Accessible Requires Authentication Published by De Gruyter July 19, 2013

Cell division in Azadinium spinosum (Dinophyceae)

Urban Tillmann and Malte Elbrächter
From the journal Botanica Marina

Abstract

Azadinium spinosum is a planktonic dinoflagellate, and a source of azaspiracids, a group of lipophilic toxins of human health concern. Little is known about the biology of this genus, and its phylogenetic position within the Dinophyceae is not yet fully resolved. Here, we present detailed observations regarding its vegetative cell division. As a first sign of cell division, the round interphase nucleus enlarges and becomes elongate in a slightly oblique orientation. The nucleus then divides along its longitudinal axis. Cytokinesis of A. spinosum occurs in motile cells and is of the desmoschisis type, i.e., the parental theca is shared between the two sister cells. Divided cells completely separate before the thecal plates are fully replaced. The anterosinistral daughter cell keeps the parent cell’s apex, including the apical pore complex and all the apical and epithecal intercalary plates, whereas the posterodextral daughter cell keeps, among others, both antapical plates. In general, the course of the fission line follows the plate overlap pattern and is, with a few exceptions, similar to other species of the Gonyaulacales. A literature survey shows that both desmoschisis and eleuteroschisis have been described for Gonyaulacales and Peridiniales, which suggests that mode of division may not be a good ordinal criterion for classification.


Corresponding author: Urban Tillmann, Alfred Wegener Institute, Am Handelshafen12, 27570 Bremerhaven, Germany

Thanks to Fridel Hinz for help with the electron microscope and Consuelo Carbonell-Moore for comments and help with the English. The comments of Matthew Dring and two anonymous reviewers improved the paper and are greatly acknowledged.

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Received: 2013-3-6
Accepted: 2013-6-12
Published Online: 2013-07-19
Published in Print: 2013-08-01

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