Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter August 19, 2012

Performance evaluation of three different immunoassays for detection of antibodies to hepatitis B core

Lou Bin, Fan Jian, Yuan Quan, Tan Yajun, Tian Li, Zhang Jie and Chen Yu

Corresponding author: Chen Yu, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, Zhejiang Province 310003, P.R. China, Phone: +86 571 87233001, Fax: +86 571 87236383

The study was approved by the Ethical Review Board of the First Affiliated Hospital, College of Medicine, Zhejiang University. We thank all the participants for collecting data and testing specimens, and the National Institute of Diagnostics and Vaccine Development in Infectious Diseases for support in this project.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.

Research funding: National 863 Program for the Biological and medical technology (no. 2011AA02A100) and the National Key Programs for Infectious Diseases of China (no. 2012ZX10002005.

Employment or leadership: None declared.

Honorarium: None declared.

References

1. Roth WK, Weber M, Petersen D, Drosten C, Buhr S, Sireis W, et al. NAT for HBV and anti-HBc testing increase blood safety. Transfusion 2002;42:869–75.1237565910.1046/j.1537-2995.2002.00128.xSearch in Google Scholar

2. Arraes LC, Ximenes R, Andrieu JM, Lu W, Barreto S, Pereira LM, et al. The biological meaning of anti-HBC positive result in blood donors: relation to HBV-DNA and to other serological markers. Rev Inst Med Trop São Paulo 2003;45:137–40.1287006210.1590/S0036-46652003000300004Search in Google Scholar

3. Hoofnagle JH, Seeff LB, Bales ZB, Zimmerman HJ. Type B hepatitis after transfusion with blood containing antibody to hepatitis B core antigen. N Engl J Med 1978;298:1379–83.10.1056/NEJM197806222982502Search in Google Scholar

4. Vitale F, Tramuto F, Orlando A, Vizzini G, Meli V, Cerame G, et al. Can the serological status of anti-HBc alone be considered a sentinel marker for detection of occult HBV infection? J Med Virol 2008;80:577–82.1829770710.1002/jmv.21121Search in Google Scholar

5. Yuan Q, Ou SH, Chen CR, Ge SX, Pei B, Chen QR, et al. Molecular characteristics of occult hepatitis B virus from blood donors in southeast. China J Clin Microbiol 2010;48:357–62.10.1128/JCM.01781-09Search in Google Scholar

6. Li A, Yuan Q, Huang Z, Fan J, Guo R, Lou B, et al. Novel double-antigen sandwich immunoassay for human hepatitis B core antibody. Clin Vaccine Immunol 2010; 17:464–9.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000275056400020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f32010700810.1128/CVI.00457-09Search in Google Scholar

7. Caspari G, Beyer HJ, Elbert G, Koerner K, Muss P, Schunter FW, et al. Unsatisfactory specificities and sensitivities of six enzyme immunoassays for antibodies to hepatitis B core antigen. J Clin Microbiol 1989;27: 2067–72.267419910.1128/jcm.27.9.2067-2072.1989Search in Google Scholar

8. Deng LJ, Xu Y, Huang J. Developing a double-antigen sandwich ELISA for effective detection of human hepatitis B core antibody. Comp Immunol Microbiol Infect Dis 2008;31:515–26.18242704http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000261669400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f3Search in Google Scholar

9. Liang XF, Bi SL, Yang WZ, Wang LD, Cui G, Cui FQ, et al. Epidemiological serosurvey of hepatitis B in China–declining HBV prevalence due to hepatitis B vaccination. Vaccine 2009;27:6550–7.10.1016/j.vaccine.2009.08.048http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000272056300009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f319729084Search in Google Scholar

Received: 2012-02-15
Accepted: 2012-07-10
Published Online: 2012-08-19
Published in Print: 2013-02-01

©2013 by Walter de Gruyter Berlin Boston