This paper documents five new saprobic marine fungi occurring on various substrata in the marine environment. Bacusphaeria nypae gen. et sp. nov. was discovered on bases of fronds of Nypa fruticans in Malaysia and phylogenetically, it belongs to the Tirisporellaceae (Tirisporellales, Diaporthomycetidae, Sordariomycetes) based on 18S and 28S rDNA. Cryptovalsa avicenniae, Ceriosporopsis minuta and Jattaea mucronata also belong to the Sordariomycetes; Cr. avicenniae was saprobic on Avicennia marina collected in Saudi Arabia, J. mucronata on intertidal decaying bark of Rhizophora mucronata while Ce. minuta was found on driftwood collected on a sandy beach in Japan. Phylogeny based on 18S and 28S rDNA placed Cr. avicenniae with other Cryptovalsa species in the Diatrypaceae. Jattaea mucronata groups well in the Calosphaeriales and is closely related to J. algeriensis based on a phylogeny using ITS and 28S rRNA and RPB2 genes. Aegeanispora elanii gen. et sp. nov. is an asexual fungus which produces pycnidia on decaying driftwood collected in the Aegean Sea. Combined 18S and 28S rDNA phylogenetic analyses suggest that it is a member of the Pleosporales, Dothideomycetes. Ceriosporopsis minuta resembles C. capillacea with its tubular, long polar appendages of ascospores but dimensions of ascomata and ascospores for the former species are considerably smaller. Ceriosporopsis is considered the best genus to accommodate C. minuta without support from sequence data. Sammeyersia is established as a new genus to accommodate Lulworthia grandispora, a species unrelated to Lulworthia fucicola, the type species. The diagnostic characteristic for the genus is a melanized region at the base of the neck of the ascomata.
About the authors
Mohamed A. Abdel-Wahab obtained his MSc degree in Marine Fungi from the Sohag University, Egypt. He joined City University of Hong Kong in 1997–2001 and obtained his PhD degree in Marine Mycology in 2001. During 2004–2008, he worked as a Visiting Scholar at the Institute for Biotechnology and Drug-Research, University of Kaiserslautern, Germany, Center of Marine Biotechnology and Biomedicine – Scripps Institution of Oceanography, University of California-San Diego, USA and XBR, Japan Agency for Marine-Earth Science and Technology, Japan where he worked on ecology, molecular phylogeny and natural products of marine fungi. During 2011–2013 he joined the Department of Botany and Microbiology, King Saud University, Saudi Arabia.
Monika C. Dayarathne is a PhD student on taxonomy, phylogeny and evolution of marine fungi under the supervision of Prof. Dr. Kevin D. Hyde at Mae Fah Luang University Thailand and the Kunming Institute of Botany, Chinese Academy of Sciences.
Satinee Suetrong is a Researcher at the Fungal Biodiversity Laboratory, Biodiversity and Biotechnological Resource Research Unit (BBR), National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand. She earned her PhD degree in Microbiology at the Prince of Songkla University, Thailand. Her research interests include marine Dothideomycetous fungi, rock inhabiting and deteriorating fungi in Thailand – isolation, characterization and their natural products and polyunsaturated fatty acid production.
Sheng-Yu Guo obtained her MSc degree from the National Taiwan Ocean University studying phylogeny of marine fungi under the supervision of Ka-Lai Pang. She works as a Research Assistant in Ka-Lai Pang’s laboratory.
Siti Aisyah Alias graduated from the University of Malaya, Malaysia with a BSc degree in Ecology in 1991 and earned a PhD degree in Marine Mycology at the University of Portsmouth in 1996. She is now a Researcher at the Institute of Ocean and Earth Sciences (IOES) and has been the Deputy Director of the National Antarctic Research Centre (NARC) and Malaysian Antarctic Research Programme (MARP). Her scientific research fields include fungal biodiversity and phylobiogeography and antimicrobial activity, cold-adapted enzymes and biochemistry of polar fungi.
Ali H. Bahkali obtained his PhD degree from the University of Wales, UK in 1983. He is working on various topics in mycology including ecology, physiology and molecular phylogeny.
Takahiko Nagahama is a well-known Researcher working on fungal diversity in the deep sea, especially marine yeasts. His research expertise includes molecular phylogeny, especially metagenomics.
