Acknowledgments
We thank the laboratory technicians of the Laboratory Hematology Unit of the Pathology Department of Centro Hospitalar Universitário do Porto, Conceição Valente and Luísa Lage, for their valuable technical support. We also thank the clinical and technical staff of Department of Clinical Hematology of Centro Hospitalar e Universitário de Coimbra for their assistance performing molecular testing.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Edward HL, Pisani LA, Rodriguez-Romero WE, Chaves-Villalobos J, Garcia-Quesada J, Harris NS, et al. Hb Youngstown [β101(G3)Glu → Ala; HBB: c.305A>C]: An unstable hemoglobin variant causing severe hemolytic anemia. Hemoglobin 2014;38:381–4.10.3109/03630269.2014.971960Search in Google Scholar PubMed
2. Bain BJ. Blood cells: a practical guide, 5th ed. Oxford: Wiley-Blackwell, 2015:322–3.Search in Google Scholar
3. Yates AM, Mortier NA, Hyde KS, Hankins JS, Ware RE. The diagnostic dilemma of congenital unstable hemoglobinopathies. Pediatr Blood Cancer 2010;55:1393–5.10.1002/pbc.22702Search in Google Scholar PubMed PubMed Central
4. Kumar MK, Judd C, Hoyer JD, Swanson KC, Nelson L, Oliveira JL. Hb Manukau [β67(E11)Val → Gly; HBB: c.203T>G]: the role of genetic testing in the diagnosis of idiopathic hemolytic anemia. Hemoglobin 2014;38:211–2.10.3109/03630269.2014.895378Search in Google Scholar PubMed
5. Mongelli F, Barberio G, Ivaldi G. A rare and unstable hemoglobin variant, Hb M Dothan β 25/26 (-GTG), detected by the anomalous cytogram on Sysmex XE-2100. Clin Chem Lab Med 2016;54:e31–3.10.1515/cclm-2015-0566Search in Google Scholar PubMed
6. Rosetti M, Poletti G, Sensi A, Ravani A, Rondoni M, Baldrati L, et al. A rare case of Hemoglobin Leiden interfering with the DIFF channel of Sysmex XE-2100. Scand J Clin Lab Invest 2015;75:436–7.10.3109/00365513.2015.1033743Search in Google Scholar PubMed
7. Inoue H. Overview of automated hematology analyzer XE-2100. Sysmex J Int 1999;9:58–64.Search in Google Scholar
8. Harris N, Kunicka J, Kratz A. The ADVIA 2120 Hematology System: Flow Cytometry-Based Analysis of Blood and Body Fluids in the Routine Hematology Laboratory. Lab Hematol 2005;11:47–61.10.1532/LH96.04075Search in Google Scholar PubMed
9. Briggs C, Culp N, Davis B, D’Onofrio G, Zini G, Machin SJ, et al. ICSH guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting. Int J Lab Hematol 2014;36:613–27.10.1111/ijlh.12201Search in Google Scholar PubMed
©2017 Walter de Gruyter GmbH, Berlin/Boston
