Accessible Requires Authentication Published by De Gruyter May 1, 2018

Unstable haemoglobin variant Hb Leiden is detected on Sysmex XN-Series analysers

Wesley Jongbloed, Gitte van Twillert, Margreet Schoorl and Roger K. Schindhelm


The authors thank Dr. C.L. Harteveld of the Haemoglobinopathies Laboratory in Leiden, The Netherlands, for performing the DNA analysis. The patient consented in publishing this case report.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Edward HL, Pisani LA, Rodriguez-Romero WE, Chaves-Villalobos J, Garcia-Quesada J, Harris NS, et al. Hb Youngstown (beta101(G3)Glu→Ala; HBB: c.305A>C: an unstable hemoglobin variant causing severe hemolytic anemia. Hemoglobin 2014;38:381–4. Search in Google Scholar

2. Teixeira C, Pina D, Freitas MI. Automated detection of unstable hemoglobin variants by Sysmex XE-Series analyzers. Clin Chem Lab Med 2017;55:e243–6. Search in Google Scholar

3. Rosetti M, Poletti G, Sensi A, Ravani A, Rondoni M, Baldrati L, et al. A rare case of Hemoglobin Leiden interfering with the DIFF channel of Sysmex XE-2100. Scand J Clin Lab Invest 2015;75:436–7. Search in Google Scholar

4. Mongelli F, Barberio G, Ivaldi G. A rare and unstable hemoglobin variant, Hb M Dothan beta 25/26 (-GTG), detected by the anomalous cytogram on Sysmex XE-2100. Clin Chem Lab Med 2016;54:e31–3. Search in Google Scholar

5. Briggs C, Longair I, Kumar P, Singh D, Machin SJ. Performance evaluation of the Sysmex haematology XN modular system. J Clin Pathol 2012;65:1024–30. Search in Google Scholar

6. Schoorl M, Schoorl M, Chevallier M, Elout J, van Pelt J. Flagging performance of the Sysmex XN2000 haematology analyser. Int J Lab Hem 2016;38:160–6. Search in Google Scholar

Received: 2018-01-12
Accepted: 2018-03-06
Published Online: 2018-05-01
Published in Print: 2018-08-28

©2018 Walter de Gruyter GmbH, Berlin/Boston