Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter September 17, 2021

Effect of non-linearity on rheumatoid factor assay in Beckman system IMMAGE800

Huaizhou Wang, Yi Zhou, Liling Lin, Rongrong Zhu, ChengWen He and Yanghua Qin ORCID logo

Corresponding authors: Yanghua Qin, Department of Infectious Diseases, Changhai Hospital, SMMU, Room 404, No. 9 Building, 168 Changhai Road, 200433, Shanghai, P.R. China; and ChengWen He, Department of Laboratory Medicine, Changhai Hospital, SMMU, Room 602, No. 5 Building, 168 Changhai Road, 200433, Shanghai, P.R. China, Phone: 086 02131161904, E-mail: (Y. Qin), (C. He)

Huaizhou Wang and Yi Zhou contributed equally to this work.

Funding source: Shanghai Youth Clinical Technician (Clinical Laboratory) Program

Award Identifier / Grant number: N/A

  1. Research funding: This study was supported by Shanghai Youth Clinical Technician (Clinical Laboratory) Program (Y. Qin).

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: This study used the remaining samples from the clinical laboratory and exempt informed consent.

  5. Ethical approval: This study was approved by the Medical Ethics Committee of Changhai Hospital.


1. EP06-A | Evaluation of linearity of quantitative measurement procedures: a statistical approach [Online]. Available from: [Cited 23 Apr 2021].Search in Google Scholar

2. Anderson, SG, Bentzon, MW, Houba, V, Krag, P. International reference preparation of rheumatoid arthritis serum. Bull World Health Organ 1970;42:311–8.Search in Google Scholar

3. Miller, WG, Budd, J, Greenberg, N, Weykamp, C, Althaus, H, Schimmel, H, et al.. IFCC working group recommendations for correction of bias caused by noncommutability of a certified reference material used in the calibration hierarchy of an end-user measurement procedure. Clin Chem 2020;66:769–78. in Google Scholar PubMed PubMed Central

4. Hayashi, N, Kawano, S, Sugiyama, D, Nishimura, K, Nakazawa, T, Morinobu, A, et al.. Overestimation of serum levels of rheumatoid factor caused by the presence of an incorrect calibrator in the Dade Behring kit. Mod Rheumatol 2007;17:447–9. in Google Scholar

5. Painter, PC, Lyon, JM, Evans, JH, Powers, WW, Whittaker, RL, Decker, MJ. Performance of a new rate-nephelometric assay for rheumatoid factor, and its correlation with tube-titer results for human sera and synovial fluid. Clin Chem 1982;28:2214–8. in Google Scholar

6. Ameratunga, R, Musaad, S, Sugrue, C, Kyle, C. Rheumatoid factor measurement—continuing problems 70 years after discovery. Clin Rheumatol 2011;30:1215–20. in Google Scholar PubMed

7. Falkenburg, WJJ, von Richthofen, HJ, Koers, J, Weykamp, C, Schreurs, MWJ, Bakker-Jonges, LE, et al.. Clinically relevant discrepancies between different rheumatoid factor assays. Clin Chem Lab Med 2018;56:1749–58. in Google Scholar PubMed

8. Akbas, N, Budd, JR, Klee, GG. Multiple calibrator measurements improve accuracy and stability estimates of automated assays. Scand J Clin Lab Invest 2016;76:177–80. in Google Scholar PubMed

9. Vercammen, M, Meirlaen, P, Broodtaerts, L, Vande Broek, I, Bossuyt, X. Effect of sample dilution on serum free light chain concentration by immunonephelometric assay. Clin Chim Acta 2011;412:1798–804. in Google Scholar PubMed

10. Jonsson, PA. Sample dilution causes a positive bias on C-reactive protein using VITROS reagents, diluents and instruments—clinical implications? Clin Chem Lab Med 2008;46:1660–1. in Google Scholar PubMed

Received: 2021-04-23
Accepted: 2021-09-05
Published Online: 2021-09-17
Published in Print: 2022-01-26

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Scroll Up Arrow