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Licensed Unlicensed Requires Authentication Published by De Gruyter September 15, 2010

Cardiac troponin T detection using polymers coated quartz crystal microbalance as a cost-effective immunosensor

Krongkamol Wong-ek, Orawon Chailapakul, Noppadon Nuntawong, Kata Jaruwongrungsee and Adisorn Tuantranont
From the journal

Abstract

Cardiac troponin T (cTnT) detection has been the focus of increased interest due to its role in myocardial infarction diagnosis. In this study, we report a relatively low coat technique to detect cTnT using a quartz crystal microbalance (QCM) sensor. A sensitive detection is achieved by introducing a QCM surface with a carboxylic polyvinyl chloride immobilization layer. The surface morphologies of this polymer film under varied deposition thickness have been investigated by field emission scanning electron microscopy and atomic force microscopy. A cTnT detection result from a modified QCM surface can be obtained within a short response time by a direct detection of the immunoreaction and a direct conversion of mass accumulation into a frequency shift, representing a measurable electrical signal. The relationship between the cTnT concentration and the response current from a QCM sensor shows detectability at the concentration of cTnT as low as 5 ng/ml.


Corresponding author: Krongkamol Wong-ek, Nanoscience and Technology Program, Chulalongkorn University, Bangkok 10330, Thailand

Received: 2010-2-18
Accepted: 2010-6-21
Published Online: 2010-09-15
Published Online: 2010-09-15
Published in Print: 2010-10-01

©2010 by Walter de Gruyter Berlin New York