Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter November 8, 2017

Impact of citrated blood collection tubes on red cell morphology: implications for the measurement of plasma glucose

  • Helen Lunt EMAIL logo , Helen F. Heenan , Peter Edwards and Christopher M. Florkowski
  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: New Zealand Society for the Study of Diabetes, (Grant/Award Number: ‘NZSSD award 2015’).

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Uchida K, Okuda S, Tanaka K. Method of inhibiting glycolysis in blood samples. US Patent 4,780,419. 1988 Oct 4. 20. Available at: Accessed: 5 Sep 2017.Search in Google Scholar

2. Saracevic A, Dukic L, Juricic G, Milevoj Kopcinovic L, Mirosevic G, Simundic AM. Various glycolysis inhibitor-containing tubes for glucose measurement cannot be used interchangeably due to clinically unacceptable biases between them. Clin Chem Lab Med 2018;56:236–41.10.1515/cclm-2017-0279Search in Google Scholar

3. Venosafe glycaemia tube with unique FC-mixture. Available at: Accessed: 5 Sep 2017.Search in Google Scholar

4. Weed RI, Chailley B. Calcium-pH interactions in the production of shape change in erythrocytes. Nouv Rev Fr Hémat 1972;12: 775–88.10.1007/978-3-642-88062-9_8Search in Google Scholar

5. Gros M, Vrhovec S, Brumen M, Svetina S, Zeks B. Low pH induced shape changes and vesiculation of human erythrocytes. Gen Physiol Biophys 1996;15:145–63.Search in Google Scholar

6. Gedde MM, Yang E, Huestis WH. Resolution of the paradox of red cell shape changes in low and high pH. Biochim Biophys Acta 1999;1417:246–53.10.1016/S0005-2736(99)00007-3Search in Google Scholar

7. Rudenko SV. Erythrocyte morphological states, phases, transitions and trajectories. Biochimica et Biophysica Acta (BBA) – Biomembranes 2010;1798:1767–78.10.1016/j.bbamem.2010.05.010Search in Google Scholar PubMed

8. Carey R, Lunt H, Heenan HF, Frampton CM, Florkowski CM. Collection tubes containing citrate stabiliser over-estimate plasma glucose, when compared to other samples undergoing immediate plasma separation. Clin Biochem 2016;18: 1406–11.10.1016/j.clinbiochem.2016.05.017Search in Google Scholar PubMed

Received: 2017-9-4
Accepted: 2017-10-4
Published Online: 2017-11-8
Published in Print: 2018-3-28

©2018 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 1.4.2023 from
Scroll to top button