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Licensed Unlicensed Requires Authentication Published by De Gruyter December 2, 2014

Reference interval for immature platelet fraction on Sysmex XN hematology analyzer: a comparison study with Sysmex XE-2100

Young Jin Ko, Mina Hur, Hanah Kim, Sang Gyeu Choi, Hee-Won Moon and Yeo-Min Yun

Abstract

Background: Recently introduced hematology analyzer, the Sysmex XN modular system (Sysmex, Kobe, Japan), has newly adopted a florescent channel to detect platelets and immature platelet fraction (IPF). This study aimed to establish new reference intervals for %-IPF and absolute number of IPF (A-IPF) on Sysmex XN. Platelet counts, %-IPF, and A-IPF were also compared between Sysmex XN and XE-2100 systems (Sysmex).

Methods: Except outliers, blood samples from 2104 healthy individuals and 140 umbilical cord blood were analyzed using both Sysmex XN and XE-2100. The results of two systems were compared using Bland-Altman plot. The reference intervals for %-IPF and A-IPF were defined using non-parametric percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3).

Results: The platelet counts, %-IPF, and A-IPF showed non-parametric distributions. The mean difference between Sysmex XN and XE-2100 in healthy individuals revealed a positive bias in platelets (+8.0×109/L), %-IPF (+1.2%), and A-IPF (+3.0×109/L). The reference intervals for %-IPF and A-IPF on Sysmex XN were: 1.0%–7.3% and 2.49–15.64×109/L in healthy individuals; and 1.0%–4.4% and 2.94–12.82×109/L in umbilical cord blood.

Conclusions: This large-scale study demonstrates a clear difference of platelet counts and IPF between Sysmex XN and XE-2100. The new reference intervals for IPF on Sysmex XN would provide fundamental data for clinical practice and future research.


Corresponding author: Mina Hur, Department of Laboratory Medicine, Konkuk University School of Medicine, Konkuk University Hospital, 120-1, Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul 143-729, Republic of Korea, Phone: +82 2 2030 5581, Fax: +82 2 2636 6764, E-mail:

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Received: 2014-8-20
Accepted: 2014-10-29
Published Online: 2014-12-2
Published in Print: 2015-6-1

©2015 by De Gruyter

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