weight of experimental animals were observed. Our findings indicate that intramuscular and oral application of amygdalin did not significantly affect the plasma levels of studied endocrine regulators in experimental rabbits. In this in vivo study, no obvious beneficial or negative effects of amygdalin on the physiological functions of female rabbits were demonstrated. Short-term intake of amygdalin at the recommended doses does not represent risk for animal health.


This work was financially supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic projects no. 1/0039/16, 011SPU-4/2016 KEGA, APVV-0304-12, and European Community under project no. 26220220180: Building Research Centre “AgroBioTech”.

  1. Declaration of interest: The authors have reported that no competing interests exist.


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Received: 2016-11-3
Accepted: 2017-2-17
Published Online: 2017-4-28
Published in Print: 2017-4-25

© 2017 Institute of Zoology, Slovak Academy of Sciences

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