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Licensed Unlicensed Requires Authentication Published by De Gruyter November 19, 2020

Vortex mixing to alleviate pseudothrombocytopenia in a blood specimen with platelet satellitism and platelet clumps

  • Chaicharoen Tantanate EMAIL logo

Corresponding author: Chaicharoen Tantanate, Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand, Phone: (+66) 2-419-6587, Fax: (+66) 2-418-1367, E-mail:

Funding source: Chalermphrakiat Grant

Acknowledgments

The author gratefully acknowledges the patient profile in this report for granting permission that details relating to her case be published in this report.

  1. Research funding: The author was supported by a Chalermphrakiat Grant from the Faculty of Medicine Siriraj Hospital, Mahidol University.

  2. Author contributions: The author accepts full responsibility for all aspects of this report.

  3. Competing interests: The author states no conflict of interest.

  4. Ethical approval: The local Institutional Review Board deemed the study exempt from review.

References

1. Field, EJ, MacLeod, I. Platelet adherence to polymorphs. Br Med J 1963;2:388–9. https://doi.org/10.1136/bmj.2.5353.388-b.Search in Google Scholar

2. Zandecki, M, Genevieve, F, Gerard, J, Godon, A. Spurious counts and spurious results on haematology analysers: a review. Part I: platelets. Int J Lab Hematol 2007;29:4–20. https://doi.org/10.1111/j.1365-2257.2006.00870.x.Search in Google Scholar PubMed

3. Bobba, RV, Doll, DC. Platelet satellitism as a cause of spurious thrombocytopenia. Blood 2012;119:4100. https://doi.org/10.1182/blood-2011-08-369173.Search in Google Scholar PubMed

4. Bizzaro, N, Goldschmeding, R, von dem Borne, AE. Platelet satellitism is Fc gamma RIII (CD16) receptor-mediated. Am J Clin Pathol 1995;103:740–4. https://doi.org/10.1093/ajcp/103.6.740.Search in Google Scholar PubMed

5. Christopoulos, C, Mattock, C. Platelet satellitism and alpha granule proteins. J Clin Pathol 1991;44:788–9. https://doi.org/10.1136/jcp.44.9.788.Search in Google Scholar PubMed PubMed Central

6. Salama, A. Autoimmune thrombocytopenia complicated by EDTA- and/or citrate-dependent pseudothrombocytopenia. Transfus Med Hemotherapy 2015;42:345–8. https://doi.org/10.1159/000437220.Search in Google Scholar PubMed PubMed Central

7. Kovacs, F, Varga, M, Pataki, Z, Rigo, E. Pseudothrombocytopenia with multiple anticoagulant sample collection tubes. Interv Med Appl Sci 2016;8:181–3. https://doi.org/10.1556/1646.8.2016.4.4.Search in Google Scholar PubMed PubMed Central

8. Dima, F, Salvagno, G, Danese, E, Veneri, D, Lippi, G. An unusual case of sodium citrate-dependent artifactual platelet count [Internet]. Interv Med Appl Sci 2019. Available from: https://akjournals.com/view/journals/1646/aop/article-10.1556-1646.11.2019.24/article-10.1556-1646.11.2019.24.xml [Cited 22 Sep 2020].Search in Google Scholar

9. Sakurai, S, Tanigawa, T, Nakahara, K, Takenaka, K, Ohkubo, A. Effects of kanamycin on platelet satellitism and leukocyte adhesion phenomena. Rinsho Byori 1995;43:142–8.Search in Google Scholar

10. Gulati, GL, Asselta, A, Chen, C. Using a vortex to disaggregate platelet clumps. Lab Med 1997;28:665–7. https://doi.org/10.1093/labmed/28.10.665.Search in Google Scholar

Received: 2020-09-24
Accepted: 2020-11-05
Published Online: 2020-11-19
Published in Print: 2021-04-27

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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