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Morphology, molecular phylogeny and toxicity of Fukuyoa paulensis (Dinophyceae) from the Bay of Bengal, North Indian Ocean

  • Oyeshina G. Oyeku

    Dr. Oyeshina G. Oyeku is a Pure and Applied Biology Programme lecturer in Bowen University, Iwo, Nigeria. His research focuses on the taxonomy, toxicity, and ecophysiology of harmful microalgae, bio-optic properties of microalgae, water quality, benthic macro-invertebrate ecology, biodiversity, conservation, and climate change.

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    and Subir K. Mandal

    Dr. Subir K. Mandal is a Principal Scientist at the CSIR-Central Salt and Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar, Gujarat, India. He researches algal taxonomy, ecology, toxicity, bio-optical properties and applications, water quality, heavy metals, climate change, as well as biodiversity and conservation.

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From the journal Botanica Marina

Abstract

Fukuyoa species are potential producers of polyether neurotoxins that are toxic to aquatic biota and humans. A strain of Fukuyoa (CSIRCSMCRI 004) was isolated from India’s Bay of Bengal coast. The clonal culture was identified using light and scanning electron microscopy and molecular phylogenetic analysis of the LSU rRNA gene and ITS (ITS1-5.8S gene-ITS2) sequences. Its toxicity was assessed using a 48 h Artemia bioassay. The morphological features observed included globular shape, descending cingular displacement, broad pentagonal 1′ plate, and large second apical plate (2′). The identity of the strain was confirmed to be F. paulensis based on the LSU rRNA gene and ITS sequences phylogenies. Strain CSIRCSMCRI 004 shared an identical sequence with the type strain of F. paulensis and other strains reported from different locations, based on the LSU rRNA gene and ITS sequences phylogenies. Exposure of brine shrimp larvae to its cellular lysate resulted in lethality (48 h LC50 = 0.256 mg ml−1) and behavioral abnormalities. The current study provides the first evidence of F. paulensis in the Bay of Bengal. It also reveals that CSIRCSMCRI 004 is toxic to susceptible biota like the brine shrimp and could produce ciguatera-associated toxins. The findings of this study highlight the need for sampling and screening of ciguatera-related species in the Bay of Bengal region to understand the associated public and environmental health risks.


Corresponding author: Oyeshina G. Oyeku, Division of Applied Phycology and Biotechnology, CSIR-Central Salt & Marine Chemicals Research Institute, Gijubhai Badheka Marg, 364002, Bhavnagar, Gujarat, India; and Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; and Pure and Applied Biology Programme, Bowen University, P. M. B 284, Iwo, Osun State, Nigeria, E-mail: ; and Subir K. Mandal, Division of Applied Phycology and Biotechnology, CSIR-Central Salt & Marine Chemicals Research Institute, Gijubhai Badheka Marg, 364002, Bhavnagar, Gujarat, India; and Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India, E-mail:

Funding source: CSIR-TWAS Postgraduate Fellowship

Award Identifier / Grant number: FR number: 3240293596

About the authors

Oyeshina G. Oyeku

Dr. Oyeshina G. Oyeku is a Pure and Applied Biology Programme lecturer in Bowen University, Iwo, Nigeria. His research focuses on the taxonomy, toxicity, and ecophysiology of harmful microalgae, bio-optic properties of microalgae, water quality, benthic macro-invertebrate ecology, biodiversity, conservation, and climate change.

Subir K. Mandal

Dr. Subir K. Mandal is a Principal Scientist at the CSIR-Central Salt and Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar, Gujarat, India. He researches algal taxonomy, ecology, toxicity, bio-optical properties and applications, water quality, heavy metals, climate change, as well as biodiversity and conservation.

Acknowledgments

The authors thank the Council of Scientific and Industrial Research (CSIR), India, and The World Academy of Science (TWAS), Trieste, Italy, fellowship for providing funding for Ph.D. research and study. This manuscript is assigned the CSIR-CSMCRI contribution number 113/2022.

  1. Research ethics: The procedures undertaken in this study were by national laws.

  2. Author contributions: O.G. Oyeku: conceptualization, methodology, investigation, data curation, formal analysis, visualization, writing – original draft, writing – review and editing. S.K. Mandal: conceptualization, supervision, writing – review, and editing.

  3. Competing interests: The authors declare no known conflicts of interest regarding this article.

  4. Research funding: O.G. Oyeku received a scholarship and fellowship from the Council of Scientific and Industrial Research (CSIR), India, and The World Academy of Science (TWAS), Trieste, Italy (FR number: 3240293596).

  5. Data availability: The LSU and ITS rRNA gene sequences obtained in this study have been deposited in GenBank with accession nos. MW077867 and MW077862, respectively.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/bot-2023-0023).


Received: 2023-04-04
Accepted: 2023-11-07
Published Online: 2023-12-05
Published in Print: 2024-02-26

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