Abdallah M. Elgorban is an Assistant Professor of plant pathology (mycology), with a BSc degree in Plant Pathology and MSc and PhD degrees of Plant Pathology (mycology). He has 15 years of academic experience at two major Arab universities (Mansoura University, Egypt and King Saud University, Saudi Arabia). He also has 17 years of experience in scientific research in the fields of plant pathology and marine mycology, with research interests in: morphological and molecular identification of plant-parasitic fungi and marine mycology; host/pathogen interactions; and biological control of plant-parasitic diseases.
Faten A. Abdel-Aziz obtained her MSc degree in Freshwater Fungi in 2001 and her PhD degree in aquatic fungi in 2004 from Sohag University, Egypt. She studies ecology, taxonomy and molecular phylogeny of aquatic fungi.
Mohamed S. Hodhod obtained his MSc degree from King Saud University, Saudi Arabia in 2012 on Marine Fungi under the supervision of Mohamed A. Abdel-Wahab.
Mohamed O. Al-Hebshi obtained his MSc degree from King Saud University, Saudi Arabia in 2014 in Marine Fungi under the supervision of Mohamed A. Abdel-Wahab.
Kevin D. Hyde is head of the Center of Excellence in Fungal Research. He obtained a PhD degree in Mycology from Portsmouth University in 1988 and a DSc from the University of Wales in 2001. He was one of the top 1% of cited scientists and listed as one as the most influential scientists in his field in the Thomson Reuters report in 2014. His current research includes mushroom growing, plant pathology and fungal ecology and phylogeny. Professor Hyde’s H’ Index in Web of Science is 62; he has published 950+ SCI papers and 20+ books and supervised about 100 PhD students.
Norlailatul A.B.M. Nor obtained her MSc degree from the University of Malaya in 2016 studying community structure of marine fungi on Nypa fruticans in Peninsular Malaysia under the supervision of Associate Prof. Dr. Siti Aisyah Alias. She worked as a Research Assistant in Dr. Siti Aisyah’s laboratory from 2009–2014.
Ka-Lai Pang obtained his BSc and PhD degrees from the City University of Hong Kong in 1998 and 2001, respectively. During 2002–2007, he worked as a Post-Doc Research Fellow at the University of Portsmouth (UK), BIOTEC (Thailand) and City University of Hong Kong on various projects related to the ecology and phylogeny of marine fungi. In 2007, he joined the faculty at National Taiwan Ocean University where he studies the biology of marine fungi and fungus-like organisms and endophytic fungi associated with mangrove plants and macroalgae.
E.B. Gareth Jones is a renowned Professor of Marine Mycology. He obtained his PhD degree from the University of Leeds, UK and was awarded a DSc from the University of Wales, UK. He has supervised over 100 PhD/MSc students and published over 500 research articles. He is now a Visiting Professor at the Chiang Mai University, Thailand.
K.L. Pang would like to thank the Ministry of Science and Technology, Taiwan for financial support (NSC101-2621-B-019-001-MY3). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for funding this research group (RG-1436-025). This work was financially supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training Grants BRT R_325015 and 352112. For their continued interest and support we also thank BIOTEC: Prof. Morakot Tanticharoen; Dr. Kanyawim Kirtikara; and Dr. Lily Eurwilaichitr.
