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Licensed Unlicensed Requires Authentication Published by De Gruyter June 6, 2015

Comparison of capillary electrophoresis and high performance liquid chromatography for detection and quantification of hemoglobin New York

  • Li You-Qiong , Huang Hui-Ping , Chen Zhi-Zhong , Zhao Lin , Liang Liang , Qin Gui-Fang and Mo Yun EMAIL logo

Abstract

Background: Hemoglobin (Hb) New York [β113 (G15) Val→Glu, GTG→GAG], also known as Hb Kaohsiung, is one of the most common Hb variants in South China. Currently, most used screening methods for hemoglobinopathies in South China are high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). However, there is no study comparing the performance of CE and HPLC in the detection of Hb New York.

Methods: In total 15 samples (including 13 adult blood samples and 2 cord blood samples) with heterozygous Hb New York were analyzed by CE and HPLC. Levels of Hb New York, HbA2, Hb, mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were compared within the two groups.

Results: All 15 cases (100%) were detected by CE, whereas none was detected by HPLC. Mean values of Hb New York and HbA2 in adult heterozygous group were (43.82±2.47)% and (2.85±0.31)%, respectively; mean values for cord blood group were (7.95±0.78)% and (0.30±0.14)%.

Conclusions: CE allows the detection of Hb New York, while this variant is not separated from Hb A on HPLC. CE may be the preferred method for hemoglobinopathy screening in areas with high prevalence of Hb New York.


Corresponding author: Prof. Mo Yun, Department of Obstetrics and Gynecology, People’s Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning 530021, P.R. China, Phone: +86 0771 2186127, E-mail:

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2015-3-8
Accepted: 2015-4-27
Published Online: 2015-6-6
Published in Print: 2016-1-1

©2016 by De Gruyter

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