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Licensed Unlicensed Requires Authentication Published by De Gruyter December 5, 2019

Carryover issues with UF-5000 urine flow cytometry – how did we miss it?

Eline S. Andersen ORCID logo, Claus L. Brasen, Anne F. Christensen, Claus Østergaard and Ivan Brandslund

Corresponding author: Eline S. Andersen, MD, Department of Biochemistry and Immunology, Lillebaelt Hospital – University Hospital of Southern Denmark, Beriderbakken 4, 7100 Vejle, Denmark

  1. Author contributions: All authors revised the final manuscript. All authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The UF-5000 instrument and its reagents were supplied by Sysmex. All other expenses were held by the Department of Clinical Microbiology, the Emergency Department and the Department of Biochemistry and Immunology at Lillebaelt Hospital.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: Sysmex played no role in the study design, the collection, analysis or interpretation of data, in the writing of this report or in the decision to submit the report for publication.

References

1. Ren C, Wu J, Jin M, Wang X, Cao H. Rapidly discriminating culture-negative urine specimens from patients with suspected urinary tract infections by UF-5000. Bioanalysis 8 Oct 2018. DOI: 10.4155/bio-2018-0175. [Epub ahead of print].10.4155/bio-2018-0175Search in Google Scholar PubMed

2. De Rosa R, Grosso S, Lorenzi G, Bruschetta G, Camporese A. Evaluation of the new Sysmex UF-5000 fluorescence flow cytometry analyser for ruling out bacterial urinary tract infection and for prediction of Gram negative bacteria in urine cultures. Clin Chim Acta 2018;484:171–8.10.1016/j.cca.2018.05.047Search in Google Scholar PubMed

3. Kim SY, Park Y, Kim H, Kim J, Koo SH, Kwon GC. Rapid screening of urinary tract infection and discrimination of Gram-positive and Gram-negative bacteria by automated flow cytometric analysis using Sysmex UF-5000. J Clin Microbiol 2018;56:e02004–17.10.1128/JCM.02004-17Search in Google Scholar PubMed PubMed Central

4. Sysmex Corporation. Fully Automated Urine Particle Analyzer UF-5000 General Information. Kobe: Sysmex Corporation, 2016.Search in Google Scholar

5. Previtali G, Ravasio R, Seghezzi M, Buoro S, Alessio MG. Performance evaluation of the new fully automated urine particle analyser UF-5000 compared to the reference method of the Fuchs-Rosenthal chamber. Clin Chim Acta 2017;472:123–30.10.1016/j.cca.2017.07.028Search in Google Scholar PubMed

Received: 2019-09-23
Accepted: 2019-11-07
Published Online: 2019-12-05
Published in Print: 2020-03-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

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