A noninvasive investigation to ascertain the hematocrit (HCT) or packed cell volume (PCV) was conducted on 44 hospitalized dengue hemorrhagic fever (DHF) subjects, male and female aged between 3 and 14 years using bioelectrical impedance analysis (BIA). Among the 44 subjects, 30 subjects were confirmed to be non-structural protein-1 (NS1) positive at the time of admission, whose blood investigations such as HCT level, platelet (PLT) count, aspartate aminotransferase (AST) level and alanine aminotransferase (ALT) level were taken for the classification of risk as low risk (LR) and high risk (HR) DHF. Electrical conductivity of blood reflects a linear correlation with HCT. To provide a better and more accurate estimate of HCT, a dual frequency method is proposed to calculate the conductivities of plasma and blood cells. The resistance at 100 kHz is used to estimate the conductivity of blood cells and the impedance at 5 kHz to estimate the conductivity of plasma. Statistical analysis reveals that the HCT estimated using the proposed dual frequency method shows a significant difference with a single frequency (50 kHz) estimate of HCT and also shows a good correlation with the blood investigation results. In addition, statistical analysis of the proposed method on different fever subjects indicates a significant difference with DHF.
We would also like to thank Dr. P. Narayanan, Dr. R. Sandanalakshmi and Dr. R. Soundravally, co-investigators of the DST project for their immense contributions and help. We also express our thanks to Director and staff of JIPMER Hospital for their cooperation and support.
Research funding: The work was financial supported by the Department of Science and Technology (DST), New Delhi, India (IDP/MED/2012/15 dated 25.3.2014).
Conflict of interest: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all the control and dengue subjects before the study was performed.
Ethical approval: The research related to human use complied with all the relevant national regulations and institutional policies and was performed in accordance to the tenets of the Declaration of Helsinki and has been approval by JIPMER, Puducherry, India (ECR/324/Inst/PY/2013 dated 22.10.2013).
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