Abdel-Wahab, M.A. 2011a. Lignicolous marine fungi from Yokosuka, Japan. Bot. Mar. 53: 283–289.10.1515/bot.2011.013Search in Google Scholar
Abdel-Wahab, M.A. 2011b. Marine fungi from Sarushima Island, Japan, with a phylogenetic evaluation of the genus Naufragella. Mycotaxon 115: 443–456.10.5248/115.443Search in Google Scholar
Abdel-Wahab, M.A. and A.H. Bahkali. 2012. Taxonomy of filamentous anamorphic marine fungi: morphology and molecular evidence. In: (E.B.G. Jones and K.L. Pang, eds) Marine fungi and fungal-like organisms. Walter de Gruyter, Berlin. pp. 65–90.10.1515/9783110264067.65Search in Google Scholar
Abdel-Wahab, M.A. and T. Nagahama. 2012. Halosarpheia japonica sp. nov. (Halosphaeriales, Ascomycota) from marine habitats in Japan. Mycol. Prog. 11: 85–92.10.1007/s11557-010-0731-0Search in Google Scholar
Acero, F.J., V. González, J. Sánchez-Ballesteros, V. Rubio, J. Checa, G.F. Bills, O. Salazar, G. Platas and F. Peláez. 2004. Molecular phylogenetic studies on the Diatrypaceae based on rDNA-ITS sequences. Mycologia 96: 249–259.10.2307/3762061Search in Google Scholar
Almeida, D.A.C., de, L.F.P. Gusmão and A.N. Miller. 2016. Taxonomy and molecular phylogeny of Diatrypaceae (Ascomycota, Xylariales) species from the Brazilian semi-arid region, including four new species. Mycol. Progr. 15: 53.10.1007/s11557-016-1194-8Search in Google Scholar
Badali, H., J. Chander, N. Gulati, A. Attri, R. Chopra, M.J. Najafzadeh, S. Chhabra, J.F.G.M. Meis and G.S. de Hoog. 2010. Subcutaneous phaeohyphomycotic cyst caused by Pyrenochaeta romeroi. Med. Biol. 48: 763–768.10.3109/13693780903440383Search in Google Scholar PubMed
Barghoorn, E.S. and D.H. Linder. 1944. Marine fungi: their taxonomy and biology. Farlowia 1: 395–467.10.5962/p.315987Search in Google Scholar
Bunyard, B.A., M.S. Nicholson and D.J. Royse. 1994. A systematic assessment of Morcella using RFLP analysis of the 28S ribosomal RNA gene. Mycologia 86: 762–772.10.2307/3760589Search in Google Scholar
Campbell, J., B. Volkmann-Kohlmeyer, T. Gräfenhan, J.W. Spataofora and J. Kohlmeyer. 2005. A reevaluation of Lulworthiales: relationships based on 18S and 28S rDNA. Mycol. Res. 109: 556–568.10.1017/S0953756205002716Search in Google Scholar
Carmarán, C.C., A.I. Romero and L.M. Giussani. 2006. An approach towards a new phylogenetic classification in Diatrypaceae. Fungal. Divers. 23: 67–87.Search in Google Scholar
Chu, Y., S.A. Alias, R. Idid, S.Y. Guo and K.L. Pang. 2015. A molecular reappraisal of Nimbospora (Halosphaeriaceae, Microascales) and a new genus Ebullia for N. octonae. Mycoscience 56: 34–41.10.1016/j.myc.2014.02.003Search in Google Scholar
Dayarathne, M.C., R. Phookamsak, K.D. Hyde, I.S. Manawasinghe, C. To-Anun and E.B.G. Jones. 2016. Halodiatrype, a novel diatrypaceous genus from mangroves with H. salinicola and H. avicenniae spp. nov. Mycosphere 7: 612–627.10.5943/mycosphere/7/5/7Search in Google Scholar
De Gruyter, J., J.H.C. Woudenberg, M.M. Aveskamp, G.J.M. Verkley, J.Z. Groenewald and P.W. Crous. 2012. Redisposition of Phoma–like anamorphs in Pleosporales. Stud. Mycol. 75: 1–36.10.3114/sim0004Search in Google Scholar
Glawe, D.A. and J.D. Rogers. 1984. Diatrypaceae in the Pacific Northwest. Mycotaxon 20: 401–460.Search in Google Scholar
Huelsenbeck, J.P. and F. Ronquist. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754–755.10.1093/bioinformatics/17.8.754Search in Google Scholar
