Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 7, 2019

An approach based on simulated hemolysis for establishing the hemolysis index threshold for high-sensitivity cardiac troponin T assay

Francesca Trimboli, Fortunata Lucia, Elvira Angotti, Giulio Cesare Antico, Lorenzina Carinci Giacquinto, Maria Martucci, Serafina Mancuso, Roberta Chirillo, Domenico Britti, Giovanni Cuda, Francesco Costanzo and Camillo Palmieri

Corresponding authors: Prof. Giovanni Cuda, U.O. of Clinical Biochemistry–AOU Materdomini Hospital, Catanzaro, Italy; and Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy; and Prof. Camillo Palmieri, U.O. of Clinical Biochemistry–AOU Materdomini Hospital, Catanzaro, Italy; and Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy, Phone: +39-0961-3695181, Fax: +39-0961-3694090
aCo-last authors.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. Florkowski C, Wallace J, Walmsley T, George P. The effect of hemolysis on current troponin assays – a confounding preanalytical variable? Clin Chem 2010;56:1195–7.10.1373/clinchem.2009.140863Search in Google Scholar

2. Li A, Brattsand G. Stability of serum samples and hemolysis interference on the high sensitivity troponin T assay. Clin Chem Lab Med 2011;49:335–6.10.1515/CCLM.2011.035Search in Google Scholar

3. Monneret D, Mestari F, Atlan G, Corlouer C, Ramani Z, Jaffre J, et al. Hemolysis indexes for biochemical tests and immunoassays on Roche analyzers: determination of allowable interference limits according to different calculation methods. Scand J Clin Lab Invest 2015;75:162–9.10.3109/00365513.2014.993691Search in Google Scholar

4. Farrell CJ, Carter AC. Hemolysis interference: are laboratories getting the information they need? Clin Chem 2016;62:1274–6.10.1373/clinchem.2016.258277Search in Google Scholar

5. Petrova DT, Cocisiu GA, Eberle C, Rhode KH, Brandhorst G, Walson PD, et al. Can the Roche hemolysis index be used for automated determination of cell-free hemoglobin? A comparison to photometric assays. Clin Biochem 2013;46:1298–301.10.1016/j.clinbiochem.2013.06.018Search in Google Scholar

6. Dolci A, Panteghini M. Harmonization of automated hemolysis index assessment and use: is it possible? Clin Chim Acta 2014;432:38–43.10.1016/j.cca.2013.10.012Search in Google Scholar

7. Lippi G. Systematic assessment of the hemolysis index: pros and cons. Adv Clin Chem 2015;71:157–70.10.1016/bs.acc.2015.05.002Search in Google Scholar

8. Wu AH, Christenson RH, Greene DN, Jaffe AS, Kavsak PA, Ordonez-Llanos J, et al. Clinical laboratory practice recommendations for the use of cardiac troponin in acute coronary syndrome: expert opinion from the Academy of the AmericanAssociation for Clinical Chemistry and the task force on clinical applications of cardiac bio-markers of the International Federation of Clinical Chemistry and Laboratory Medicine. Clin Chem 2018;64:645–55.10.1373/clinchem.2017.277186Search in Google Scholar

9. Frankenstein L, Wu AH, Hallermayer K, Wians FH, Jr., Giannitsis E, Katus HA. Biological variation and reference change value of high-sensitivity troponin T in healthy individuals during short and intermediate follow-up periods. Clin Chem 2011;57:1068–71.10.1373/clinchem.2010.158964Search in Google Scholar

10. Ceriotti F, Fernandez-Calle P, Klee GG, Nordin G, Sandberg S, Streichert T, et al. Criteria for assigning laboratory measurands to models for analytical performance specifications defined in the 1st EFLM Strategic Conference. Clin Chem Lab Med 2017;55:189–94.10.1515/cclm-2017-0772Search in Google Scholar


Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2019-0145).


Received: 2019-02-06
Accepted: 2019-05-14
Published Online: 2019-06-07
Published in Print: 2019-11-26

©2019 Walter de Gruyter GmbH, Berlin/Boston

Scroll Up Arrow