Accessible Requires Authentication Published by De Gruyter May 31, 2013

Sequences of Mn-sod gene from Pyropia haitanensis (Bangiales, Rhodophyta) and its expression under heat shock

Rui Yang, Wei Liu, Xiao-Long Zhang, Mei-Li Shen, Xue Sun and Hai-Min Chen
From the journal Botanica Marina

Abstract

Manganese superoxide dismutase (Mn-SOD) is an essential enzyme involved in the detoxification of reactive oxygen by dismutation of the superoxide radical anion. In this study, full-length complementary DNA (cDNA) and genomic DNA of Mn-sod genes were cloned from Pyropia haitanensis (phsod), and its expression was monitored using quantitative polymerase chain reaction (qPCR). Scavenging of reactive oxygen species under heat shock was analyzed as well. The full-length cDNA of the phsod gene consists of 935 bp (GenBank accession number: EU715988) with an open reading frame of 675 bp, which encodes 224 amino acids. The length of the genomic sequence is 1416 bp and includes four exons and three introns. A highly conserved Mn-SOD signature (DVWEHAYY) was found. The transit peptide in the N-terminal helps protein targeting to the mitochondria. Results showed that the expression levels of phsod increased 3.4 times compared to controls and then suddenly decreased under a heat shock of 35°C for 30 min. After heat shock exposure and return to normal cultivation temperature, the expression levels of phsod decreased within 1 h of release from the heat shock and then increased within 3 h. SOD activity correlated with the expression of the phsod gene and, as expected, the concentration of ·O2- showed negative correlations with SOD activity.


Corresponding author: Rui Yang, Ministry of Education, Key Laboratory of Applied Marine Biotechnology, Ningbo University, No. 818 Fenghua Road, Ningbo, Zhejiang, 315211, China; and School of Marine Sciences, Ningbo University, No. 818 Fenghua Road, Ningbo, Zhejiang, 315211, China

This research was supported by the National Natural Science Foundation of China No. 40776077, Natural Science Foundation of Zhejiang No. LY12D06003, National Scientific and Technological Support Plan Subject, 2011BAD13B08, Zhejiang Scientific Innovative Team No. 2012R10025-07 and No.2011R09012-06, Ningbo Marine Algae Biotechnology Team No. 2011B81007 and No. 2012A610140 and the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences. We thank Prof. Matthew J. Dring, Prof. Kirsten Heimann and Dr. Gunda Stöber for their assistance in editing this paper.

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Received: 2012-7-1
Accepted: 2013-5-10
Published Online: 2013-05-31
Published in Print: 2013-06-01

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