Hyde, K.D. 1993a. Cryptovalsa halosarceicola sp. nov. an intertidal saprotroph of Halosarceia halocnemoides. Mycol. Res. 97: 799–800.10.1016/S0953-7562(09)81153-2Search in Google Scholar
Hyde, K.D. 1993b. Cryptosphaeria mangrovei sp. nov. (Diatrypaceae) from intertidal mangrove wood. Trans. Mycol. Soc. Jpn. 34: 311–314.Search in Google Scholar
Inderbitzin, P., M.A. Abdel-Wahab, E.B.G. Jones and L.L.P. Vrijmoed. 1999. A new species of Cryptovalsa from Mai Po mangrove in Hong Kong. Mycol. Res. 103: 1628–1630.10.1017/S0953756299001185Search in Google Scholar
Jones, E.B.G. 2011. Are there more marine fungi to be described? Bot. Mar. 54: 343–354.10.1515/bot.2011.043Search in Google Scholar
Jones, E.B.G. and A. Zainal. 1988. Ceriosporopsis caduca sp. nov., a marine lignicolous Ascomycotina from submerged wood. Mycotaxon 32: 237–244.Search in Google Scholar
Jones, E.B.G. and K.L. Pang. 2012. Tropical aquatic fungi. Biodivers. Conserv. 21: 2403–2423.10.1007/s10531-011-0198-6Search in Google Scholar
Jones, E.B.G., J. Sakayaroj, S. Suetrong, S. Somrithipol and K.L. Pang. 2009. Classification of marine Ascomycota, anamorphic taxa and Basidiomycota. Fungal Divers. 35: 1–187.Search in Google Scholar
Jones, E.B.G., S. Suetrong, J. Sakayaroj, A.H. Bahkali, M.A. Abdel-Wahab, T. Boekhout and K.L. Pang. 2015. Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota. Fungal. Divers. 73: 1–72.10.1007/s13225-015-0339-4Search in Google Scholar
Kirk, P.M., P.F. Cannon, D.W. Minter and J.A. Stalpers. 2008. Ainsworth & Bisby’s dictionary of the fungi, 10th edition. CABI, Wallingford. pp. 771.10.1079/9780851998268.0000Search in Google Scholar
Koch, J., K.L. Pang and E.B.G. Jones. 2007. Rostrupiella danica gen. et sp. nov., a Lulworthia-like marine lignicolous species from Denmark and the USA. Bot. Mar. 50: 294–301.10.1515/BOT.2007.034Search in Google Scholar
Kohlmeyer, J. 1981. Marine fungi from Martinique. Can. J. Bot. 59: 1314–1321.10.1139/b81-177Search in Google Scholar
Kohlmeyer, J. and E. Kohlmeyer. 1979. Marine mycology. The higher fungi. Academic Press, New York. pp. 690.Search in Google Scholar
Landvik, S. 1996. Neolecta, a fruit–body–producing genus of the basal ascomycetes, as shown by SSU and LSU rDNA sequence. Mycol. Res. 100: 199–202.10.1016/S0953-7562(96)80122-5Search in Google Scholar
Larget, B. and D.L. Simon. 1999. Markov Chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Mol. Biol. Evol. 16: 750–759.10.1093/oxfordjournals.molbev.a026160Search in Google Scholar
Liu, Y.J., S. Whelen and B.D. Hall. 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol. Biol. Evol. 16: 1799–1808.10.1093/oxfordjournals.molbev.a026092Search in Google Scholar PubMed
Maharachchikumbura, S.S.N., K.D. Hyde, E.B.G. Jones, E.H.C. McKenzie, J.D. Bhat, M.C. Dayarathne, S.K. Huang, C. Norphanphoun, I.C. Senanayake, R.H. Perera, Q.J. Shang, Y. Xiao, M.J. D’souza, S. Hongsanan, R.S. Jayawardena, D.A. Daranagama, S. Konta, I.D. Goonasekara, W.Y. Zhuang, R. Jeewon, A.J.L. Philllips, M.A. Abdel-Wahab, A.M. Al-Sadi, A.H. Bahkali, S. Boonmee, N. Boonyuen, R. Cheewangkoon, A.J. Dissanayake, J.C. Kang, Q.R. Li, J.K. Liu, X.Z. Liu, Z.Y. Liu, J.J. Luangsa-ard, K.L. Pang, R. Phooksamsak, I. Promputtha, S. Suetrong, M. Stadler, T.C. Wen and N.N. Wijayawardene. 2016. Families of Sordariomycetes. Fungal Divers. 79: 1–317.10.1007/s13225-016-0369-6Search in Google Scholar
Maharachchikumbura, S.S.N., K.D. Hyde, J.Z. Groenewald, J. Xu and P.W. Crous. 2014. Pestalotiopsis revisited. Stud Mycol. 79: 121–186.10.1016/j.simyco.2014.09.005Search in Google Scholar PubMed PubMed Central
