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Licensed Unlicensed Requires Authentication Published by De Gruyter August 7, 2014

Performance evaluation of the digital cell imaging analyzer DI-60 integrated into the fully automated Sysmex XN hematology analyzer system

  • Yoko Tabe EMAIL logo , Takemasa Yamamoto , Imiko Maenou , Rie Nakai , Mayumi Idei , Takashi Horii , Takashi Miida and Akimichi Ohsaka

Abstract

Background: The XN-Series (Sysmex, Kobe, Japan) have been equipped with the automated digital cell imaging analyzer DI-60, which provides complete automation of the sample processing with automated complete blood counts (CBC), slide making/staining, and digital scanning with cell pre-classification. The aim of this study was to evaluate the efficacy of the XN-Series as an integrated blood cell analysis system.

Methods: White blood cell (WBC) morphological analysis by the DI-60 was evaluated using 232 blood samples from patients. Routine analysis of a total of 2000 blood samples has been performed to evaluate the processing ability of the XN-Series connected to the DI-60.

Results: The overall analysis accuracy of pre-classification of WBC by the DI-60 was 88.4%. Good correlation was observed between final results of the DI-60 analysis and manual differentiation with high sensitivity and specificity for blasts and immature granulocytes. The sample processing time of the XN-Series, from automated CBC to cell pre-classification, was 38±1 min/single run and 165±12 min/500 CBC samples run (slide preparation rate 15.6%) with no sample hold-up at the DI-60.

Conclusions: The automated morphological analysis capability of the DI-60 has potential usefulness in the integrated automated hematology analysis system of XN-Series.


Corresponding author: Yoko Tabe, MD, PhD, Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan, Phone: +81-3-3813-3111 ext. 5187, Fax: +81-3-5804-8637, E-mail:

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Received: 2014-4-24
Accepted: 2014-7-8
Published Online: 2014-8-7
Published in Print: 2015-2-1

©2015 by De Gruyter

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