Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter December 17, 2015

Differences in analytical and biological results between older and newer lots of a widely used irisin immunoassay question the validity of previous studies

  • Rafael Montes-Nieto , M. Ángeles Martínez-García , Manuel Luque-Ramírez and Héctor F. Escobar-Morreale EMAIL logo

Corresponding author: Héctor F. Escobar-Morreale, Hospital Universitario Ramón y Cajal, Carretera de Colmenar km 9’1, E-28034 Madrid, Spain, Phone/Fax: +34-91 336 9029, E-mail:


1. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-[agr]-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012;481:463–8.10.1038/nature10777Search in Google Scholar PubMed PubMed Central

2. Jedrychowski MP, Wrann CD, Paulo JA, Gerber KK, Szpyt J, Robinson MM, et al. Detection and quantitation of circulating human irisin by tandem mass spectrometry. Cell Metab 2015;22:734–40.10.1016/j.cmet.2015.08.001Search in Google Scholar PubMed PubMed Central

3. Sanchis-Gomar F, Alis R, Lippi G. Circulating irisin detection: does it really work? Trends Endocrinol Metab 2015;26:335–6.10.1016/j.tem.2015.05.004Search in Google Scholar PubMed

4. Sanchis-Gomar F, Alis R, Pareja-Galeano H, Romagnoli M, Perez-Quilis C. Inconsistency in circulating irisin levels: what is really happening? Horm Metab Res 2014;46:591–6.10.1055/s-0033-1363283Search in Google Scholar PubMed

5. Crujeiras AB, Pardo M, Casanueva FF. Irisin: ‘fat’ or artefact. Clinical Endocrinology 2015;82:467–74.10.1111/cen.12627Search in Google Scholar PubMed

6. Albrecht E, Norheim F, Thiede B, Holen T, Ohashi T, Schering L, et al. Irisin – a myth rather than an exercise-inducible myokine. Sci Rep 2015;5:8889.10.1038/srep08889Search in Google Scholar PubMed PubMed Central

7. Erickson HP. Irisin and FNDC5 in retrospect: an exercise hormone or a transmembrane receptor? Adipocyte 2013;2:289–93.10.4161/adip.26082Search in Google Scholar PubMed PubMed Central

8. Tang S, Zhang R, Jiang F, Wang J, Chen M, Peng D, et al. Circulating irisin levels are associated with lipid and uric acid metabolism in a Chinese population. Clin Exp Pharmacol Physiol 2015;42:896–901.10.1111/1440-1681.12439Search in Google Scholar PubMed

Received: 2015-11-2
Accepted: 2015-11-11
Published Online: 2015-12-17
Published in Print: 2016-7-1

©2016 by De Gruyter

Downloaded on 21.3.2023 from
Scroll Up Arrow