Meyers, S.P. 1957. Taxonomy of marine Pyrenomycetes. Mycologia 49: 475–528.10.2307/3756156Search in Google Scholar
Miller, M.A., W. Pfeiffer and T. Schwartz. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010, New Orleans, LA. pp. 1–8.Search in Google Scholar
Nylander, J.A.A. 2004. MrModeltest v2. Evolutionary Biology Centre, Uppsala University, Norway.Search in Google Scholar
Pang, K.L. and J.S. Jheng. 2012. A checklist of marine fungi of Taiwan with a description of Kitesporella keelungensis gen. et sp. nov. Bot. Mar. 55: 459–466.10.1515/bot-2011-0092Search in Google Scholar
Rannala, B. and Z. Yang. 1996. Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J. Mol. Evol. 43: 304–311.10.1007/BF02338839Search in Google Scholar PubMed
Rappaz, F. 1987. Taxonomie et nomenclature des Diatrypacées à asques octosporées. Mycol. Helv. 2: 285–648.Search in Google Scholar
Réblová, M. 2011. New insights into the systematics and phylogeny of the genus Jattaea and similar fungi of the Calosphaeriales. Fungal Divers. 49: 167–198.10.1007/s13225-011-0099-8Search in Google Scholar
Sakayaroj, J., K.L. Pang and E.B.G. Jones. 2011. Multi-gene phylogeny of the Halosphaeriaceae: its ordinal status, relationships between genera and morphological character evolution. Fungal Divers. 46: 87–109.10.1007/s13225-010-0072-ySearch in Google Scholar
Spooner, B.M. 1981. New records and species of British microfungi. Trans. Br. Mycol. Soc. 76: 265–301.10.1016/S0007-1536(81)80149-0Search in Google Scholar
Stamatakis, A. 2006. Raxml-vi-hpc: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.10.1093/bioinformatics/btl446Search in Google Scholar
Stamatakis, A., P. Hoover and J. Rougemont. 2008. A rapid bootstrap algorithm for the RAxML Web servers. Syst. Biol. 57: 758–771.10.1080/10635150802429642Search in Google Scholar
Suetrong, S., A. Klaysuban, J. Sakayaroj, S. Preedanon, P. Ruang-Areerate, S. Phongpaichit, K.L. Pang and E.B.G. Jones. 2015. Tirisporellaceae, a new family in the order Diaporthales (Sordariomycetes, Ascomycota). Cryptogam. Mycol. 36: 1–12.10.7872/crym/v36.iss3.2015.319Search in Google Scholar
Swofford, D.L. 2002. PAUP* 4.0: phylogenetic analysis using parsimony (*and other methods). Sinauer, Sunderland, MA.Search in Google Scholar
Trouillas, F.P., W.M. Pitt, M.R. Sonsnowski, R. Huang, F. Peduto, A. Loschiavo, S. Savocchia, E.S. Scott and W.D. Gubler. 2011. Taxonomy and DNA phylogeny of Diatrypaceae associated with Vitis vinifera and other woody plants in Australia. Fungal Divers. 49: 203–223.10.1007/s13225-011-0094-0Search in Google Scholar
White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: (M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White TJ, eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, CA. pp. 315–322.Search in Google Scholar
Wilson, I.M. 1954. Ceriosporopsis halima Linder and Ceriosporopsis cambrensis sp. nov.: two marine pyrenomycetes on wood. Trans. Br. Mycol. Soc. 37: 272–285.10.1016/S0007-1536(54)80010-6Search in Google Scholar
Yusoff, M., J. Koch, E.B.G. Jones and S.T. Moss. 1993. Ultrastructural observations on a marine lignicolous ascomycete Bovicornua intricata gen. et sp. nov. Can. J. Bot. 71: 346–352.10.1139/b93-037Search in Google Scholar
Zhaxybayeva, O. and J.P. Gogarten. 2002. Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. BMC Genomics 3:4.10.1186/1471-2164-3-4Search in Google Scholar PubMed PubMed Central
©2017 Walter de Gruyter GmbH, Berlin/Boston