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Reference values for 24,25-dihydroxyvitamin D and the 25-hydroxyvitamin D/24,25-dihydroxyvitamin D ratio

Niek F. Dirks, Mariëtte T. Ackermans, Robert de Jonge and Annemieke C. Heijboer

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Dirks NF, Ackermans MT, Lips P, de Jongh RT, Vervloet MG, de Jonge R, et al. The when, what & how of measuring vitamin D metabolism in clinical medicine. Nutrients 2018;10:482. Search in Google Scholar

2. Schlingmann KP, Kaufmann M, Weber S, Irwin A, Goos C, John U, et al. Mutations in CYP24A1 and idiopathic infantile hypercalcemia. N Engl J Med 2011;365:410–21. Search in Google Scholar

3. Ketha H, Kumar R, Singh RJ. LC-MS/MS for identifying patients with CYP24A1 mutations. Clin Chem 2016;62:236–42. Search in Google Scholar

4. Nesterova G, Malicdan MC, Yasuda K, Sakaki T, Vilboux T, Ciccone C, et al. 1,25-(OH)2D-24 hydroxylase (CYP24A1) deficiency as a cause of nephrolithiasis. Clin J Am Soc Nephrol 2013;8:649–57. Search in Google Scholar

5. Food and Drug Administration. Bioanalytical Method Validation. Guidance for Industry. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Biologics Evaluation and Research, Center for Veterinary Medicine, 2018. Search in Google Scholar

6. European Medicines Agency. EMEA/CHMP/EWP/192217/2009 Rev. 1 Corr. 2 -Guideline on Bioanalytical Method Validation, 2011. Search in Google Scholar

7. Wise SA, Tai SS, Nelson MA, Burdette CQ, Camara JE, Hoofnagle AN, et al. Interlaboratory comparison for the determination of 24,25-dihydroxyvitamin D(3) in human serum using liquid chromatography with tandem mass spectrometry. J AOAC Int 2017;100:1308–17. Search in Google Scholar

8. Tang JC, Nicholls H, Piec I, Washbourne CJ, Dutton JJ, Jackson S, et al. Reference intervals for serum 24,25-dihydroxyvitamin D and the ratio with 25-hydroxyvitamin D established using a newly developed LC-MS/MS method. J Nutr Biochem 2017;46:21–9. Search in Google Scholar

Supplementary Material

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Received: 2018-10-09
Accepted: 2019-03-04
Published Online: 2019-03-23
Published in Print: 2019-09-25

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