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Licensed Unlicensed Requires Authentication Published by De Gruyter March 23, 2019

Erroneous thyroid and steroid hormones profile due to anti-streptavidin antibodies

Jean-Louis Bayart , Julien Favresse , Ekaterina Melnik , Benjamin Lardinois , Catherine Fillée , Dominique Maiter and Pr. Damien Gruson EMAIL logo

Corresponding author: Pr. Damien Gruson, Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St. Luc – Université Catholique de Louvain, Tour Claude Bernard, 54 Avenue Hippocrate, 1200 Brussels, Belgium; and Département des laboratoires cliniques, Cliniques Universitaires St. Luc – Université Catholique de Louvain, Brussels, Belgium, Phone: +32-(0)2-7646747, Fax: +32-(0)2-7646930

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


1. Clerico A, Belloni L, Carrozza C, Correale M, Dittadi R, Dotti C, et al. A Black Swan in clinical laboratory practice: the analytical error due to interferences in immunoassay methods. Clin Chem Lab Med 2018;56:397–402.10.1515/cclm-2017-0881Search in Google Scholar

2. Favresse J, Burlacu M-C, Maiter D, Gruson D. Interferences with thyroid function immunoassays: clinical implications and detection algorithm. Endocr Rev 2018;39:830–50.10.1210/er.2018-00119Search in Google Scholar

3. Leslie H, Courtney C, Bell P, Hadden D, McCance D, Ellis P, et al. Laboratory and clinical experience in 55 patients with macroprolactinemia identified by a simple polyethylene glycol precipitation method. J Clin Endocrinol Metab 2001;86:2743–6.10.1210/jcem.86.6.7521Search in Google Scholar

4. Langlois F, Moramarco J, He G, Carr BR. Falsely elevated steroid hormones in a postmenopausal woman due to laboratory interference. J Endocr Soc 2017;1:1062–6.10.1210/js.2017-00191Search in Google Scholar

5. Bjerner J, Nustad K, Norum LF, Olsen KH, Børmer OP. Immunometric assay interference: incidence and prevention. Clin Chem 2002;48:613–21.10.1093/clinchem/48.4.613Search in Google Scholar

6. Piketty M-L, Prie D, Sedel F, Bernard D, Hercend C, Chanson P, et al. High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference. Clin Chem Lab Med 2017;55:817–25.10.1515/cclm-2016-1183Search in Google Scholar

7. Berth M, Willaert S, De Ridder C. Anti-streptavidin IgG antibody interference in anti-cyclic citrullinated peptide (CCP) IgG antibody assays is a rare but important cause of false-positive anti-CCP results. Clin Chem Lab Med 2018;56:1263–8.10.1515/cclm-2017-1153Search in Google Scholar

8. Lam L, Bagg W, Smith G, Chiu W, Middleditch MJ, Lim JC-H, et al. Apparent hyperthyroidism caused by biotin-like interference from IgM anti-streptavidin antibodies. Thyroid 2018;28:1063–7.10.1089/thy.2017.0673Search in Google Scholar

9. Peltier L, Massart C, Moineau M-P, Delhostal A, Roudaut N. Anti-streptavidin interferences in Roche thyroid immunoassays: a case report. Clin Chem Lab Med 2016;54:e11–4.10.1515/cclm-2015-0350Search in Google Scholar

10. Rulander NJ, Cardamone D, Senior M, Snyder PJ, Master SR. Interference from anti-streptavidin antibody. Arch Pathol Lab Med 2013;137:1141–6.10.5858/arpa.2012-0270-CRSearch in Google Scholar

Received: 2018-12-20
Accepted: 2019-02-21
Published Online: 2019-03-23
Published in Print: 2019-09-25

©2019 Walter de Gruyter GmbH, Berlin/Boston

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