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Influence of delayed separation of plasma from whole blood and centrifugation protocol on Zn plasma concentration

Mandy Leger , Quentin Despinasse , Patrice Faure , Josiane Arnaud , Corinne Ravelet and Benoit Chovelon EMAIL logo

Corresponding author: Benoit Chovelon, Institute of Biology and Pathology, University Hospital of Grenoble and Alps, CS 10217, Grenoble cedex 9, 38043, France, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: Research involving human subjects complied with all relevant national regulations, institutional policies and is in accordance with the tenets of the Helsinki Declaration (as revised in 2013), and has been approved by the authors’ Institutional Review Board or equivalent committee.


1. Killilea, DW, Rohner, F, Ghosh, S, Otoo, GE, Smith, L, Siekmann, JH, et al. Identification of a hemolysis threshold that increases plasma and serum zinc concentration. J Nutr 2017 Jun;147:1218–25. in Google Scholar

2. ab0a1/1464381209938/IZiNCGtechdocFNB2004.pdf published in International Zinc Nutrition Consultative Group. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 2004;25:S94–204.Search in Google Scholar

3. English, JL, Hambidge, KM. Plasma and serum zinc concentrations: effect of time between collection and separation. Clin Chim Acta 1988;175:211–5. in Google Scholar

4. World Health Organization. Use of anticoagulants in diagnostic laboratory investigations, WHO/DIL/LAB/99.1 rev.2. Geneva: WHO; 2002.Search in Google Scholar

5. Chovelon, B, Arnaud, J. Influence of delayed separation of plasma from whole blood on Cu, I, Mn, Se, and Zn plasma concentrations. Clin Chem Lab Med 2018;56:e69–71. in Google Scholar PubMed

6. Chovelon, B, Arnaud, J. Influence of temperature of transport of whole blood on plasma Cu, I, Mn, Se and Zn and Mg concentrations in erythrocytes. Clin Chem Lab Med 2019;57:e131–3. in Google Scholar

7. Accessed: 21 February 2020 Page 5 of 7. Clinical Chemistry and Laboratory Medicine.Search in Google Scholar

8. Oddoze, C, Lombard, E, Portugal, H. Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin Biochem 2012;45:464–9. in Google Scholar PubMed

9. Petersen, PH, Fraser, CG, Lund, F, Sölétormos, G. Confirmation of analytical performance characteristics required for the reference change value applied in patient monitoring. Scand J Clin Lab Invest 2015;75:628–30. in Google Scholar PubMed

10. Arnaud, J, Weber, JP, Weykamp, CW, Parsons, PJ, Angerer, J, Mairiaux, E, et al. Quality specifications for the determination of copper, zinc, and selenium in human serum or plasma: evaluation of an approach based on biological and analytical variation. Clin Chem 2008 Nov;54:1892–9. in Google Scholar PubMed

Received: 2020-03-25
Accepted: 2020-06-02
Published Online: 2020-07-01
Published in Print: 2020-10-25

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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