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Licensed Unlicensed Requires Authentication Published by De Gruyter February 21, 2015

Adhesion of human mesenchymal stem cells can be controlled by electron beam-microstructured titanium alloy surfaces during osteogenic differentiation

Sabine Neuss, Claudia Panfil, Daniela Filipa Duarte Campos, Michael Weber, Christian Otten, Uwe Reisgen and Horst Fischer

Abstract

Several studies focusing on bone tissue engineering demonstrated that given microstructuring of an implant surface has a strong effect on its interaction with cells, and their adhesion and differentiation. In the present study, geometrically structured titanium alloy surfaces are shown to be able to guide cell adhesion during differentiation in vitro. For this reason, using an electron beam texturing technique, TiAl6V4 surfaces were selectively targeted in the micrometer range. The effect of such textured titanium alloy surfaces on cell adhesion during osteogenic differentiation was analyzed for human mesenchymal stem cells (MSC), the natural precursor cells of bone tissue. Cytotoxicity, cell viability and differentiation were analyzed. Immunofluorescence stainings demonstrated that in contrast to MSC in an expansion medium, MSC in an osteogenic induction medium produce adhesion proteins such as ß3-integrins and thereby connect in an oriented way to the generated microstructures on titanium alloy surfaces. These results are of relevance for developing tailored titanium alloy implant surfaces which exhibit an improved cell response.


Corresponding author: Univ.-Prof. Dr.-Ing. Horst Fischer, Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany, Phone: +49 241 8080935, Fax: +49 241 8082027, E-mail:

Acknowledgments

We thank the Department of Orthopaedic Surgery, University Clinic Aachen for providing bone spongiosa and Stephanie Brosig and Norina Labude, Institute of Pathology, RWTH Aachen University for isolation and expansion of human mesenchymal stem cells. We also thank Dr. Lüscher-Firzlaff, Institute of Biochemistry and Molecular Biology, RWTH Aachen University for the supply of PCR equipment and Manfred Bovi, Facility of Electron Microscopy, RWTH Aachen University Hospital for his help in the SEM analysis.

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Received: 2014-9-11
Accepted: 2015-1-20
Published Online: 2015-2-21
Published in Print: 2015-6-1

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