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Licensed Unlicensed Requires Authentication Published by De Gruyter January 8, 2018

Is the DNA of placental origin packaged in exosomes isolated from plasma and serum of pregnant women?

Gabriela Repiská, Barbora Konečná, Ganesh V. Shelke, Cecilia Lässer, Barbora Izrael Vlková and Gabriel Minárik

Corresponding author: Mgr. Gabriela Repiská, PhD, Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 813 72 Bratislava, Slovakia

  1. Authors contributions: All the authors have accepted responsibility for entire content of this submitted manuscript and approved submission.

  2. Research funding: The study was supported by Grant Agency of Ministry of Education, Science, Research and Sport of the Slovak Republic (VEGA-1/0064/17) and by funding from the Comenius University in Bratislava, Slovakia (UK/102/2015).

  3. Employed or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interest: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007;9:654–9.Search in Google Scholar

2. Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol 2006;Chapter 3: Unit 3 22.Search in Google Scholar

3. Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles 2015;4:27031.Search in Google Scholar

4. Repiska G, Sedlackova T, Szemes T, Celec P, Minarik G. Selection of the optimal manual method of cell free fetal DNA isolation from maternal plasma. Clin Chem Lab Med 2013;51:1185–9.Search in Google Scholar

5. Wong FC, Sun K, Jiang P, Cheng YK, Chan KC, Leung TY, et al. Cell-free DNA in maternal plasma and serum: a comparison of quantity, quality and tissue origin using genomic and epigenomic approaches. Clin Biochem 2016;49:1379–86.Search in Google Scholar

6. Lui YY, Chik KW, Chiu RW, Ho CY, Lam CW, Lo YM. Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation. Clin Chem 2002;48:421–7.Search in Google Scholar

7. Manokhina I, Singh TK, Penaherrera MS, Robinson WP. Quantification of cell-free DNA in normal and complicated pregnancies: overcoming biological and technical issues. PLoS One 2014;9:e101500.Search in Google Scholar

8. Shelke GV, Jang SC, Yin Y, Lasser C, Lotvall J. Human mast cells release extracellular vesicle-associated DNA. Matters 2016;2:e201602000034.Search in Google Scholar

9. Lazaro-Ibanez E, Sanz-Garcia A, Visakorpi T, Escobedo-Lucea C, Siljander P, Ayuso-Sacido A, et al. Different gDNA content in the subpopulations of prostate cancer extracellular vesicles: apoptotic bodies, microvesicles, and exosomes. Prostate 2014;74:1379–90.Search in Google Scholar

10. Mitchell MD, Peiris HN, Kobayashi M, Koh YQ, Duncombe G, Illanes SE, et al. Placental exosomes in normal and complicated pregnancy. Am J Obstet Gynecol 2015;213:S173–81.Search in Google Scholar

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Received: 2017-6-28
Accepted: 2017-12-6
Published Online: 2018-1-8
Published in Print: 2018-5-24

©2018 Walter de Gruyter GmbH, Berlin/Boston