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Licensed Unlicensed Requires Authentication Published by De Gruyter May 5, 2022

Development and validation of microsatellite markers for Sargassum fusiforme based on transcriptomic data

  • Rujie Jia

    Rujie Jia is a graduate student jointly trained by Shanghai Ocean University (Shanghai) and Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences (Qingdao). Her research focus is on the genetic diversity of algal species.

    , Xiaoping Lu

    Xiaoping Lu works at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). Her research focuses on the protective adaptation mechanism of intertidal macroalgae to stress environment and the cultivation of superior strains of economic algae.

    , Wenjun Wang

    Wenjun Wang is a researcher at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). Her current research addresses the protective adaptation mechanism to stress environment, molecular development and generegulation, genetics breeding and cultivation techniques of macroalgae.

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    , Zhourui Liang

    Zhourui Liang is a researcher at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). His research focus is on seaweed biology and cultivation, including technology development for seedling rearing and cultivation as well as breeding of new cultivars with excellent performance.

    , Haiqin Yao

    Haiqin Yao is a postdoc in the Economic Seaweed Function Laboratory. She is investigating the Bangia atropurpurea and B. fuscopurpurea response to salinity stresses.

    and Baoxian Li
From the journal Botanica Marina

Abstract

The purpose of this study was to develop stable microsatellite markers and evaluate the genetic background of cultivated Sargassum fusiforme. Based on the transcriptome data obtained by high-throughput sequencing, eleven polymorphic microsatellite markers were developed using four S. fusiforme populations from China. One cultivated population was from Dongtou (DT) and three wild populations were from Muye Island (MY), Pingyu Island (PY) and Nanji Island (NJ). The cultivated S. fusiforme had the highest genetic diversity, with 90.91% polymorphic loci and Shannon’s information index (I) of 0.606, which were much higher than those of the wild populations (I = 0.425). The four populations were divided into two groups through a structure analysis. DT, PY and NJ were clustered into a group, and MY was an almost completely separate group. Both the structure and principal coordinates analysis showed that DT and PY had the lowest genetic differentiation, suggesting that the parents of the breeding population in Dongtou partly come from Pingyu Island. The observed heterozygosity of NJ was much higher than expected, indicating that the NJ wild resource was greatly affected by the environment. These eleven microsatellite markers could provide additional markers for germplasm resource evaluations and facilitate genetic analyses of the S. fusiforme population.


Corresponding author: Wenjun Wang, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Shinan District, Qingdao 266071, China, E-mail:
Rujie Jia and Xiaoping Lu Joint first authors.

Funding source: Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS

Award Identifier / Grant number: 20603022021008

Funding source: National Key R&D Program of China “Scientific and Technological Innovation of Blue Granary”

Award Identifier / Grant number: 2018YFD0901500

Award Identifier / Grant number: 2020TD27

Funding source: National Marine Genetic Resource Center

About the authors

Rujie Jia

Rujie Jia is a graduate student jointly trained by Shanghai Ocean University (Shanghai) and Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences (Qingdao). Her research focus is on the genetic diversity of algal species.

Xiaoping Lu

Xiaoping Lu works at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). Her research focuses on the protective adaptation mechanism of intertidal macroalgae to stress environment and the cultivation of superior strains of economic algae.

Wenjun Wang

Wenjun Wang is a researcher at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). Her current research addresses the protective adaptation mechanism to stress environment, molecular development and generegulation, genetics breeding and cultivation techniques of macroalgae.

Zhourui Liang

Zhourui Liang is a researcher at Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (Qingdao). His research focus is on seaweed biology and cultivation, including technology development for seedling rearing and cultivation as well as breeding of new cultivars with excellent performance.

Haiqin Yao

Haiqin Yao is a postdoc in the Economic Seaweed Function Laboratory. She is investigating the Bangia atropurpurea and B. fuscopurpurea response to salinity stresses.

  1. Research ethics: We confirm that we have included a statement specifying the local, national or international guidelines and legislation and the required or appropriate permissions and/or licences for the study.

  2. Author contribution: Rujie Jia: original concept, experimental design, laboratory work, data analysis, drafting and editing of manuscript; Xiaoping Lu: fieldwork and algae collection, experimental design, laboratory work, data analysis, drafting and editing of manuscript; Wenjun Wang: fieldwork and algae collection, editing of manuscript; Zhourui Liang: data analysis and editing of manuscript; Haiqin Yao: data analysis and editing of manuscript; Baoxian Li: data analysis and editing of manuscript.

  3. Research funding: This project was largely supported by the National Key R&D Program of China “Scientific and Technological Innovation of Blue Granary” (2018YFD0901500), National Marine Genetic Resource Centre, Central Public-interest Scientific Institution Basal Research Fund (2020TD27) and Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS (NO. 20603022021008).

  4. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

  5. Data availability: Sequence data of this project have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI) under the accession number PRJNA793347.

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Received: 2021-10-28
Revised: 2022-01-18
Accepted: 2022-03-30
Published Online: 2022-05-05
Published in Print: 2022-06-27

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